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公开(公告)号：US08497115B2
公开(公告)日：2013-07-30
申请号：US13350384
申请日：2012-01-13
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US08852913B2
公开(公告)日：2014-10-07
申请号：US12639642
申请日：2009-12-16
申请人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
发明人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
CPC分类号： C12N9/2445 , A61K36/062 , C12P19/02 , C12P19/14 , C12Q1/6895 , C12Y302/01021
摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译： 本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。 本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。
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公开(公告)号：US07413888B2
公开(公告)日：2008-08-19
申请号：US10836063
申请日：2004-04-30
申请人： Ana Fidantsef , Michael Lamsa , Brian Gorre Clancy
发明人： Ana Fidantsef , Michael Lamsa , Brian Gorre Clancy
CPC分类号： C12N9/2445 , A61K36/062 , C12P19/02 , C12P19/14 , C12Q1/6895 , C12Y302/01021
摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译： 本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。 本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。
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公开(公告)号：US20080268526A1
公开(公告)日：2008-10-30
申请号：US12135611
申请日：2008-06-09
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
IPC分类号： C12P1/00
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US07393664B2
公开(公告)日：2008-07-01
申请号：US10837318
申请日：2004-04-30
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
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公开(公告)号：US20140011262A1
公开(公告)日：2014-01-09
申请号：US13945557
申请日：2013-07-18
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US07638616B2
公开(公告)日：2009-12-29
申请号：US12147333
申请日：2008-06-26
申请人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
发明人： Ana Fidantsef , Michael Lamsa , Brian Gorre-Clancy
CPC分类号： C12N9/2445 , A61K36/062 , C12P19/02 , C12P19/14 , C12Q1/6895 , C12Y302/01021
摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译： 本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。 本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。
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公开(公告)号：US20120122191A1
公开(公告)日：2012-05-17
申请号：US13350384
申请日：2012-01-13
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US20100099146A1
公开(公告)日：2010-04-22
申请号：US12639642
申请日：2009-12-16
申请人： Ana Fidantsef , Michael Lamsa , Brian Gorre Clancy
发明人： Ana Fidantsef , Michael Lamsa , Brian Gorre Clancy
CPC分类号： C12N9/2445 , A61K36/062 , C12P19/02 , C12P19/14 , C12Q1/6895 , C12Y302/01021
摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译： 本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。 本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。
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公开(公告)号：US20090053765A1
公开(公告)日：2009-02-26
申请号：US12147333
申请日：2008-06-26
申请人： Ana Fidantsef , Michael Lamsa , Brian Gorre Clancy
发明人： Ana Fidantsef , Michael Lamsa , Brian Gorre Clancy
CPC分类号： C12N9/2445 , A61K36/062 , C12P19/02 , C12P19/14 , C12Q1/6895 , C12Y302/01021
摘要： The present invention relates to variants of a parent beta-glucosidase, comprising a substitution at one or more positions corresponding to positions 142, 183, 266, and 703 of amino acids 1 to 842 of SEQ ID NO: 2 or corresponding to positions 142, 183, 266, and 705 of amino acids 1 to 844 of SEQ ID NO: 70, wherein the variant has beta-glucosidase activity. The present invention also relates to nucleotide sequences encoding the variant beta-glucosidases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
摘要翻译： 本发明涉及亲本β-葡糖苷酶的变体，其包含在对应于SEQ ID NO：2的氨基酸1至842的位置142,183,266和703的一个或多个位置处的取代或对应于位置142， SEQ ID NO：70的氨基酸1至844的183,266和705，其中所述变体具有β-葡糖苷酶活性。 本发明还涉及编码变体β-葡糖苷酶的核苷酸序列以及包含核苷酸序列的核酸构建体，载体和宿主细胞。
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