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公开(公告)号:US06858234B2
公开(公告)日:2005-02-22
申请号:US10349714
申请日:2003-01-23
申请人: Ryuji Murayama , Takeo Kanafuji , Yasuhito Muranaka , Rumiko Muranaka , Kazuo Sato , Akira Sekigawa , Kazuhiko Yamada , Yoshio Suzuki , Hiroyuki Ikemoto
发明人: Ryuji Murayama , Takeo Kanafuji , Yasuhito Muranaka , Rumiko Muranaka , Kazuo Sato , Akira Sekigawa , Kazuhiko Yamada , Yoshio Suzuki , Hiroyuki Ikemoto
IPC分类号: A23J1/12 , A61K36/899 , A61K38/00 , A61K38/55 , C07K14/415 , C12N9/26 , C12N9/99 , A61K35/78
CPC分类号: A61K36/899
摘要: The process for the preparation of amylase inhibitor including the steps of: (A) obtaining an extract solution containing the amylase inhibitor; (B) insolubilizing the amylase inhibitor by salting out by addition of a salt or salts to the solution obtained in the step (A) and recovering the insolubilized substance resulting from the salting out; and (C) directly drying the insolubilized substance recovered in the step (B) or dissolving the insolubilized substance in water to prepare an aqueous solution, and desalting and drying the aqueous solution to recover the amylase inhibitor, whereby, an amylase inhibitor in high concentrations 0.19 AI is prepared that shows highly inhibitive activity against the amylase in high yield and with good productivity.
摘要翻译: 制备淀粉酶抑制剂的方法,包括以下步骤:(A)获得含有淀粉酶抑制剂的提取液; (B)通过向步骤(A)中得到的溶液中加入盐或盐盐析而使淀粉酶抑制剂不溶解并回收由盐析产生的不溶物质; 和(C)直接干燥步骤(B)中回收的不溶物质或将不溶物质溶解在水中以制备水溶液,并对该水溶液进行脱盐干燥以回收淀粉酶抑制剂,从而得到高浓度的淀粉酶抑制剂 制备了以高产率和高生产率对淀粉酶具有高度抑制活性的AII。