摘要:
Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.
摘要:
An apparatus for estimating a half-pel motion comprising: a memory for storing motion-compensation errors in integer-pel positions; an address generator for generating an address of the integer-pel positions to the memory; an operator for receiving the motion-compensation errors from the memory, and calculating motion-compensation errors in the eight half-pel positions which are on the outskirts of the integer-pel position having a minimum motion-compensation error; and a comparator and half-pel motion vector selector for comparing the motion-compensation errors in the eight half-pel positions with the minimum motion-compensation error in the integer-pel position and selecting a position having a minimum motion-compensation error as a final half-pel motion vector.