摘要:
Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.
摘要:
This invention is a sound apparatus having a plurality of different kinds of speakers and that independently performs correction of the sound signal for each speaker to obtain optimum sound and sound field. This sound apparatus has an output device that receives audio signals and outputs sound, and comprises: a correction device for correcting the audio signals that are input to each of the output devices; and a correction-characteristic-setting device which sets correction characteristic for each of the output devices; and where the correction device correct the audio signals based on the set correction characteristic.
摘要:
The present invention relates to a production method of an enantiomer-enriched compound, which includes the following (1) and (2): (1) contacting an enantiomer mixture with a stereoselective dehydrogenase, for which NAD+ (nicotinamide adenine dinucleotide) is a coenzyme, thereby preferentially oxidizing one enantiomer and leaving the other enantiomer to form NADH, and (2) contacting the NADH formed by the oxidation reaction in (1) with a water-forming NADH oxidase stable under oxidative conditions to convert the NADH to NAD+. According to the present invention, in the production of an enantiomer-enriched compound from an enantiomer mixture, which uses a dehydrogenase, NAD+ can be regenerated even without addition of a stabilizer and an enantiomer-enriched compound can be obtained efficiently.
摘要:
The present invention provides an industrially advantageous process for biochemical production of glyoxylic acid from glyoxal. More specifically, the present invention provides a process for production of glyoxylic acid, which is characterized in that the process comprises allowing oxidoreductase that can convert glyoxal into glyoxylic acid, such as oxidase and dehydrogenase, to act on glyoxal, so as to convert glyoxal into glyoxylic acid.
摘要:
An impact energy absorbing mechanism for a steering device of a motor vehicle includes guides each having a generally U-shaped guide surface. Each guide is mounted to a steering column which is movable in the axial direction when the operator's body is thrown out onto a steering wheel due a collision of the vehicle. A retaining portion is provided integrally with the guide and at a predetermined distance from the guide surface. An absorbing plate is fixed at one end to the vehicle body and left free at the other end. The free end of the absorbing plate is inserted and retained in a gap defined between the guide surface and retaining portion of the guide, so that the absorbing plate is held in a generally U-shaped bent position along the guide surface. When the guide is moved together with the steering column, the bent portion of the absorbing plate is sequentially shifted while maintaining the shape which is defined by the guide, thereby absorbing impact energy.
摘要:
An aerosol sprayer, which comprises a can for accommodating an aerosol material which is provided with a spray nozzle and connected with a pressure regulator, and a small-sized bomb which is charged with a highly compressed gas and connected with said pressure regulator.
摘要:
The method for preparing an optically active (R)-amino compound characterized by the method comprising stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor), and an amino compound (amino donor) of a racemic form or an (R)-form, to give an optically active (R)-amino compound. According to the present invention, it is made possible to easily prepare at a high yield the optically active (R)-amino compounds and the like having an aryl group and the like at their 1-position, which have been conventionally difficult to prepare.
摘要:
Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.
摘要:
A pair of compression rollers 38a and 38b parallel with each other are provided and powder grains are supplied to a powder grain introduction/compression part 50 formed between the rollers 38a and 38b and, thereby, compression moldings of the powder grains are formed. A powder grain press/feed means 20 is provided in a front stage of the rollers 38a and 38b. The press/feed means 20 has a deaerating barrel 24 and previously presses the powder grains supplied between the rollers 38a and 38b. In side surfaces of the rollers 38a and 38b, side seals 37 are arranged with clearances 72 maintained from the rollers 38a and 38b. During pressing the powder moldings, the powder grains enter into the clearances so that closer layers are formed between the side surfaces of the compression rollers 38a and 38b and the side seals 37, thereby sealing the powder grain introduction/compression part 50. In a rear stage of the rollers 38a and 38b, a shearing device 75 is provided and shears the compression moldings formed by the rollers 38a and 38b. Torque of the shearing device 75 is detected by a torque sensor, and the compression rollers 38a and 38b and the powder grain transport means 17 are controlled in accordance with the detected torque.
摘要:
The invention provides an alkali-soluble adhesive agent characterized by comprising an alkali-soluble resin that is a reaction product (C) obtained by allowing a polycarboxylic acid and/or a polycarboxylic anhydride having two or more carboxyl groups to react with a reaction product of a compound (A) having at least one epoxy group with a monocarboxylic acid compound (B). Preferably, the alkali-soluble resin is used in combination with a ketone organic solvent, optionally with at least one member selected from the group consisting of a melt viscosity reducing agent, a surface active agent and a plasticizer. The alkali-soluble adhesive agent can be removed by an alkali aqueous solution without recourse to any organic solvent, and is of an environmental protection-conscious type with improvements in adhesion, adhesion strength, coatability, surface smoothness, softening point controllability, melt viscosity controllability and spread controllability.