公开(公告)号：US5348636A

公开(公告)日：1994-09-20

申请号：US148239

申请日：1993-11-08

申请人： Gary Slater ,  Jaan Noolandi ,  Chantal Turmel

发明人： Gary Slater ,  Jaan Noolandi ,  Chantal Turmel

IPC分类号： B01D57/02 ,  C07H1/08 ,  G01N27/447 ,  G01N27/26

CPC分类号： B01D57/02 ,  G01N27/44773

摘要： A process for the electrophoretic separation of charged macromolecules includes applying to the macromolecules a periodic sequence of pulses. Each period comprises a multiplicity of electric field pulses of negative and positive polarities. The negative polarity pulses are applied for a longer total time duration than the positive polarity pulses within each period. The average intensity of the negative polarity pulses is less than the average intensity of the positive polarity pulses.

摘要翻译： 电荷分离电荷大分子的方法包括向大分子施加周期性的脉冲序列。 每个周期包括负极性和正极性的多个电场脉冲。 与每个周期内的正极性脉冲相比，施加负极性脉冲的总持续时间更长。 负极性脉冲的平均强度小于正极性脉冲的平均强度。

公开(公告)号：US5286434A

公开(公告)日：1994-02-15

申请号：US783967

申请日：1991-10-29

申请人： Gary Slater ,  Jaan Noolandi ,  Chantal Turmel

发明人： Gary Slater ,  Jaan Noolandi ,  Chantal Turmel

IPC分类号： B01D57/02 ,  C07H1/08 ,  G01N27/447 ,  G01N27/26

CPC分类号： B01D57/02 ,  G01N27/44773

摘要： A process for the electrophoretic separation of charged macromolecules includes applying to the macromolecules a periodic sequence of pulses. Each period comprises a multiplicity of electric field pulses of negative and positive polarities. The negative polarity pulses are applied for a longer total time duration than the positive polarity pulses within each period. The average intensity of the negative polarity pulses is less than the average intensity of the positive polarity pulses.

公开(公告)号：US5167784A

公开(公告)日：1992-12-01

申请号：US754854

申请日：1991-09-04

申请人： Jaan Noolandi

发明人： Jaan Noolandi

IPC分类号： C12Q1/68 ,  G01N27/447

CPC分类号： G01N27/44773

摘要： The base sequence of large single stranded nucleic acids is determined by retarding the migration rate of a nucleic acid fragment of n bases below a migration rate which would otherwise be the same as the migration rate of a nucleic acid fragment of n bases in a polyacrylamide gel under continuous field gel electrophoresis. A plurality of sequences of electric field pulses is applied to the gel in one dimension.