Process for the enzymatic synthesis and amplification of nucleic acids

    公开(公告)号:US11203780B2

    公开(公告)日:2021-12-21

    申请号:US16796109

    申请日:2020-02-20

    申请人: AGCT GmbH

    摘要: A method for amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results. Amplification of a particular target sequence takes place more efficiently in case of perfect match complementary base pair formation between the activator oligonucleotide and the corresponding target sequence. Mismatches between the activator oligonucleotide and a particular target sequence can result in less efficient amplification. The efficiency of synthesis of perfect match target sequences and mismatch sequences can be measured and compared.

    Polyamide foams which inhibit the spread of fires for filling cavities in mining

    公开(公告)号:US11130834B2

    公开(公告)日:2021-09-28

    申请号:US16326869

    申请日:2017-09-04

    摘要: Polyamide foams which do not propagate fire are obtained by mixing (i) a liquid isocyanate component which comprises at least one polyisocyanate and in which the molar ratio of aromatic isocyanate groups to the sum of aromatic and aliphatic isocyanate groups is at least 60 mol %, with (ii) at least one liquid isocyanatereactive component which comprises a reactive diluent, and the reactive diluent 10 comprises (a) a chain-extending and/or crosslinking reactive diluent selected from among aliphatic branched C24-66-polycarboxylic acids, alicyclic C24-66-polycarboxylic acids and partial esters of polycarboxylic acids having at least two unesterified carboxyl groups and/or (b) a chain-terminating reactive diluent selected from among aliphatic branched C24-66-monocarboxylic acids, alicyclic C24-66-monocarboxylic acids and partial esters of polycarboxylic acids having one unesterified carboxyl group, wherein the liquid isocyanate-reactive component comprises an aromatic C8-18-polycarboxylic acid and/or an anhydride thereof.

    Encapsulation
    5.
    发明授权

    公开(公告)号:US11102991B2

    公开(公告)日:2021-08-31

    申请号:US15025684

    申请日:2014-10-29

    申请人: GIVAUDAN SA

    发明人: Frans Witteveen

    摘要: An encapsulated flavour, comprising a core material, flavour material and a coating material, the core material comprising a finely-divided native starch, xanthan gum and konjac. The encapsulated flavours may be completely gelatin-free, while retaining the desirable qualities of gelatin.

    Organic compounds
    6.
    发明授权

    公开(公告)号:US11098266B2

    公开(公告)日:2021-08-24

    申请号:US16767644

    申请日:2018-12-05

    申请人: Givaudan SA

    IPC分类号: C11B9/00

    摘要: The present invention refers to 3,7-dimethyloct-6-en-1-yl methyl carbonate and its use as fragrance ingredient. The invention further refers to fragrance compositions and fragranced articles including it.