Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data

    公开(公告)号:US20060047443A1

    公开(公告)日:2006-03-02

    申请号:US11217694

    申请日:2005-09-01

    IPC分类号: G06F19/00 C12Q1/68

    CPC分类号: C12Q1/6851

    摘要: Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

    Apparatus for estimating a half-pel motion in a video compression method
    62.
    发明授权
    Apparatus for estimating a half-pel motion in a video compression method 失效
    用于在视频压缩方法中估计半像素运动的装置

    公开(公告)号:US5694179A

    公开(公告)日:1997-12-02

    申请号:US717733

    申请日:1996-09-19

    IPC分类号: H04N7/36 H04N7/32

    CPC分类号: H04N19/523

    摘要: An apparatus for estimating a half-pel motion comprising: a memory for storing motion-compensation errors in integer-pel positions; an address generator for generating an address of the integer-pel positions to the memory; an operator for receiving the motion-compensation errors from the memory, and calculating motion-compensation errors in the eight half-pel positions which are on the outskirts of the integer-pel position having a minimum motion-compensation error; and a comparator and half-pel motion vector selector for comparing the motion-compensation errors in the eight half-pel positions with the minimum motion-compensation error in the integer-pel position and selecting a position having a minimum motion-compensation error as a final half-pel motion vector.

    摘要翻译: 一种用于估计半像素运动的装置,包括:用于存储整数像素位置的运动补偿误差的存储器; 地址发生器,用于向存储器生成整数像素位置的地址; 用于从存储器接收运动补偿误差的运算器,以及计算在具有最小运动补偿误差的整数像素位置的郊外的八个半像素位置中的运动补偿误差; 以及用于将八个半像素位置中的运动补偿误差与整数像素位置中的最小运动补偿误差进行比较的比较器和半像素运动矢量选择器,并且将具有最小运动补偿误差的位置选择为 最终半像素运动矢量。