公开(公告)号：US20130288272A1

公开(公告)日：2013-10-31

申请号：US13823922

申请日：2011-09-09

申请人： Hisashi Narimatsu ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Hideki Matsuzaki ,  Yoshitoshi Hirao ,  Jun Iwaki ,  Minako Abe ,  Masaharu Nomura ,  Masayuki Noguchi

发明人： Hisashi Narimatsu ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Hideki Matsuzaki ,  Yoshitoshi Hirao ,  Jun Iwaki ,  Minako Abe ,  Masaharu Nomura ,  Masayuki Noguchi

IPC分类号： G01N33/68

CPC分类号： G01N33/6893 ,  C07K14/4748 ,  C07K16/28 ,  C07K16/3023 ,  G01N33/574 ,  G01N33/57423 ,  G01N2440/38

摘要： An object of the present invention is to develop and provide a lung cancer differential marker with which lung cancer can be diagnosed conveniently and highly sensitively without depending only on increase or decrease in protein expression level between cancer patients and healthy persons. Another object of the present invention is to develop and provide a glycan marker capable of distinguishing histological types of lung cancer. Of serum glycoproteins, glycopeptide and glycoprotein groups whose glycan structures were altered specifically in lung cancer cell culture supernatants were identified, and they are provided as lung cancer differential markers.

摘要翻译： 本发明的目的是开发和提供肺癌鉴别标记物，其可以方便和高度灵敏地诊断肺癌，而不依赖于癌症患者和健康人之间的蛋白质表达水平的增加或降低。 本发明的另一个目的是开发和提供能够区分肺癌的组织学类型的聚糖标记物。 确定了在肺癌细胞培养上清液中特异性改变其聚糖结构的血清糖蛋白，糖肽和糖蛋白，并将其作为肺癌鉴别标记。

公开(公告)号：US20100279355A1

公开(公告)日：2010-11-04

申请号：US12772117

申请日：2010-04-30

申请人： Hisashi NARIMATSU ,  Akira Togayachi ,  Niro Inaba ,  Toru Hiruma ,  Yasuko Ishizuka

发明人： Hisashi NARIMATSU ,  Akira Togayachi ,  Niro Inaba ,  Toru Hiruma ,  Yasuko Ishizuka

IPC分类号： C12P19/46 ,  C12P19/26 ,  C12P19/30

CPC分类号： C12N9/1051

摘要： The N-acetyl-D-galactosamine transferase protein of the present invention is characterized by transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage, and it preferably has the amino acid sequence shown in SEQ ID NO: 2 or 4. The canceration assay according to the present invention uses a nucleic acid for measurement which hybridizes under stringent conditions to the nucleotide sequence shown in SEQ ID NO: 1 or 3 or a nucleotide sequence complementary to at least one of them.

摘要翻译： 本发明的N-乙酰基-D-半乳糖胺转移酶蛋白的特征在于将N-乙酰基-D-半乳糖胺转移至具有1,3-连接的N-乙酰基-D-葡糖胺，并且其优选具有所示的氨基酸序列 根据本发明的癌化测定使用在严格条件下与SEQ ID NO：1或3所示的核苷酸序列杂交的核酸或与至少一个氨基酸互补的核苷酸序列的核酸 的他们。

公开(公告)号：US20090081668A1

公开(公告)日：2009-03-26

申请号：US12155320

申请日：2008-06-02

申请人： Hisashi Narimatsu ,  Takashi Kudo ,  Akira Togayachi ,  Toru Hiruma

发明人： Hisashi Narimatsu ,  Takashi Kudo ,  Akira Togayachi ,  Toru Hiruma

IPC分类号： C12Q1/68 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C12P21/04 ,  C07K16/18

CPC分类号： C12N9/1051

摘要： A tumor marker nucleic acid of the present invention is concerned with a nucleic acid hybridizing under stringent conditions to a nucleotide sequence described in SEQ ID NO: 1 or a complementary nucleotide sequence thereof. A method of testing canceration of the present invention is a method comprising diagnosing a biological sample as being cancerous when the transcription level of the nucleic acid in the biological sample significantly exceeds that in a normal biological sample as a control. The present invention also relates to a β1,3-N-acetyl-D-glucosaminyltransferase protein having an activity of transferring N-acetyl-D-glucosamine from a donor substrate to an acceptor substrate through β1,3-linkage.

