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81.发明申请Variants And Compositions Comprising Variants With High Stability In Presence Of A Chelating Agent 有权含有高度稳定性的变体和组合物在螯合剂存在下公开(公告)号：US20110195481A1
公开(公告)日：2011-08-11
申请号：US13024770
申请日：2011-02-10
申请人： Allan Svendsen , Annette Helle Johansen , Mads Bjoernvad , Frank W. Rasmussen , Michael Skjoet , Signe Eskildsen Larsen , Jens Oebro , Svend Kaasgaard , Lars Beier
发明人： Allan Svendsen , Annette Helle Johansen , Mads Bjoernvad , Frank W. Rasmussen , Michael Skjoet , Signe Eskildsen Larsen , Jens Oebro , Svend Kaasgaard , Lars Beier
CPC分类号： C12N9/2417
摘要： The present invention relates to variants of an alpha-amylase having improved stability to chelating agents relative to its parent enzyme, compositions comprising the variants, nucleic acids encoding the variants, methods of producing the variants, and methods for using the variants.
摘要翻译： 本发明涉及具有相对于其母体酶对螯合剂具有改善的稳定性的α-淀粉酶的变体，包含变体的组合物，编码变体的核酸，产生变体的方法以及使用该变体的方法。
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82.发明授权Pullulanase variants and methods for preparing such variants with predetermined properties 失效支链淀粉酶变体和用于制备具有预定性质的这种变体的方法公开(公告)号：US07906306B2
公开(公告)日：2011-03-15
申请号：US12202808
申请日：2008-09-02
申请人： Allan Svendsen
发明人： Allan Svendsen
CPC分类号： C12N9/2457 , C12N9/246 , C12Y302/01041 , C12Y302/01068
摘要： The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or altered cleavage pattern.
摘要翻译： 本发明涉及支链淀粉酶变体，其中变体具有改善的性质，例如改变的pH最佳，改善的热稳定性，改变的底物特异性，增加的比活性或改变的切割模式。
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公开(公告)号：US20100135978A1
公开(公告)日：2010-06-03
申请号：US12693947
申请日：2010-01-26
CPC分类号： C12N9/54
摘要： The pharmaceutical use of proteases related to amino acids 1-274 of SEQ ID NO: 2, the serine protease derived from Bacillus licheniformis, which is also designated subtilisin Carlsberg, optionally in combination with a lipase and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II.
摘要翻译： 与SEQ ID NO：2的氨基酸1-274相关的蛋白酶的药物用途，来自地衣芽孢杆菌的丝氨酸蛋白酶，其也被指定为枯草杆菌蛋白酶卡尔斯伯格，任选与脂肪酶和/或淀粉酶组合。 医学适应症的例子有：消化系统疾病，胰腺外分泌功能不全（PEI），胰腺炎，囊性纤维化，I型糖尿病和/或II型糖尿病的治疗。
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公开(公告)号：US20090203108A1
公开(公告)日：2009-08-13
申请号：US12413758
申请日：2009-03-30
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质。 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US07566561B2
公开(公告)日：2009-07-28
申请号：US10327837
申请日：2002-12-23
申请人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
发明人： Allan Svendsen , Torben Vedel Borchert , Henrik Bisgard-Frantzen , Helle Outtrup , Bjarne Ronfeldt Nielsen , Vibeke Skovgaard Nielsen , Lisbeth Hedegaard
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38609 , C11D3/38618 , C12Y302/01001
摘要： The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).
摘要翻译： 本发明涉及一种新的类戊酰样α-淀粉酶和包含2,3,4,5或6个或6个位置突变的类戊酰样α-淀粉酶。 这些变体在酸性pH和/或低Ca 2+浓度（相对于亲本）下具有增加的热稳定性。 本发明还涉及包含编码本发明的α-淀粉酶变体的DNA序列的DNA构建体，携带本发明的DNA构建体的重组表达载体，用本发明的DNA构建体转化的细胞，使用 本发明的α-淀粉酶变体用于洗涤和/或餐具洗涤，纺织品退浆，淀粉液化，包含本发明的α-淀粉酶变体的洗涤剂添加剂，手动或自动洗碗洗涤剂组合物，其包含α-淀粉酶变体 本发明提供一种产生母体类戊酰样α-淀粉酶的变体的方法，该变体在酸性pH和/或低Ca 2+浓度（相对于亲本）中表现出增加的热稳定性。
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公开(公告)号：US20090148893A1
公开(公告)日：2009-06-11
申请号：US11928576
申请日：2007-10-30
申请人： Allan Svendsen , Henriette Draborg
发明人： Allan Svendsen , Henriette Draborg
CPC分类号： C12N9/54 , C11D3/386 , C12Y304/21062
摘要： The present invention relates to methods for producing variants of a parent TY145 subtilase and of a parent BPN′ subtilase and to TY145 and BPN′ variants having altered properties as compared to the parent TY145/BPN′ subtilase.
