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公开(公告)号：US11320350B2
公开(公告)日：2022-05-03
申请号：US16339655
申请日：2017-10-04
发明人： Shino Muramatsu , Daisuke Kato , Tomohiro Hattori
摘要： Methods of agglutinating and separating erythrocytes, by which erythrocytes can be instantaneously agglutinated into a sufficient size in a blood sample and completely separated from the blood sample; and a hemagglutination reagent are provided. The method of agglutinating erythrocytes according to the present invention includes adding a solution containing a cholic acid-based surfactant and an acid to a blood sample. The method of separating erythrocytes according to the present invention includes separating the erythrocytes agglutinated by the above-described method of the present invention. The hemagglutination reagent according to the present invention contains a cholic acid-based surfactant and an acid.
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公开(公告)号：US10436783B2
公开(公告)日：2019-10-08
申请号：US15569143
申请日：2016-04-28
发明人： Yuji Saito , Daisuke Kato
IPC分类号： G01N33/53 , G01N33/569 , C12N1/00 , C12Q1/04 , C12N1/06 , G01N33/543 , C07K1/36 , C07K14/195
摘要： This invention provides a method for easily collecting antigens possessed by microorganisms without the use of special equipment. The method for collecting microbial antigens comprises: allowing a specimen containing microorganisms to pass through a filter membrane with a pore diameter that does not allow microorganisms to pass therethrough; capturing the microorganisms in the specimen on the filter membrane; applying a microbial destruction reagent capable of microbial membrane destruction to the filter membrane comprising the microorganisms captured thereon to destruct the captured microorganisms on the filter membrane; and collecting antigens in the filtrate.
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3.发明申请公开(公告)号：US20190277838A1
公开(公告)日：2019-09-12
申请号：US16420594
申请日：2019-05-23
发明人： Risa Kohiyama , Osamu ISHIKAWA , Takashi MIYAZAWA
IPC分类号： G01N33/543 , G01N21/64 , G01N33/558 , G01N33/545 , B01D15/38
摘要： The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other. The immunochromatographic test piece is an immunochromatographic test piece comprising a membrane on which a capture substance being a ligand that bonds to a substance to be detected is immobilized, wherein insoluble carrier particles to which a ligand that bonds to the substance to be detected is bound are used and accumulated by being captured with the capture substance immobilized on the membrane, the membrane is irradiated with light to detect light emitted from a portion where the insoluble carrier particles are accumulated or light emitted from a portion surrounding and other than the portion where the insoluble carrier particles are accumulated, thereby measuring the substance to be detected, and a light-reflecting material is provided on a side of the membrane opposite to a side irradiated with light.
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4.发明申请公开(公告)号：US20190101532A1
公开(公告)日：2019-04-04
申请号：US16087485
申请日：2016-03-23
发明人： Yuki SHINOHARA , Miwa KUWAHARA , Hiroshi AKITA , Takashi MIYAZAWA
IPC分类号： G01N33/543 , G01N33/487 , G01N33/52 , G01N33/53 , G01N33/531
摘要： Disclosed are an immunochromatographic test device which allows a target substance to be detected or quantified more rapidly and sensitively than conventional methods, and a method of forming a sample addition part of the test device. The method of forming a sample addition part of the immunochromatographic test device includes applying a surfactant that is in white powder form under normal conditions, such as sodium deoxycholate, to the sample addition part and drying it. The immunochromatographic test device includes the sample addition part formed by the method.
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公开(公告)号：US10077286B2
公开(公告)日：2018-09-18
申请号：US14395449
申请日：2013-04-19
发明人： Motoko Ohta
CPC分类号： C07K1/14 , C12Q1/26 , C12Q1/44 , C12Q1/485 , C12Q1/61 , G01N33/6893 , G01N33/92 , G01N2800/32 , G01N2800/7085
摘要： Disclosed is a method for selectively eliminating triglycerides in lipoproteins other than low density lipoprotein, which method allows one to provide a method for directly and differentially quantifying LDL-TG in a sample with excellent simplicity, specificity and accuracy using an automated analyzer or the like without performing a laborious operation of pretreatment such as centrifugation or electrophoresis. The method for eliminating triglycerides in lipoproteins other than low density lipoproteins includes allowing lipoprotein lipase, cholesterol esterase, glycerol kinase and glycerol-3-phosphate oxidase to act on a sample in the presence of a surfactant that acts on lipoproteins other than low density lipoprotein and/or a surfactant having LDL-protecting action, and eliminating hydrogen peroxide produced thereby.
