公开(公告)号：US11530442B2

公开(公告)日：2022-12-20

申请号：US16418104

申请日：2019-05-21

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/6869 ,  C12Q1/6806

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US11519028B2

公开(公告)日：2022-12-06

申请号：US17494726

申请日：2021-10-05

申请人： Natera, Inc.

发明人： Bernhard Zimmermann ,  Ryan Swenerton ,  Matthew Rabinowitz ,  Styrmir Sigurjonsson ,  George Gemelos ,  Apratim Ganguly ,  Himanshu Sethi

IPC分类号： C12Q1/6806 ,  C12Q1/6869

摘要： The present disclosure provides methods and compositions for sequencing nucleic acid molecules and identifying individual sample nucleic acid molecules using Molecular Index Tags (MITs). Furthermore, reaction mixtures, kits, and adapter libraries are provided.

公开(公告)号：US11414709B2

公开(公告)日：2022-08-16

申请号：US16359917

申请日：2019-03-20

申请人： Natera, Inc.

发明人： Joshua Babiarz ,  Tudor Pompiliu Constantin ,  Lane A. Eubank ,  George Gemelos ,  Matthew Micah Hill ,  Huseyin Eser Kirkizlar ,  Matthew Rabinowitz ,  Onur Sakarya ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann

IPC分类号： C12Q1/6886 ,  C12Q1/6869 ,  G06N20/00 ,  G16B15/00 ,  G16B25/00 ,  G16B40/00 ,  G16B40/20 ,  G16B20/10 ,  G16B20/20 ,  G16H50/20 ,  G06N7/00 ,  G16H10/40 ,  G16Z99/00 ,  G16B20/00 ,  G16B25/20

摘要： The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

公开(公告)号：US11339429B2

公开(公告)日：2022-05-24

申请号：US16411770

申请日：2019-05-14

申请人： Natera, Inc.

发明人： Matthew Rabinowitz ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Zachary Demko ,  Matthew Hill ,  Bernhard Zimmermann ,  Johan Baner ,  Styrmir Sigurjonsson

IPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6862 ,  G16B20/00 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q1/6827 ,  G16B20/10 ,  G16B40/00

摘要： The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

公开(公告)号：US11326208B2

公开(公告)日：2022-05-10

申请号：US17320540

申请日：2021-05-14

申请人： Natera, Inc.

发明人： Matthew Rabinowitz ,  Matthew Hill ,  Bernhard Zimmermann ,  Johan Baner ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Styrmir Sigurjonsson ,  Zachary Demko

IPC分类号： C12Q1/6869 ,  G16B10/00 ,  C12Q1/6876 ,  C12Q1/6844

摘要： Methods for non-invasive prenatal paternity testing are disclosed herein. The method uses genetic measurements made on plasma taken from a pregnant mother, along with genetic measurements of the alleged father, and genetic measurements of the mother, to determine whether or not the alleged father is the biological father of the fetus. This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.

公开(公告)号：US11322224B2

公开(公告)日：2022-05-03

申请号：US14532666

申请日：2014-11-04

申请人： Natera, Inc.

发明人： Matthew Rabinowitz ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Zachary Demko ,  Matthew Hill ,  Bernhard Zimmermann ,  Johan Baner

IPC分类号： C12Q1/68 ,  G16B20/00 ,  C12Q1/6827 ,  C12Q1/6862 ,  C12Q1/686 ,  G16B20/10 ,  G16B20/20 ,  G16B20/40 ,  C12Q1/6883 ,  C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6874 ,  G16B40/00

摘要： The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.

公开(公告)号：US11319596B2

公开(公告)日：2022-05-03

申请号：US16288351

申请日：2019-02-28

申请人： Natera, Inc.

发明人： Joshua Babiarz ,  Tudor Pompiliu Constantin ,  Lane A. Eubank ,  George Gemelos ,  Matthew Micah Hill ,  Huseyin Eser Kirkizlar ,  Matthew Rabinowitz ,  Onur Sakarya ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann

IPC分类号： C12Q1/6886 ,  C12Q1/6869 ,  G16B20/20 ,  G06N20/00 ,  G16B15/00 ,  G16B25/00 ,  G16B40/00 ,  G16B40/20 ,  G16B20/10 ,  G16B20/00 ,  G16Z99/00 ,  G16H50/20 ,  G06N7/00 ,  G16H10/40 ,  G16B25/20

摘要： The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

公开(公告)号：US11319595B2

公开(公告)日：2022-05-03

申请号：US15898145

申请日：2018-02-15

申请人： Natera, Inc.

发明人： Joshua Babiarz ,  Tudor Pompiliu Constantin ,  Lane A. Eubank ,  George Gemelos ,  Matthew Micah Hill ,  Huseyin Eser Kirkizlar ,  Matthew Rabinowitz ,  Onur Sakarya ,  Styrmir Sigurjonsson ,  Bernhard Zimmermann

IPC分类号： C12Q1/6886 ,  G06N20/00 ,  G16B15/00 ,  G16B25/00 ,  G16B40/00 ,  C12Q1/6869 ,  G16B40/20 ,  G16B20/10 ,  G16B20/00 ,  G16B20/20 ,  G16Z99/00 ,  G16H50/20 ,  G06N7/00 ,  G16H10/40 ,  G16B25/20

摘要： The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

公开(公告)号：US11286530B2

公开(公告)日：2022-03-29

申请号：US17196822

申请日：2021-03-09

申请人： Natera, Inc.

发明人： Matthew Rabinowitz ,  Matthew Micah Hill ,  Bernhard Zimmermann ,  Johan Baner ,  George Gemelos ,  Milena Banjevic ,  Allison Ryan ,  Styrmir Sigurjonsson ,  Zachary Demko

IPC分类号： C12Q1/68 ,  C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6811 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6848

摘要： The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

公开(公告)号：US20220056534A1

公开(公告)日：2022-02-24

申请号：US17413464

申请日：2019-12-16

申请人： Natera, Inc.

发明人： Elizabeth RIVERS ,  Maxim BREVNOV, Ph.D.

IPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6869

摘要： The invention provides methods for characterizing and analyzing circulating cells. In particular, the invention provides methods for confirming the identity of an individual cell and that the obtained sample was derived from a single cell of a defined identity. Additional methods are provided for analyzing single cell samples to determine copy number variation and aneuploidy in the context of circulating fetal cells, microdeletions, single nucleic acid variations associated with cancer, or early relapse of cancer and metastasis.