摘要翻译： 本发明的肿瘤标志物核酸涉及在严格条件下与SEQ ID NO：1所述核苷酸序列或其互补核苷酸序列杂交的核酸。 测定本发明的癌症的方法是当生物样品中的核酸的转录水平显着超过作为对照的正常生物样品中的生物样品时，将生物样品诊断为癌变的方法。 本发明还涉及具有通过β1,3键将N-乙酰基-D-葡糖胺从供体底物转移到受体底物的活性的β1,3-N-乙酰基-D-葡糖胺基转移酶蛋白。

公开(公告)号：US07241605B1

公开(公告)日：2007-07-10

申请号：US09914152

申请日：2000-02-24

申请人： Hisashi Narimatsu ,  Soichiro Isshiki ,  Akira Togayachi ,  Katsutoshi Sasaki

发明人： Hisashi Narimatsu ,  Soichiro Isshiki ,  Akira Togayachi ,  Katsutoshi Sasaki

IPC分类号： C12N9/10

CPC分类号： C12N9/1051 ,  A61K38/00 ,  A61K39/00 ,  C12P19/04

摘要： According to the present invention, there can be provided a novel polypeptide having β1,3-galactosyltransferase activity involved in the synthesis of type 1 sugar chains, a DNA coding for said polypeptide, a recombinant vector comprising said DNA, a transformant carrying said recombinant vector, a process for producing type 1 sugar chain-containing sugar chains and complex carbohydrates by use of said polypeptide or said transformant, an antibody recognizing said polypeptide, a method for detecting or quantifying said polypeptide by use of said antibody, a method for screening a substance correlated with said polypeptide, a method for diagnosis or treatment of cancers in the digestive system by use of said DNA or said antibody, and a method for treatment of cancers in the digestive system by use of a substance obtained by said screening method.

摘要翻译： 根据本发明，可以提供一种具有参与1型糖链合成的β1,3-半乳糖基转移酶活性的新型多肽，编码所述多肽的DNA，包含所述DNA的重组载体，携带所述重组载体的转化体 ，通过使用所述多肽或所述转化体产生含有1号糖链的糖链和复合碳水化合物的方法，识别所述多​​肽的抗体，通过使用所述抗体检测或定量所述多肽的方法，筛选 与所述多肽相关的物质，通过使用所述DNA或所述抗体在消化系统中诊断或治疗癌症的方法，以及通过使用通过所述筛选方法获得的物质来治疗消化系统中的癌症的方法。

公开(公告)号：US10247733B2

公开(公告)日：2019-04-02

申请号：US14234871

申请日：2012-07-26

申请人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

发明人： Maki Sogabe ,  Tomomi Kubota ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hiromichi Sawaki ,  Hayao Nakanishi ,  Toru Nakanishi

IPC分类号： G01N33/50 ,  G01N33/574 ,  C07K16/40

摘要： It is intended to find a highly specific epithelial ovarian cancer marker and to provide an antibody capable of specifically recognizing and detecting the marker or a fragment of the antibody. The present invention provides an anti-β1,6-N-acetylglucosaminyltransferase 5B antibody for diagnosis of epithelial ovarian cancer, i.e., an antibody for detection of a glycosyltransferase β1,6-N-acetylglucosaminyltransferase 5B as an epithelial ovarian cancer marker. The antibody recognizes, as an epitope, a part of a polypeptide of the enzyme consisting of the amino acid sequence represented by SEQ ID NO: 1.