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公开(公告)号：US20170146553A1
公开(公告)日：2017-05-25
申请号：US15401652
申请日：2017-01-09
发明人： Masanori HARA , Akihiko SAITO , Yasuhiko TOMINO , Katsuhiko ASANUMA , Hiroyuki KUROSAWA , Shinya OGASAWARA , Yoshiaki HIRAYAMA
IPC分类号： G01N33/68
CPC分类号： G01N33/6893 , A61K31/401 , A61K31/55 , G01N2800/347 , A61K2300/00
摘要： Provided is a test method for the assessment of the necessity of renal biopsy in a subject to be tested, who is suspected of having a renal disease. Specifically provided are a test method for a renal disease, including using urinary podocalyxin and one or more additional markers in combination, and a test reagent for use in the test method and a test reagent kit for use in the test method. The present invention allows the discrimination of a poor prognosis group even for poor prognosis cases with no overt findings in a conventional test method, and thus allows the assessment of a renal disease, the assessment of the necessity of renal biopsy, prognostic prediction, and the like to be performed exactly.
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7.发明授权公开(公告)号：US09625452B2
公开(公告)日：2017-04-18
申请号：US14768527
申请日：2014-02-19
发明人： Kunihiro Yamagata , Akira Hiwatashi , Masahiro Hagiwara , Hiroyuki Kurosawa , Yoshiaki Hirayama
IPC分类号： G01N33/53 , G01N33/566 , G01N33/493
CPC分类号： G01N33/5308 , G01N33/493 , G01N33/566 , G01N2333/47 , G01N2800/347
摘要： The object of the present invention is to provide a method for estimating the glomerular renal function in a convenient and non-invasive manner. As a result of intensive studies to achieve the above object, the present inventors found that there is a high correlation between the urinary megalin excretion rate and the estimated glomerular filtration rate (eGFR) in renal disease patients, and the glomerular filtration rate (GFR) can be estimated with high probability in a non-invasive manner by measuring the megalin level in the urine. This has led to the completion of the present invention.
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公开(公告)号：US09244072B2
公开(公告)日：2016-01-26
申请号：US14344784
申请日：2012-09-13
申请人： Motohiro Miki
发明人： Motohiro Miki
IPC分类号： G01N33/577 , C07K16/10 , G01N33/569
CPC分类号： G01N33/56983 , C07K16/10 , C07K2317/34 , C07K2317/55 , G01N2333/08
摘要： An anti-human-norovirus GII antibody which responds to substantially all genotypes of the human noroviruses belonging to GII and which can comprehensively detect such human noroviruses GII. The anti-human-norovirus GII antibody that binds to at least one of epitopes which are contained in amino acid regions represented by the following formulas (1) and (2): P-X1-X2-P-G-E (1) (SEQ ID NO: 2) X3-X4-X5-F-Y-X6-L-X7-P-X8 (2) (SEQ ID NO: 3) (wherein, X1 represents L, V, N, T, S, M, or R; X2 represents F, Y, or M; X3 represents V or G; X4 represents N or S; X5 represents Q, P, or S; X6 represents S, T, or I; X7 represents A or S; and X8 represents M or V), and of an epitope formed of amino acid 483 of the amino acid sequence represented by SEQ ID NO: 1, or an epitope formed of an amino acid corresponding to amino acid 483, the regions and the amino acids being present in the P domain of a capsid structural protein of a human norovirus GII.
