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公开(公告)号：US09657343B2
公开(公告)日：2017-05-23
申请号：US14070904
申请日：2013-11-04
申请人： Eve Biomedical, Inc.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2521/119 , C12Q2521/543 , C12Q2523/303 , C12Q2563/143 , C12Q2565/601
摘要： Provided herein are rotation-dependent transcriptional sequencing methods and systems.
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2.发明申请公开(公告)号：US20140162275A1
公开(公告)日：2014-06-12
申请号：US14070904
申请日：2013-11-04
申请人： Eve Biomedical, Inc.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2521/119 , C12Q2521/543 , C12Q2523/303 , C12Q2563/143 , C12Q2565/601
摘要： Provided herein are rotation-dependent transcriptional sequencing methods and systems.
摘要翻译： 本文提供了旋转依赖性转录测序方法和系统。
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3.发明授权公开(公告)号：US08574840B2
公开(公告)日：2013-11-05
申请号：US13403645
申请日：2012-02-23
CPC分类号： C12Q1/6869 , C12Q1/6874 , C12Q2521/119 , C12Q2521/543 , C12Q2523/303 , C12Q2563/143 , C12Q2565/601
摘要： Provided herein are rotation-dependent transcriptional sequencing methods and systems.
摘要翻译： 本文提供了旋转依赖性转录测序方法和系统。
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公开(公告)号：US09725763B2
公开(公告)日：2017-08-08
申请号：US14185469
申请日：2014-02-20
申请人： Eve Biomedical, Inc.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2521/119 , C12Q2521/543 , C12Q2563/143 , C12Q2565/631 , C12Q2521/101
摘要： Provided herein are nanostructure-based sequencing methods and systems. Such methods and systems include contacting an immobilized RNA polymerase with a double-stranded target nucleic acid molecule under sequencing conditions, where the sequencing conditions include the presence of four nucleoside triphosphates, where one of the nucleoside triphosphates is present in a rate-limiting amount; detecting the movement of the target nucleic acid molecule and/or one or more nascent strand(s) through, on or over a nanostructure; repeating the contacting and detecting steps a plurality of times; and determining the sequence of the target nucleic acid molecule based, sequentially, on the presence or absence of a change in the movement in the presence of the at least one nucleoside triphosphate.
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