公开(公告)号：US09796761B2

公开(公告)日：2017-10-24

申请号：US13384031

申请日：2010-07-12

申请人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

发明人： Hisashi Narimatsu ,  Jun Hirabayashi ,  Yuzuru Ikehara ,  Takashi Angata ,  Hiroyuki Kaji ,  Atsushi Kuno ,  Takashi Ohkura ,  Toshihide Shikanai ,  Maki Sogabe ,  Akira Togayachi ,  Makoto Ochou ,  Yasuhito Tanaka ,  Masashi Mizokami

IPC分类号： C40B30/04 ,  C07K14/00 ,  C07K9/00 ,  C07K14/47 ,  G01N33/574

CPC分类号： C07K9/00 ,  C07K14/47 ,  G01N33/57438 ,  G01N33/57469 ,  G01N2400/02 ,  G01N2800/085

摘要： The present invention is directed to developing a glycan markers capable of detecting a hepatic disease, and more specifically to developing a glycan marker indicating a hepatic disease-state. Furthermore, the present invention is also directed to developing a glycan marker capable of distinguishing hepatic disease-states with the progress of hepatocarcinoma. The present inventors identified, among the serum glycoproteins, glycopeptides and glycoproteins in which a glycan structure specifically changes due to a hepatic diseases including hepatocarcinoma and provide these as novel glycan markers (glycopeptide and glycoprotein) specific to hepatic disease-states.

公开(公告)号：US20130004995A1

公开(公告)日：2013-01-03

申请号：US13539290

申请日：2012-06-29

申请人： Hisashi NARIMATSU ,  Akira Togayachi ,  Niro Inaba ,  Toru Hiruma ,  Yasuko Ishizuka

发明人： Hisashi NARIMATSU ,  Akira Togayachi ,  Niro Inaba ,  Toru Hiruma ,  Yasuko Ishizuka

IPC分类号： C12P19/26

CPC分类号： C12N9/1051

摘要： The N-acetyl-D-galactosamine transferase protein of the present invention is characterized by transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage, and it preferably has the amino acid sequence shown in SEQ ID NO: 2 or 4. The canceration assay according to the present invention uses a nucleic acid for measurement which hybridizes under stringent conditions to the nucleotide sequence shown in SEQ ID NO: 1 or 3 or a nucleotide sequence complementary to at least one of them.

摘要翻译： 本发明的N-乙酰基-D-半乳糖胺转移酶蛋白的特征在于将N-乙酰基-D-半乳糖胺转移至具有1,3-连接的N-乙酰基-D-葡糖胺，并且其优选具有所示的氨基酸序列 根据本发明的癌化测定使用在严格条件下与SEQ ID NO：1或3所示的核苷酸序列杂交的核酸或与至少一个氨基酸互补的核苷酸序列的核酸 的他们。

公开(公告)号：US20060177822A1

公开(公告)日：2006-08-10

申请号：US10539834

申请日：2003-12-26

申请人： Hisashi Narimatsu ,  Takashi Kudo ,  Akira Togayachi ,  Toru Hiruma

发明人： Hisashi Narimatsu ,  Takashi Kudo ,  Akira Togayachi ,  Toru Hiruma

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C07K14/82

CPC分类号： C12N9/1051

摘要： A tumor marker nucleic acid of the present invention is concerned with a nucleic acid hybridizing under stringent conditions to a nucleotide sequence described in SEQ ID NO: 1 or a complementary nucleotide sequence thereof. A method of testing canceration of the present invention is a method comprising diagnosing a biological sample as being cancerous when the transcription level of the nucleic acid in the biological sample significantly exceeds that in a normal biological sample as a control. The present invention also relates to a β1,3-N-acetyl-D-glucosaminyltransferase protein having an activity of transferring N-acetyl-D-glucosamine from a donor substrate to an acceptor substrate through β1,3-linkage.