摘要翻译： 抗人诺诺病毒GII抗体，其对于属于GII的人类诺维病毒的基本上所有基因型都有反应，并能够全面检测这种人类诺维病毒GII。 P-X1-X2-PGE（1）（SEQ ID NO：1）表示的氨基酸区域中含有的表位中的至少一个的抗人 - 诺如病毒GII抗体 ：2）X3-X4-X5-FY-X6-L-X7-P-X8（2）（SEQ ID NO：3）（其中，X1表示L，V，N，T，S，M或R; X2表示F，Y或M; X3表示V或G; X4表示N或S; X5表示Q，P或S; X6表示S，T或I; X7表示A或S; X8表示M或 V）以及由SEQ ID NO：1表示的氨基酸序列的氨基酸483形成的表位或由对应于氨基酸483的氨基酸形成的表位，所述区域和氨基酸存在于P 人类诺如病毒GII的衣壳结构蛋白的结构域。
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9.发明授权Method for quantifying the amount of cholesterol in high-density lipoprotein 3 有权用于定量高密度脂蛋白3中胆固醇含量的方法公开(公告)号：US09151768B2
公开(公告)日：2015-10-06
申请号：US13811569
申请日：2011-07-22
申请人： Maiko Higuchi , Yasuki Itoh
发明人： Maiko Higuchi , Yasuki Itoh
摘要： A method that enables quantification of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring a laborious operation is disclosed. The method for quantifying cholesterol in HDL3 comprises reacting, with a test sample, a surfactant that specifically reacts with high-density lipoprotein 3, and quantifying cholesterol. The method enables specific quantification of HDL3 cholesterol in a test sample using an automatic analyzer without requirement of a laborious operation such as ultracentrifugation or pretreatment. Further, quantification of the HDL2 cholesterol level can also be carried out by subtracting the HDL3 cholesterol level from the total HDL cholesterol level obtained by a conventional method for quantifying the total HDL cholesterol in a test sample.
摘要翻译： 公开了一种能够在测试样品中定量高密度脂蛋白3（HDL3）中的胆固醇而不需要费力操作的方法。 用于定量HDL3中的胆固醇的方法包括与测试样品与特异性地与高密度脂蛋白3反应的表面活性剂与量化胆固醇反应。 该方法可以使用自动分析仪在测试样品中进行HDL3胆固醇的特异性定量，而无需费力操作，如超速离心或预处理。 此外，HDL2胆固醇水平的定量也可以通过从测定样品中用于定量总HDL胆固醇的常规方法获得的总HDL胆固醇水平中减去HDL3胆固醇水平来进行。
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10.发明授权Method and kit for quantitative determination for small, dense particle low density lipoproteins 有权用于定量测定小，致密颗粒低密度脂蛋白的方法和试剂盒公开(公告)号：US08697378B2
公开(公告)日：2014-04-15
申请号：US12065125
申请日：2006-08-31
申请人： Yasuki Itoh
发明人： Yasuki Itoh
IPC分类号： C12Q1/60
摘要： A rapid and convenient method capable of performing fractional measurement of small, dense LDLs without pretreatment of a specimen, which is adaptable for an autoanalyzer, is provided. A method for quantitatively determining small, dense LDL cholesterol is provided, which comprises adding enzymes for cholesterol measurement to a test sample in the presence of a polyoxyethylene-polyoxypropylene copolymer or a derivative thereof, causing the polyoxyethylene-polyoxypropylene copolymer or the derivative thereof to selectively act on small, dense LDLs among lipoproteins, and then measuring the amount of cholesterol generated.
摘要翻译： 提供了一种快速方便的方法，能够对小而密的低密度脂蛋白进行分数测量，而无需预处理样品，适用于自动分析仪。 提供了一种定量测定小而密LDL胆固醇的方法，其中包括在聚氧乙烯 - 聚氧丙烯共聚物或其衍生物存在下，向测试样品中加入胆固醇测定酶，使聚氧乙烯 - 聚氧丙烯共聚物或其衍生物选择性地 作用于脂蛋白中的小而密LDL，然后测量产生的胆固醇的量。
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