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    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
    65.
    发明授权
    Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand 有权
    通过与核酸配体的竞争来测定非核酸分子与靶标的结合

    公开(公告)号：US06670132B2

    公开(公告)日：2003-12-30

    申请号：US10021330

    申请日：2001-12-10

    申请人： Nebojsa Janjic ,  Larry Gold

    发明人： Nebojsa Janjic ,  Larry Gold

    IPC分类号： C12Q168

    CPC分类号： G01N33/53 ,  C12Q1/6811 ,  Y10S530/812 ,  C12Q2545/107 ,  C12Q2565/518

    摘要： A competitive binding assay is used to determine whether a non-nucleic acid molecule from a library of non-nucleic acid molecules binds to a target. The non-nucleic acid molecule competes with a nucleic acid ligand for binding to the target which may be immobilized. Detecting displacement of nucleic acid ligand from a complex of the nucleic acid ligand and the target determines binding of the non-nucleic acid molecule to the target. The nucleic acid ligand may be immobilized and contacted with a target to form a complex. Adding a non-nucleic acid molecule to the complex displaces target from the complex, and detecting displacement of the target determines binding of the non-nucleic acid molecule to the target. Labeled nucleic acid ligand or labeled target displaced from or remaining in the complex can be detected for detecting displacement. Nucleic acid ligands that bind to the target are identified by the SELEX method. The assay provides a high throughput screening method for determining whether a non-nucleic acid molecule binds to a target.

    摘要翻译： 使用竞争性结合测定来确定非核酸分子的文库中的非核酸分子是否与靶标结合。 非核酸分子与核酸配体竞争结合靶，其可以被固定化。 检测核酸配体从核酸配体和靶的复合物的置换确定非核酸分子与靶标的结合。 核酸配体可以被固定并与靶接触以形成复合物。 向复合物中加入非核酸分子从复合物中取代靶，并且检测靶的位移确定非核酸分子与靶的结合。 可以检测标记的核酸配体或从复合物中残留的残留的标记的靶标，以检测位移。 通过SELEX法鉴定与靶标结合的核酸配体。 该测定提供了用于确定非核酸分子是否结合靶的高通量筛选方法。

    Nucleic acid ligand diagnostic biochip
    67.
    发明授权
    Nucleic acid ligand diagnostic biochip 有权
    核酸配体诊断生物芯片

    公开(公告)号：US06503715B1

    公开(公告)日：2003-01-07

    申请号：US09723394

    申请日：2000-11-28

    申请人： Larry Gold ,  Dan Drolet ,  Dom Zichi ,  Sumedha Jayasena ,  Steve Creighton ,  Stanley Gill

    发明人： Larry Gold ,  Dan Drolet ,  Dom Zichi ,  Sumedha Jayasena ,  Steve Creighton ,  Stanley Gill

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6839 ,  C07B2200/11 ,  C12Q1/6834 ,  C12Q1/6837 ,  C40B40/00 ,  Y10S977/924 ,  C12Q2565/514 ,  C12Q2565/101 ,  C12Q2541/101 ,  C12Q2525/205

    摘要： A Nucleic acid ligand “Biochip” is disclosed, consisting of a solid support to which one or more specific Nucleic acid ligands is attached in a spatially defined manner. Each Nucleic acid ligand binds specifically and avidly to a particular Target molecule contained within a Test mixture, such as a Bodily fluid. The Target molecules include, but are not limited to, proteins (cellular, viral, bacterial, etc.) hormones, sugars, metabolic byproducts, cofactor, and intermediates, drugs, and toxins. Contacting the Test mixture with the Biochip leads to the binding of a Target molecule to its cognate Nucleic acid ligand. Binding of Target to the Nucleic acid ligand results in a detectable change at each specific location on the Biochip. The detectable change can include, but is not limited to, a change in fluorescence, or a change in a physical parameter, such as electrical conductance or refractive index, at each location on the Biochip. The Biochip will then be read by a device, such as a fluorescence scanner or a surface plasmon resonance detector, that can measure the magnitude of the change at each location on the Biochip. The location of the change reveals what Target molecule has been detected, and the magnitude of the change indicates how much of it is present. The combination of these two pieces of information will yield diagnostic and prognostic medical information when signal patterns are compared with those obtained from Bodily fluids of individuals with diagnosed disorders (FIG. 1). In principle, the Biochip could be used to test any chemically complex mixture provided that Nucleic acid ligands to components suspected of being present in the mixture are attached to the Biochip. Thus, the Nucleic acid ligand Biochip will have a wider use in environmental testing, etc.

    摘要翻译： 披露了一种核酸配体“生物芯片”，由一种或多种特定的核酸配体以空间限定的方式连接的固体支持物组成。 每个核酸配体特异性地和特异性地结合到包含在测试混合物例如体液内的特定靶分子。 靶分子包括但不限于蛋白质（细胞，病毒，细菌等）激素，糖，代谢副产物，辅因子和中间体，药物和毒素。 将测试混合物与Biochip接触导致Target分子与其同源核酸配体的结合。 靶向核酸配体的结合在Biochip上的每个特定位置产生可检测的变化。 可检测的变化可以包括但不限于在生物芯片上的每个位置的荧光的变化或物理参数的变化，例如电导率或折射率。 然后，生物芯片将被诸如荧光扫描仪或表面等离子体共振检测器的装置读取，该检测器可以测量Biochip上每个位置的变化幅度。 变化的位置揭示了目标分子被检测到的，变化的大小表明它有多少存在。 当将信号模式与从具有诊断障碍的个体的体液获得的信号模式进行比较时，这两条信息的组合将产生诊断和预后的医学信息（图1）。 原则上，生物芯片可以用于测试任何化学复合物混合物，只要核酸配体与怀疑存在于混合物中的组分连接在Biochip上即可。 因此，核酸配体生物芯片将在环境测试等方面有更广泛的应用。