公开(公告)号：US20150293104A1

公开(公告)日：2015-10-15

申请号：US14413846

申请日：2012-07-12

申请人： Hirofumi Nozaki ,  Takashi Ohkura ,  Atsushi Kuno ,  Maki Sogabe ,  Tomomi Kubota ,  Hiroyuki Kaji ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hayao Nakanishi ,  Toru Nakanishi

发明人： Hirofumi Nozaki ,  Takashi Ohkura ,  Atsushi Kuno ,  Maki Sogabe ,  Tomomi Kubota ,  Hiroyuki Kaji ,  Akira Togayachi ,  Yuzuru Ikehara ,  Hisashi Narimatsu ,  Hayao Nakanishi ,  Toru Nakanishi

IPC分类号： G01N33/574

CPC分类号： G01N33/57449 ,  C07K14/47 ,  G01N33/6842 ,  G01N2333/78 ,  G01N2333/8121 ,  G01N2333/902 ,  G01N2333/90287 ,  G01N2333/96458

摘要： An object of the present invention is to develop and provide an epithelial ovarian cancer diagnosis marker with which epithelial ovarian cancer can be detected inexpensively, conveniently, and low invasively with high accuracy, and a method for determining the presence or absence of epithelial ovarian cancer using the marker. The present invention provides a glycoprotein having a glycan-linked asparagine residue at a particular site of the glycoprotein secreted from an epithelial ovarian cancer cell, or a fragment thereof having the glycan as an epithelial ovarian cancer diagnosis marker. The present invention also provides a method for determining the presence or absence of epithelial ovarian cancer using the glycoprotein.

摘要翻译： 本发明的目的是开发和提供一种上皮性卵巢癌诊断标记，其可以廉价地，方便地和低侵入性高精度地检测上皮性卵巢癌，以及使用以下方法确定上皮性卵巢癌的存在或不存在的方法： 标记。 本发明提供一种在从上皮性卵巢癌细胞分泌的糖蛋白的特定部位或其具有聚糖作为上皮性卵巢癌诊断标记的片段的具有聚糖连接的天冬酰胺残基的糖蛋白。 本发明还提供了使用糖蛋白来确定上皮性卵巢癌的存在或不存在的方法。

公开(公告)号：US08431365B2

公开(公告)日：2013-04-30

申请号：US13539290

申请日：2012-06-29

申请人： Hisashi Narimatsu ,  Akira Togayachi ,  Niro Inaba ,  Toru Hiruma ,  Yasuko Ishizuka

发明人： Hisashi Narimatsu ,  Akira Togayachi ,  Niro Inaba ,  Toru Hiruma ,  Yasuko Ishizuka

IPC分类号： C12P19/26 ,  C12P19/18 ,  C12P21/06 ,  C12P19/34 ,  C12N9/10 ,  C07H21/04 ,  C07K1/00

CPC分类号： C12N9/1051

摘要： The N-acetyl-D-galactosamine transferase protein of the present invention is characterized by transferring N-acetyl-D-galactosamine to N-acetyl-D-glucosamine with β1,3 linkage, and it preferably has the amino acid sequence shown in SEQ ID NO: 2 or 4. The canceration assay according to the present invention uses a nucleic acid for measurement which hybridizes under stringent conditions to the nucleotide sequence shown in SEQ ID NO: 1 or 3 or a nucleotide sequence complementary to at least one of them.

摘要翻译： 本发明的N-乙酰基-D-半乳糖胺转移酶蛋白的特征在于将N-乙酰基-D-半乳糖胺转移至具有β1,3键的N-乙酰基-D-葡糖胺，并且其优选具有SEQ所示的氨基酸序列 根据本发明的癌化测定使用在严格条件下与SEQ ID NO：1或3所示的核苷酸序列杂交的核酸或与至少一种互补的核苷酸序列的核酸 。