公开(公告)号：US12098352B2

公开(公告)日：2024-09-24

申请号：US16983850

申请日：2020-08-03

申请人： EMULATE, INC.

发明人： Janna Nawroth ,  Riccardo Barrile ,  David Conegliano ,  Remi Villenave ,  Carolina Lucchesi ,  Justin Nguyen ,  Antonio Varone ,  Catherine Karalis ,  Geraldine Hamilton

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12N5/071 ,  C12N5/074

CPC分类号： C12M23/16 ,  B01L3/5027 ,  C12N5/0688 ,  C12N5/0696 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2502/1323 ,  C12N2502/27 ,  C12N2503/04 ,  C12N2506/02 ,  C12N2513/00 ,  G01N2800/12

摘要： An in vitro microfluidic “organ-on-chip” device is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a stem cell-based Lung-on-Chip is described. This in vitro microfluidic system can be used for modeling differentiation of cells on-chip into lung cells, e.g., a lung (Lung-On-Chip), bronchial (Airway-On-Chip; small-Airway-On-Chip), alveolar sac (Alveolar-On-Chip), etc., for use in modeling disease states of derived tissue, i.e. as healthy, pre-disease and diseased tissues. Additionally, stem cells under differentiation protocols for deriving (producing) differentiated lung cells off-chips may be seeded onto microfluidic devices at any desired point during the in vitro differentiation pathway for further differentiation on-chip or placed on-chip before, during or after terminal differentiation.

公开(公告)号：US12018284B2

公开(公告)日：2024-06-25

申请号：US17984023

申请日：2022-11-09

申请人： EMULATE, INC.

发明人： Athanasia Apostolou ,  Antonio Varone ,  Magdalena Kasendra ,  Raymond Luc

IPC分类号： C12N5/071 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M3/06 ,  G01N1/30 ,  G01N33/50

CPC分类号： C12N5/0679 ,  B01L3/502715 ,  B01L3/502761 ,  C12M23/16 ,  C12M23/26 ,  C12M25/02 ,  C12N5/069 ,  G01N1/30 ,  G01N33/5047 ,  G01N33/5064 ,  B01L2200/16 ,  B01L2300/123 ,  B01L2300/16 ,  C12N2500/00 ,  C12N2501/052 ,  C12N2501/2301 ,  C12N2501/2306 ,  C12N2501/25

摘要： An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

公开(公告)号：US12018100B2

公开(公告)日：2024-06-25

申请号：US17752510

申请日：2022-05-24

申请人： RIMFROST TECHNOLOGIES AS

发明人： Inge Bruheim

IPC分类号： C08B37/00 ,  C08B37/08 ,  C12P21/06

CPC分类号： C08B37/0003 ,  C08B37/003 ,  C12P21/06

摘要： Methods of producing a chitin composition by which the properties of the chitin composition derived from krill, such as molecular weight (chain length) and degree of acetylation are reproducible, are claimed. Such reproducible production methods allow for a wide use of the chitin composition end products in pharmaceutical and biomedical applications. The methods also allow for the production of chitin compositions which preserve the native state of chitin.

公开(公告)号：US11999932B2

公开(公告)日：2024-06-04

申请号：US17036652

申请日：2020-09-29

申请人： EMULATE, Inc.

发明人： Christopher David Hinojosa ,  Grace Ahn

IPC分类号： C12M3/06 ,  C12M1/00 ,  C12M1/02 ,  C12M1/12

CPC分类号： C12M23/16 ,  C12M23/26 ,  C12M25/02 ,  C12M29/00 ,  C12M41/18

摘要： The present invention is related to the field of fluidic devices, and in particular, microfluidic cell culture systems. The present invention provides pumping, recirculation and sampling for a microfluidic device or devices. The present invention provides solutions to the control of microfluidics for both terrestrial and space applications, including the control over the movement of fluids in zero gravity or microgravity.

公开(公告)号：US11970680B2

公开(公告)日：2024-04-30

申请号：US16089220

申请日：2017-03-30

申请人： EMULATE, INC.

发明人： Geraldine A. Hamilton ,  Norman Wen ,  Catherine Karalis ,  Antonio Varone ,  Daniel Levner ,  Riccardo Barrile

IPC分类号： G01N31/00 ,  C12M1/12 ,  C12M3/06 ,  C12N5/09 ,  G01N33/50 ,  G01N33/53

CPC分类号： C12M23/16 ,  C12M25/02 ,  C12N5/0693 ,  G01N33/5011 ,  G01N33/5017

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.

公开(公告)号：US11859165B2

公开(公告)日：2024-01-02

申请号：US18097755

申请日：2023-01-17

申请人： EMULATE, INC.

