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598 条记录 (耗时 0.037 秒)

    Differential dissociation and melting curve peak detection
    1.
    发明授权
    Differential dissociation and melting curve peak detection 有权

    公开(公告)号：US11257570B2

    公开(公告)日：2022-02-22

    申请号：US15965380

    申请日：2018-04-27

    申请人： Applied Biosystems, LLC

    发明人： Francis T. Cheng

    IPC分类号： G16B40/10 ,  G16B45/00 ,  G16B99/00 ,  C12Q1/6844

    摘要： Systems and methods are provided for processing a melting or dissociation curve of a DNA or other sample, for example, during PCR processing. In some embodiments, detection of the melting point and melting curve behavior can be enhanced by taking a derivative of the curve, and detecting peaks in the differential dissociation curve. In some embodiments, the derivative operation can comprise the use of edge-processing, or other detection algorithms. In some embodiments, the dissociation analysis can comprise removing low-frequency (or pedestal) components of the differential dissociation curve. In some embodiments, the differential dissociation curve can exhibit a smoothed or more regular appearance than the raw detected data.

    Compositions, methods, and kits for amplifying nucleic acids
    2.
    发明授权
    Compositions, methods, and kits for amplifying nucleic acids 有权

    公开(公告)号：US11225686B2

    公开(公告)日：2022-01-18

    申请号：US16746216

    申请日：2020-01-17

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Shoulian Dong ,  Junko F. Stevens ,  Danny H. Lee

    IPC分类号： C12P19/34 ,  C12N15/115 ,  C12Q1/6844 ,  C12Q1/6848 ,  G01N21/64

    摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

    Systems and methods for electronic detection with nanoFETs
    3.
    发明授权
    Systems and methods for electronic detection with nanoFETs 有权

    公开(公告)号：US11175258B2

    公开(公告)日：2021-11-16

    申请号：US16247069

    申请日：2019-01-14

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Mark F. Oldham ,  Eric S. Nordman

    IPC分类号： G01N27/414 ,  B82Y15/00 ,  C12Q1/6825 ,  G01N33/543

    摘要： There is disclosed a system for electrical charge detection comprising a nanoFET device. Also disclosed is a method of electrical charge detection for single molecule sequencing. The method includes attaching a macromolecule or assemblies thereof to a gate of a nanoFET device and flowing in a solution of charge tags, where a charge tag includes a nucleotide attached to a charge complex. The method also includes incorporating one charge tag into the macromolecule or assemblies thereof and cleaving the charge tags from the macromolecule or assemblies thereof. The method further includes detecting at least one of current and voltage from the nanoFET device.

    Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
    6.
    发明授权
    Methods, compositions, and kits comprising linker probes for quantifying polynucleotides 有权

    公开(公告)号：US10781486B2

    公开(公告)日：2020-09-22

    申请号：US15490323

    申请日：2017-04-18

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Caifu Chen ,  Dana Ridzon ,  Zhaohui Zhou ,  Kai Qin Lao ,  Neil A. Straus

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/6876 ,  C12N9/00 ,  C12Q1/686

    摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

    Sample block apparatus and method for maintaining a microcard on a sample block
    8.
    发明授权
    Sample block apparatus and method for maintaining a microcard on a sample block 有权

    公开(公告)号：US10253361B2

    公开(公告)日：2019-04-09

    申请号：US13972735

    申请日：2013-08-21

    申请人： APPLIED BIOSYSTEMS, LLC

    发明人： Adrian Fawcett

    IPC分类号： C12M1/34 ,  C12Q1/686 ,  B01L7/00 ,  B01L9/00

    摘要： A thermal cycling device for thermally cycling samples of biological material contained in a microcard having a top and bottom surface. The thermal cycling device can include a sample block having an upper surface configured for engaging the bottom surface of a microcard, a vacuum device, and a temperature control system operatively connected with the sample block. The upper surface of the sample block may include a plurality of channels, the channels defining spaces between the sample block and the bottom surface of a microcard that may be positioned thereon. The vacuum device may be in fluid communication with the sample block for drawing gas out of the spaces defined by the channels in the sample block. The vacuum device may be configured for substantially maintaining a vacuum between the sample block and microcard so that a retention force is imparted on the microcard to urge the microcard toward the sample block. Methods of maintaining a microcard on a sample block of a thermal cycling device are also provided.

    High Speed, High Resolution Compositions, Methods and Kits for Capillary Electrophoresis
    9.
    发明申请
    High Speed, High Resolution Compositions, Methods and Kits for Capillary Electrophoresis 审中-公开

    公开(公告)号：US20190064108A1

    公开(公告)日：2019-02-28

    申请号：US15960838

    申请日：2018-04-24

    申请人： Applied Biosystems, LLC

    发明人： Karl O. Voss ,  Aldrich N.K. Lau

    IPC分类号： G01N27/447 ,  B01D53/02

    摘要： The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.