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公开(公告)号：US3986930A
公开(公告)日：1976-10-19
申请号：US579910
申请日：1975-05-22
CPC分类号： C12Q1/44 , C07C327/00
摘要： A reagent for determining lipase activity, which comprises as the active ingredient one of the S-acyl compounds of the formula: ##EQU1## WHEREIN N IS AN INTEGER OF 2 TO 4, INCLUSIVE; N' IS AN INTEGER OF 2 TO 4, INCLUSIVE OR OF 9 TO 13, INCLUSIVE; M IS AN INTEGER OF 3, 7 OR 11; M' IS AN INTEGER OF 4 OR 12; Y IS AN INTEGER OF 1 OR 2; X is sulfur atom or oxygen atom; Y is sulfur atom, oxygen atom or ethylene group; provided that when y is 1, m' is 4 and when y is 2, m' is 12, and a method for determining lipase activity by reacting the S-acyl compound with lipase in a buffer solution containing a chromogenic sulfhydryl reagent and then subjecting the resultant to a colorimetry.
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公开(公告)号：US3983005A
公开(公告)日：1976-09-28
申请号：US565897
申请日：1975-04-07
CPC分类号： C12Q1/60 , Y10S435/805 , Y10S435/839 , Y10S435/897 , Y10S435/918
摘要： An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, a support. Cholesterol oxidase and a composition for the hydrolysis of cholesterol esters comprising lipase having cholesterol esterase activity and protease are included in the element such that cholesterol esters contained in a sample applied to the spreading layer are saponified to free cholesterol and free cholesterol is decomposed in the presence of cholesterol oxidase to produce a detectable change related to the total cholesterol content of the sample.
摘要翻译： 公开了用于分析其胆固醇含量的液体的整体分析元件。 该元件是包括至少两个叠加层的类型，包括扩散层和流体接触的试剂层，以及任选的支撑体。 胆固醇氧化酶和用于水解包含具有胆固醇酯酶活性的脂肪酶的胆固醇酯和蛋白酶的组合物包括在元件中，使得包含在施加到铺展层上的样品中的胆固醇酯被皂化以游离胆固醇并且游离胆固醇在存在下分解 的胆固醇氧化酶以产生与样品的总胆固醇含量相关的可检测的变化。
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公开(公告)号：US3891573A
公开(公告)日：1975-06-24
申请号：US46564074
申请日：1974-04-30
发明人： STARY EMIL , ROESCHLAU PETER , BERNT ERICH
CPC分类号： G01N33/96 , C12Q1/60 , G01N33/92 , Y10T436/104165 , Y10T436/108331
摘要： Highly stable cholesterol standard solution comprising a known amount of cholesterol together with 1 to 20 volume percent of a primary or secondary aliphatic alcohol containing up to 4 carbon atoms; 5 to 20 volume percent hydroxy-polyethoxydodecane or 40 to 60 volume percent diethyleneglycol monobutyl ether; and 0.5 to 5 weight percent sodium chloride and/or 0.01 to 0.5 weight percent alkyl dimethyl benzyl ammonium chloride with 8 to 18 carbon atoms in the alkyl radicals.
摘要翻译： 包含已知量的胆固醇和1至20体积％含有至多4个碳原子的伯或仲脂族醇的高度稳定的胆固醇标准溶液; 5至20体积％的羟基 - 聚乙氧基十二烷或40至60体积％的二甘醇单丁醚; 和0.5至5重量％的氯化钠和/或0.01至0.5重量％的在烷基中具有8至18个碳原子的烷基二甲基苄基氯化铵。
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公开(公告)号：US11486875B2
公开(公告)日：2022-11-01
申请号：US17023946
申请日：2020-09-17
申请人： SYSMEX CORPORATION
发明人： Keiko Yoshikawa , Amane Harada , Katsuhiro Murakami , Maria Kiriyama , Keiko Miwa , Takuya Kubo
摘要： The present invention relates to a method for measuring the cholesterol uptake capacity of lipoproteins. The present invention also relates to a reagent kit for measuring the cholesterol uptake capacity of lipoproteins. The present invention further relates to a tagged cholesterol which can be used in the method and the reagent kit.
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公开(公告)号：US11268130B2
公开(公告)日：2022-03-08
申请号：US16530322
申请日：2019-08-02
发明人： Douglas Matje , Ian Gibbons , Paul Patel , Elizabeth A. Holmes
摘要： Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.
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86.发明申请公开(公告)号：US20190284527A1
公开(公告)日：2019-09-19
申请号：US16301494
申请日：2017-05-30
摘要： The present invention provides a co-culture system and method for assessing cellular cholesterol (Choi) efflux and uptake in vitro. The co-culture system mimics in vivo Choi efflux and uptake in the context of mammalian physiology. The methods and systems provided can be used in some embodiments to evaluate the effect of a pharmacological agent on cellular Choi efflux and uptake or for diagnostic purposes.
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公开(公告)号：US10392650B2
公开(公告)日：2019-08-27
申请号：US15364762
申请日：2016-11-30
发明人： Douglas Matje , Ian Gibbons , Paul Patel , Elizabeth A. Holmes
摘要： Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures.
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公开(公告)号：US20190128902A1
公开(公告)日：2019-05-02
申请号：US16222845
申请日：2018-12-17
发明人： Ian GIBBONS , Shaunak ROY , Edmond KU
摘要： This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.
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89.发明授权公开(公告)号：US09982289B2
公开(公告)日：2018-05-29
申请号：US13105375
申请日：2011-05-11
申请人： Karen L. Marfurt
发明人： Karen L. Marfurt
CPC分类号： C12Q1/54 , C12Q1/60 , C12Q1/61 , C12Q1/62 , G01N15/00 , G01N21/8483 , G01N33/50 , G01N33/523
摘要： A test strip or electrochemical sensor for measuring the amount of an analyte in a biological fluid, e.g. the glucose content of whole blood, includes a size self-limiting reagent formulation employing an enzyme system for reaction with the analyte, the reactive system mixed into a water-soluble swellable polymer matrix containing small water-insoluble particles having a nominal size of about 0.05 to 20 μm, preferably about 1 to 10 μm. The weight ratio of the water-insoluble particles to the water-soluble swellable polymer matrix is about 1/2 to 2/1. The reagent formulation is deposited onto a non-porous substrate to form a thin layer about 6-16 μm thick, providing a rapid and stable response to application of a sample, while being insensitive to the amount of the sample.
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公开(公告)号：US09695464B2
公开(公告)日：2017-07-04
申请号：US14357561
申请日：2012-11-09
申请人： AXIS-SHIELD AS
摘要： The invention provides an enzymatic method for measuring the concentration of one or more analytes in the plasma portion of a blood derived sample, containing a first and a second component, where said second component interferes with the measurement of said first component. The method includes: i) diluting the sample with a reagent mixture; ii) substantially removing blood cells; iii) using a reagent which serves to temporarily prevent reaction of the second component, to generate a blocked second component; iv) causing the selective reaction of a constituent of each analyte to directly or indirectly generate detectable reaction products, where one of the analytes is the first component; v) monitoring the detectable reaction product or products; vi) relating an amount of the detectable product or products and/or a rate of formation of the detectable product or products to the concentration of each analyte, where the concentration of at least the first component is related to a corresponding detectable reaction product by means of estimating an un-measurable (fictive) endpoint. Step iii) may be carried out at any stage up to and including step iv) but before steps v) or vi). The reagent of step iii) may be applied to the sample separately or may be included in a reagent mixture during steps i) or iv). A corresponding kit is also provided.
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