公开(公告)号：US08759033B2

公开(公告)日：2014-06-24

申请号：US13762984

申请日：2013-02-08

Applicant: Socpra Sciences et Genie S.E.C.

Inventor： Ryszard Brzezinski ,  Marie-Pierre Dubeau

IPC: C12N1/21 ,  C12N15/76

CPC classification number: C12N15/76 ,  C12N9/2434 ,  C12N9/2442 ,  C12Y302/01132

Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.

Abstract translation: 本发明涉及用于生产具有壳聚糖酶活性的酶的转基因细菌。 遗传修饰的放线菌具有减少（或消除）CsnR多肽的活性。 通过降低表达csnR基因，其相应转录物或表达显性负性CsnR多肽的能力可以获得这种降低的活性。 在用于生产具有壳聚糖酶活性的酶的培养基中壳聚糖的存在下，这种经遗传修饰的放线菌不太依赖（并且在一些实施方案中完全独立）。 此外，转基因细菌在培养基中产生的蛋白酶较少，最终提供了更高纯度的壳聚糖酶终产物。

公开(公告)号：US20130210077A1

公开(公告)日：2013-08-15

申请号：US13762984

申请日：2013-02-08

Applicant: SOCPRA SCIENCES ET GENIE S.E.C.

Inventor： Ryszard Brzezinski ,  Marie-Pierre Dubeau

IPC: C12N9/24

CPC classification number: C12N15/76 ,  C12N9/2434 ,  C12N9/2442 ,  C12Y302/01132

Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.

Abstract translation: 本发明涉及用于生产具有壳聚糖酶活性的酶的转基因细菌。 遗传修饰的放线菌具有减少（或消除）CsnR多肽的活性。 通过降低表达csnR基因，其相应转录物或表达显性负性CsnR多肽的能力可以获得这种降低的活性。 在用于生产具有壳聚糖酶活性的酶的培养基中壳聚糖的存在下，这种经遗传修饰的放线菌不太依赖（并且在一些实施方案中完全独立）。 此外，转基因细菌在培养基中产生的蛋白酶较少，最终提供了更高纯度的壳聚糖酶终产物。