公开(公告)号：US06280992B1

公开(公告)日：2001-08-28

申请号：US09404056

申请日：1999-09-23

申请人： Richard D. Morgan ,  Qing Xu

发明人： Richard D. Morgan ,  Qing Xu

IPC分类号： C12N922

CPC分类号： C12N9/22

摘要： In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J99 (NEB#1237), hereinafter referred to as “Hpy99I”, which endonuclease: (1) recognizes the nucleotide sequence 5′-CGWCG-3′ in a double-stranded DNA (wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and W represents either A, or T); (2) cleaves double-stranded PhiX174 DNA to produce 8 fragments, including fragments of 3063, 629, 602, 447, 389, and 176 base pairs, and 2 fragments smaller than 100 base pairs.

摘要翻译： 本发明提供了可从幽门螺杆菌J99（NEB＃1237）获得的新型限制性内切核酸酶及其DNA，以下称为“Hpy99I”，该内切核酸酶：（1）识别核苷酸序列5'-CGWCG -3'（其中G表示鸟嘌呤，C表示胞嘧啶，A表示腺嘌呤，T表示胸腺嘧啶，W表示A或T）;（2）切割双链PhiX174 DNA以产生8个片段， 包括3063,629,602,447,389和176个碱基对的片段，以及小于100个碱基对的2个片段。

公开(公告)号：US06258583B1

公开(公告)日：2001-07-10

申请号：US09393245

申请日：1999-09-10

申请人： Richard D. Morgan ,  Qing Xu

发明人： Richard D. Morgan ,  Qing Xu

IPC分类号： C12N922

CPC分类号： C12N9/22

摘要： In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J188 (NEB#1174), hereinafter referred to as “Hpy188I”, which endonuclease: (1) recognizes the nucleotide sequence 5′-TCNGA-3′ in a double-stranded DNA molecule as shown below, 5′-TCN↓GA-3′ 3′-AG↑NCT-5′ (wherein G represents guanine, C represents cytosine, A represents adenine, T represents thymine and N represents either G, C, A, or T); (2) cleaves said sequence in the phosphodiester bonds between the N and G as indicated with the arrows; and (3) cleaves double-stranded PhiX174 DNA to produce 18 fragments, including fragments of 1331, 813, 572, 525, 396, 302, 293, 219 base pairs, and 10 fragments smaller than 200 base pairs.

摘要翻译： 根据本发明，提供了可从幽门螺杆菌J188（NEB＃1174）获得的新型限制性内切核酸酶及其DNA，以下称为“Hpy188I”，其内切核酸酶：（1）识别核苷酸序列5'-TCNGA （其中G表示鸟嘌呤，C表示胞嘧啶，A表示腺嘌呤，T表示胸腺嘧啶，N表示G，C，A或T）;（2）将所述 N和G之间的磷酸二酯键合序列如箭头所示; 并且（3）切割双链PhiX174DNA以产生18个片段，包括1331,813,572,525,396,302,293,219碱基对的片段和小于200个碱基对的10个片段。