公开(公告)号：US08901289B2

公开(公告)日：2014-12-02

申请号：US12775843

申请日：2010-05-07

申请人： Kyeong-Eun Jung ,  Alexei Kayushin ,  Moon Hee Kim ,  Kyung-Il Kim ,  Sungwon Kim ,  Yumi Ji

发明人： Kyeong-Eun Jung ,  Alexei Kayushin ,  Moon Hee Kim ,  Kyung-Il Kim ,  Sungwon Kim ,  Yumi Ji

IPC分类号： C07H21/02 ,  C07H19/167 ,  C07H19/067 ,  C07H19/10 ,  C07H19/20

CPC分类号： C07H21/02 ,  C07H1/00 ,  C07H19/067 ,  C07H19/10 ,  C07H19/167 ,  C07H19/20

摘要： The present invention provides a method for preparing nucleotide oligomers, including (a) coupling a nucleotide dimer or nucleotide trimer to a nucleoside attached to solid supports or to universal solid supports as a starting material; (b) sequentially coupling nucleotide monomers to the resulting structures of Step (a) to prepare a nucleotide oligomer; and (c) removing the nucleotide oligomers from the solid supports.The method of the present invention provides nucleotide oligomers having 15-20% higher purity than the conventional art. The present invention enables the efficient and inexpensive synthesis of nucleotide oligomers with high purity within a shorter period of time.

摘要翻译： 本发明提供了制备核苷酸寡聚体的方法，包括（a）将核苷酸二聚体或核苷酸三聚体与连接至固体支持物的核苷或作为起始材料的通用固体支持物偶联; （b）顺序地将核苷酸单体与步骤（a）的所得结构偶联以制备核苷酸寡聚物; 和（c）从固体支持物中除去核苷酸寡聚体。 本发明的方法提供了具有比常规技术高15-20％的核苷酸寡聚物。 本发明能够在更短的时间内高效且廉价地合成具有高纯度的核苷酸低聚物。

公开(公告)号：US20100273999A1

公开(公告)日：2010-10-28

申请号：US12775843

申请日：2010-05-07

申请人： Kyeong-Eun Jung ,  Alexei Kayushin ,  Moon Hee Kim ,  Kyung-Il Kim ,  Sungwon Kim ,  Yumi Ji

发明人： Kyeong-Eun Jung ,  Alexei Kayushin ,  Moon Hee Kim ,  Kyung-Il Kim ,  Sungwon Kim ,  Yumi Ji

IPC分类号： C07H1/00 ,  C07H21/00

CPC分类号： C07H21/02 ,  C07H1/00 ,  C07H19/067 ,  C07H19/10 ,  C07H19/167 ,  C07H19/20

摘要： The present invention provides a method for preparing nucleotide oligomers, including (a) coupling a nucleotide dimer or nucleotide trimer to a nucleoside attached to solid supports or to universal solid supports as a starting material; (b) sequentially coupling nucleotide monomers to the resulting structures of Step (a) to prepare a nucleotide oligomer; and (c) removing the nucleotide oligomers from the solid supports.The method of the present invention provides nucleotide oligomers having 15-20% higher purity than the conventional art. The present invention enables the efficient and inexpensive synthesis of nucleotide oligomers with high purity within a shorter period of time.

摘要翻译： 本发明提供了制备核苷酸寡聚体的方法，包括（a）将核苷酸二聚体或核苷酸三聚体与连接至固体支持物的核苷或作为起始材料的通用固体支持物偶联; （b）顺序地将核苷酸单体与步骤（a）的所得结构偶联以制备核苷酸寡聚物; 和（c）从固体支持物中除去核苷酸寡聚体。 本发明的方法提供了具有比常规技术高15-20％的核苷酸寡聚物。 本发明能够在更短的时间内高效且廉价地合成具有高纯度的核苷酸低聚物。