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    Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
    4.
    发明授权
    Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use 有权
    官能化3-炔基吡唑并嘧啶类似物作为通用碱基和使用方法

    公开(公告)号：US09085800B2

    公开(公告)日：2015-07-21

    申请号：US13429273

    申请日：2012-03-23

    申请人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

    发明人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6853 ,  C12Q1/6846 ,  C12Q2525/117

    摘要： 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

    摘要翻译： 提供3-炔基肌苷类似物及其作为通用碱基的用途。 肌苷类似物可以并入核酸引物和探针中。 它们不会使核酸双链体显着不稳定。 因此，结合肌苷类似物的新型核酸引物和探针可以以多种方法使用。 类似物作为普遍基础出人意料地起作用。 它们不仅能够稳定双链体，而且还要与A，C，T和G相反的次黄嘌呤稳定，但也可以通过聚合酶在引物中识别，从而进行有效的扩增。

    FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE
    5.
    发明申请
    FUNCTIONALIZED 3-ALKYNYL PYRAZOLOPYRIMIDINE ANALOGUES AS UNIVERSAL BASES AND METHODS OF USE 有权
    功能性3-烷基亚氨基吡嗪类似物作为通用基础和使用方法

    公开(公告)号：US20130261014A1

    公开(公告)日：2013-10-03

    申请号：US13827456

    申请日：2013-03-14

    申请人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

    发明人： Alexei Vorobiev ,  Eugeny A. Lukhtanov ,  Noah Scarr

    IPC分类号： C07H1/00

    CPC分类号： C07H1/00 ,  C12Q1/6816 ,  C12Q1/6846 ,  C12Q2525/117

    摘要： 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.

    摘要翻译： 提供3-炔基肌苷类似物及其作为通用碱基的用途。 肌苷类似物可以并入核酸引物和探针中。 它们不会使核酸双链体显着不稳定。 因此，结合肌苷类似物的新型核酸引物和探针可以以多种方法使用。 类似物作为普遍基础出人意料地起作用。 它们不仅能够稳定双链体，而且与A，C，T和G相反的次黄嘌呤稳定，而且在引物中也被聚合酶识别，从而进行有效的扩增。