摘要翻译： 本发明涉及用于产生亲本TY145脱落酶和亲本BPN'脱羧酶的变体的方法，并且与亲本TY145 / BPN'脱羧酶相比具有改变的性质的TY145和BPN'变体的方法。
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公开(公告)号：US07537921B2
公开(公告)日：2009-05-26
申请号：US10537746
申请日：2003-12-11
申请人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
发明人： Leonardo De Maria , Allan Svendsen , Torben Vedel Borchert , Lars Lehmann Hylling Christensen , Sine Larsen , Carsten Ryttersgaard
CPC分类号： C12N9/2468 , C12Y302/01089
摘要： Variants of Glycoside Hydrolase family 53 galactanases, e.g. variants of the galactanases from strains of Yersinia, Aspergillus, Humicola, Meripilus, Myceliophthora, Thermomyces, Bacillus, Bifidobacterium, Cellvibrio, Clostridium, Pseudomonas, Thermotoga, or Xanthomonas.
摘要翻译： 糖苷类水解酶家族53半乳聚糖酶的变体，例如 来自耶尔森氏菌属，曲霉属，腐质霉属，Meripilus，Myceliophthora，Thermomyces，Bacillus，Bifidobacterium，Cellvibrio，Clostridium，Pseudomonas，Thermotoga或Xanthomonas的菌株的半乳聚糖酶的变体。
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公开(公告)号：US07326554B2
公开(公告)日：2008-02-05
申请号：US10734510
申请日：2003-12-12
申请人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehman , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
发明人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehman , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
IPC分类号： C12N9/16
CPC分类号： C12N15/8257 , A23K10/14 , A23K20/189 , C12N9/16 , C12N15/8242 , C12N15/8243 , Y10S435/911 , Y10S435/916
摘要： The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.
摘要翻译： 本发明涉及植酸酶变体，其制备和用途，当植酸酶变体在根据图1对齐时。 与在多个位置中的至少一个中的模型植酸酶相比，修改了1。 优选的植酸酶类型是担子菌和子囊菌植酸酶，如Peniophora植酸酶和曲霉属植酸酶。 优选的植酸酶变体表现出修正的活性特征，例如提高的比活性和/或改善的热稳定性。
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公开(公告)号：US20070128313A1
公开(公告)日：2007-06-07
申请号：US11633385
申请日：2006-12-04
IPC分类号： A21D8/02 , C12C11/00 , C12P21/06 , C12P19/02 , C12P7/06 , C12N9/32 , C07H21/04 , C12N15/74 , C12N1/16
CPC分类号： C12Y302/01001 , A21D8/042 , C12C5/004 , C12C11/00 , C12N9/2417 , C12N9/242 , C12P7/06 , C12P19/02 , C12P19/14 , C13K1/06 , C13K7/00 , Y02E50/17
摘要： The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.
摘要翻译： 本发明涉及亲本的Fungamyl样真菌α-淀粉酶的变体，其在适于例如淀粉方法的酸性pH下表现出改进的热稳定性。
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公开(公告)号：US20050181446A1
公开(公告)日：2005-08-18
申请号：US09957806
申请日：2001-09-21
申请人： Erwin Roggen , Steffen Ernst , Allan Svendsen , Esben Friis , Claus Osten
发明人： Erwin Roggen , Steffen Ernst , Allan Svendsen , Esben Friis , Claus Osten
IPC分类号： A21D2/26 , A21D8/04 , C07K1/04 , C07K5/103 , C07K5/11 , C07K5/113 , C07K14/00 , C07K16/40 , C07K16/42 , C11D3/386 , C12N9/02 , C12N9/20 , C12N15/53 , C12N15/55 , C12Q1/70 , C12N5/06 , C12P21/02 , G01N33/48 , G01N33/50 , G01N33/53 , G06F19/00
CPC分类号： C12N9/54 , A21D2/267 , A21D8/042 , A61K39/00 , C07K1/047 , C07K5/1008 , C07K5/101 , C07K5/1013 , C07K5/1019 , C07K5/1021 , C07K14/001 , C07K16/40 , C07K16/4283 , C11D3/38627 , C11D3/38645 , C12N9/0061 , C12N9/20 , C12Y110/03002 , C12Y304/21062 , Y10S435/911
摘要： The present invention relates to a method of selecting a protein variant having modified immunogenicity as compared to the parent protein comprising the steps obtaining antibody binding peptide sequences, using the sequences to localise epitope sequences on the 3-dimensional structure of parent protein, defining an epitope area including amino acids situated within 5 Å from the epitope amino acids constituting the epitope sequence, changing one or more of the amino acids defining the epitope area of the parent protein by genetical engineering mutations of a DNA sequence encoding the parent protein, introducing the mutated DNA sequence into a suitable host, culturing said host and expressing the protein variant, and evaluating the immunogenicity of the protein variant using the parent protein as reference. The invention further relates to the protein variant and use thereof, as well as to a method for producing said protein variant.
摘要翻译： 本发明涉及选择与亲本蛋白相比具有改良的免疫原性的蛋白质变体的方法，其包括获得抗体结合肽序列的步骤，使用该序列来定位母体蛋白的3维结构上的表位序列，限定表位 包括位于构成表位序列的表位氨基酸5埃范围内的氨基酸，通过编码母体蛋白质的DNA序列的遗传工程突变改变限定母体蛋白表位区域的一个或多个氨基酸，引入突变的 DNA序列转化为合适的宿主，培养所述宿主并表达蛋白质变体，并使用亲本蛋白作为参考来评估蛋白质变体的免疫原性。 本发明还涉及蛋白质变体及其用途，以及生产所述蛋白质变体的方法。
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