发明人： Daniel Levner ,  Christopher David Hinojosa ,  Norman Wen ,  Antonio Varone ,  Justin Nguyen ,  Lina Williamson ,  S. Jordan Kerns ,  Catherine Karalis ,  Geraldine Hamilton ,  Carol Lucchesi

IPC分类号： C12M3/06 ,  C12M1/00 ,  C12M1/12 ,  C12M1/42 ,  G01N33/50 ,  G01N33/543 ,  C12N5/0793 ,  C12N5/079 ,  C12N5/071 ,  C12N5/077

CPC分类号： C12M35/04 ,  C12M23/16 ,  C12M23/38 ,  C12M25/02 ,  C12M35/08 ,  C12N5/069 ,  C12N5/0619 ,  C12N5/0622 ,  C12N5/0629 ,  C12N5/0656 ,  C12N5/0688 ,  C12N5/0697 ,  C12N5/0698 ,  G01N33/50 ,  G01N33/5082 ,  G01N33/54366 ,  C12N2501/165

摘要： A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

公开(公告)号：US11852781B2

公开(公告)日：2023-12-26

申请号：US17374404

申请日：2021-07-13

申请人： The Trustees of Princeton University

发明人： Paul J Steinhardt ,  Marian Florescu ,  Salvatore Torquato

IPC分类号： G02B1/00 ,  G02B5/30 ,  G02B6/125 ,  B82Y20/00 ,  G01K17/00 ,  G01L1/24 ,  G01N21/17 ,  H01L33/00 ,  H01L33/16 ,  G02B6/122 ,  G02B27/00

CPC分类号： G02B1/005 ,  B82Y20/00 ,  G01K17/00 ,  G01L1/24 ,  G01N21/17 ,  G02B5/3025 ,  G02B6/125 ,  G02B6/1225 ,  G02B27/0012 ,  H01L33/0058 ,  H01L33/0095 ,  H01L33/16 ,  Y10T29/49993

摘要： Waveguides and electromagnetic cavities fabricated in hyperuniform disordered materials with complete photonic bandgaps are provided. Devices comprising electromagnetic cavities fabricated in hyperuniform disordered materials with complete photonic bandgaps are provided. Devices comprising waveguides fabricated in hyperuniform disordered materials with complete photonic bandgaps are provided. The devices include electromagnetic splitters, filters, and sensors.

公开(公告)号：US11834641B2

公开(公告)日：2023-12-05

申请号：US17950422

申请日：2022-09-22

申请人： EMULATE, Inc

发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Joshua Gomes ,  Jose Fernandez-Alcon

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  C12N5/071 ,  A01N1/02 ,  C12M3/00 ,  C12M1/36 ,  B01L9/00

CPC分类号： C12M23/16 ,  A01N1/021 ,  A01N1/0247 ,  B01L3/50273 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L9/527 ,  C12M21/08 ,  C12M23/38 ,  C12M23/40 ,  C12M23/42 ,  C12M29/10 ,  C12M35/04 ,  C12M41/40 ,  C12M41/48 ,  C12N5/0602 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  C12N2521/00

摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

公开(公告)号：US11793873B2

公开(公告)日：2023-10-24

申请号：US16612152

申请日：2018-05-01

申请人： UNIVERSITY OF MASSACHUSETTS

发明人： Daniel H. Libraty

IPC分类号： A61K39/00 ,  A61K39/295 ,  A61K47/65 ,  A61P31/20 ,  A61P31/14 ,  A61K39/12 ,  A61K39/29

CPC分类号： A61K39/295 ,  A61K39/12 ,  A61K39/292 ,  A61K47/65 ,  A61P31/14 ,  A61P31/20

摘要： The present invention relates to the construction of, and immunization with viral vaccines. In particular, bivalent vaccines that are capable of providing simultaneous virus infection protection for two or more different viruses. Furthermore, the bivalent vaccines contemplated herein are contemplated as being effective in a neonatal mammal. One such bivalent viral vaccine comprises two antigenic epitopes against the dengue viruses and at least one antigenic epitope against hepatitis B virus. Immunization cross-reactivity may also provide infection protection against other viruses as well.

公开(公告)号：US11789027B2

公开(公告)日：2023-10-17

申请号：US17399461

申请日：2021-08-11

申请人： AmberGen, Inc.

发明人： Mark J. Lim ,  Gargey Yagnik ,  Kenneth J. Rothschild

IPC分类号： C07C271/20 ,  G01N33/68 ,  C12Q1/6841

CPC分类号： G01N33/6851 ,  C07C271/20 ,  C12Q1/6841 ,  G01N2400/00 ,  G01N2458/15

摘要： The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. The field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout, and also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI.