公开(公告)号：US09687190B2

公开(公告)日：2017-06-27

申请号：US13056469

申请日：2009-07-30

申请人： Anita Mahadevan-Jansen ,  Lisa White ,  John Phay ,  Constantine A. Paras ,  Elizabeth Kanter Bartz ,  Matthew D. Keller ,  Nicole Gasparino ,  Jennifer Gray Whisenant ,  Isaac J. Pence

发明人： Anita Mahadevan-Jansen ,  Lisa White ,  John Phay ,  Constantine A. Paras ,  Elizabeth Kanter Bartz ,  Matthew D. Keller ,  Nicole Gasparino ,  Jennifer Gray Whisenant ,  Isaac J. Pence

IPC分类号： A61B5/1455 ,  A61B5/00 ,  G01N21/64 ,  G01N21/65

CPC分类号： A61B5/415 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0086 ,  A61B5/418 ,  A61B5/4227 ,  A61B5/742 ,  A61B34/20 ,  A61B2034/2055 ,  A61B2505/05 ,  G01N21/6456 ,  G01N21/65 ,  G01N2021/6439 ,  G01N2201/0221

摘要： In one aspect, the present invention relates to a process for intra-operatively providing anatomical guidance in endocrine surgery. In one embodiment, the process includes the steps of illuminating tissues in the neck area of a living subject with a beam of light having a predetermined wavelength, obtaining Raman data from light scattered from the illuminated tissues, finding Raman signatures corresponding to thyroid or parathyroid tissues from the obtained Raman data, and identifying the thyroid or parathyroid tissues from the corresponding Raman signatures.

公开(公告)号：US20090069871A1

公开(公告)日：2009-03-12

申请号：US11945649

申请日：2007-11-27

申请人： Anita MAHADEVAN-JANSEN ,  Jonathon D. WELLS ,  E. Duco JANSEN

发明人： Anita MAHADEVAN-JANSEN ,  Jonathon D. WELLS ,  E. Duco JANSEN

IPC分类号： A61N5/067

CPC分类号： A61N5/0622 ,  A61N5/0618

摘要： The present invention, in one aspect, relates to a method for stimulating neural tissue of a living subject. In one embodiment, the method has the steps of generating at least one beam of radiation; introducing at least one of one or more chromophores and one or more optical agents to a target neural tissue; and delivering the at least one beam of radiation to the target neural tissue, wherein the at least one beam of radiation is delivered with a radiant exposure that causes a thermal gradient in the target neural tissue, thereby stimulating the target neural tissue.

摘要翻译： 本发明在一个方面涉及一种用于刺激活体的神经组织的方法。 在一个实施例中，该方法具有产生至少一个辐射束的步骤; 将一种或多种发色团和一种或多种光学剂中的至少一种引入靶神经组织; 以及将所述至少一束辐射递送到所述靶神经组织，其中所述至少一束辐射以辐射暴露递送，所述辐射暴露导致所述靶神经组织中的热梯度，从而刺激所述靶神经组织。

公开(公告)号：US20050119548A1

公开(公告)日：2005-06-02

申请号：US10955331

申请日：2004-09-30

申请人： Wei-Chiang Lin ,  Steven Toms ,  Anita Mahadevan-Jansen ,  Ravi Chari

发明人： Wei-Chiang Lin ,  Steven Toms ,  Anita Mahadevan-Jansen ,  Ravi Chari

IPC分类号： A61B20060101 ,  A61B5/00 ,  A61B6/00

CPC分类号： A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4244

摘要： A method for detecting death process of a cell or tissue of a living subject. In one embodiment, the method includes the steps of illuminating the cell or tissue of the living subject with a coherent light, collecting fluorescent light returned from the illuminated cell or tissue of the living subject, identifying a NAD(P)H peak of a spectrum of the collected fluorescent light with a wavelength, λpeak, and obtaining the intensity of the NAD(P)H peak of the spectrum of the collected fluorescent light substantially corresponding to the wavelength λpeak. These steps are repeated at sequential stages until the intensity of the NAD(P)H peak of the spectrum at a current stage is less than the intensity of the NAD(P)H peak of the spectrum at an earlier stage immediately prior to the current stage so as to detect death process of the cell of the living subject at the current stage using the intensity of the NAD(P)H peak of the spectrum.

摘要翻译： 一种用于检测活体受试者的细胞或组织的死亡过程的方法。 在一个实施例中，该方法包括以相干光照射活体的细胞或组织的步骤，收集从活体受试者的照射细胞或组织返回的荧光，识别光谱的NAD（P）H峰 收集的具有波长λλ峰值的荧光，并且获得基本上对应于波长λ的峰的峰的收集的荧光的光谱的NAD（P）H峰的强度， / SUB>。 这些步骤在连续阶段重复，直到当前阶段的光谱的NAD（P）H峰的强度小于在电流之前的较早阶段的光谱的NAD（P）H峰的强度 使用光谱的NAD（P）H峰的强度来检测当前阶段的活体的细胞的死亡过程。

公开(公告)号：US06377841B1

公开(公告)日：2002-04-23

申请号：US09545425

申请日：2000-04-07

申请人： Wei-Chiang Lin ,  Anita Mahadevan-Jansen ,  E. Duco Jansen ,  Steven A. Toms

发明人： Wei-Chiang Lin ,  Anita Mahadevan-Jansen ,  E. Duco Jansen ,  Steven A. Toms

IPC分类号： A61B600

CPC分类号： G01N21/6486 ,  A61B5/0059 ,  A61B5/4064 ,  G01N2021/6484

摘要： Optical spectroscopy for brain tumor demarcation was investigated in this study. Fluorescence and diffuse reflectance spectra were measured from normal and tumorous human brain tissues in vitro. A fluorescence peak was consistently observed around 460 nm (±10 nm) emission from both normal and tumorous brain tissues using 337 nm excitation. Intensity of this fluorescence peak (F460) from normal brain tissues was greater than that from primary brain tumorous tissues. In addition, diffuse reflectance (Rd) between 650 nm and 800 nm from white matter was significantly stronger than that from primary and secondary brain tumors. A good separation between gray matter and brain tumors was found using the ratio of F460 and Rd at 400 nm-600 nm. Two empirical discrimination algorithms based on F (400 nm-600 nm), Rd (600 nm-800 nm), and F (400 nm-600 nm)/Rd (400 nm-600 nm) were developed. These algorithms yielded an average sensitivity and specificity of 96% and 93%, respectively.

摘要翻译： 在本研究中研究了脑肿瘤分界的光学光谱。 从正常和肿瘤的人脑组织体外测量荧光和漫反射光谱。 使用337 nm激发，从正常和肿瘤的脑组织发现约460nm（±10nm）的荧光峰。 来自正常脑组织的这种荧光峰（F460）的强度大于原发性脑肿瘤组织的强度。 另外，白色物质在650 nm到800 nm之间的漫反射率（Rd）明显强于初级和次级脑肿瘤。 使用F460和Rd在400nm-600nm的比例，发现灰质和脑肿瘤之间的良好分离。 开发了基于F（400nm-600nm），Rd（600nm-800nm）和F（400nm-600nm）/ Rd（400nm-600nm）的两种经验辨别算法。 这些算法的平均灵敏度和特异度分别为96％和93％。

公开(公告)号：US5991653A

公开(公告)日：1999-11-23

申请号：US667993

申请日：1996-06-19

申请人： Rebecca Richards-Kortum ,  Anita Mahadevan-Jansen ,  Nirmala Ramanujam ,  Michele Follen Mitchell

发明人： Rebecca Richards-Kortum ,  Anita Mahadevan-Jansen ,  Nirmala Ramanujam ,  Michele Follen Mitchell

IPC分类号： A61B5/00 ,  G01N21/64 ,  G01N21/65 ,  G01N21/76 ,  G01N33/483 ,  A61B6/00

CPC分类号： G01N21/6486 ,  A61B5/0059 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4331 ,  G01N21/65 ,  A61B5/0086 ,  A61B5/7264 ,  G01N2021/656

摘要： Early diagnosis of cervical precancer is an important clinical goal. Optical spectroscopy has been suggested as a new technique to overcome limitations of current clinical practice. Herein, NIR Raman spectroscopy is applied to the diagnosis of cervical precancers. Using algorithms based on empirically selected peak intensities, ratios of peak intensities and a combination of Principal Component Analysis (PCA) for data reduction and Fisher Discriminant Analysis (FDA), normal tissues, inflammation and metaplasia were distinguishable from low grade and high grade precancers. The primary contributors to the tissue spectra appear to be collagen, nucleic acids, phospholipids and glucose 1-phosphate. These results suggest that near infrared Raman spectroscopy can be used effectively for cervical precancer diagnosis.

摘要翻译： 子宫颈癌的早期诊断是一个重要的临床目标。 光谱学被认为是克服当前临床实践的局限性的新技术。 在这里，NIR拉曼光谱法被应用于子宫颈预处理的诊断。 使用基于经验选择的峰值强度的算法，峰值强度的比率和用于数据降低的主成分分析（PCA）和Fisher判别分析（FDA），正常组织，炎症和化生的组合与低等级和高级别的预处理可以区分开。 组织光谱的主要贡献者似乎是胶原，核酸，磷脂和葡萄糖1-磷酸。 这些结果表明，近红外拉曼光谱可以有效地用于子宫颈癌前期诊断。

公开(公告)号：US20170046586A1

公开(公告)日：2017-02-16

申请号：US14822447

申请日：2015-08-10

申请人： Adnan Abbas ,  Melanie McWade ,  Anita Mahadevan-Jansen

发明人： Adnan Abbas ,  Melanie McWade ,  Anita Mahadevan-Jansen

IPC分类号： G06K9/32 ,  A61B5/00 ,  H04N9/31 ,  G06K9/20 ,  G06T7/00 ,  H04N5/33

CPC分类号： G06K9/3233 ,  A61B5/0071 ,  A61B5/0075 ,  A61B90/30 ,  A61B2090/366 ,  A61B2090/373 ,  A61B2505/05 ,  G06K9/2027 ,  G06K9/2054 ,  G06T7/0014 ,  G06T2207/30004 ,  H04N9/3185 ,  H04N9/3194

摘要： Devices and methods for producing a highlighted overlay of a region, including an illumination source configured to illuminate the region at a bandwidth containing a first wavelength at a working distance, an imager at a working distance configured to image the illuminated region at a bandwidth containing a second wavelength, where the first and second bandwidths and wavelengths are not visible, a visible light projector configured to illuminate the region and registered to the imager to produce alignment of imaged feature with projected features at the same location on the region, and a controller executing a program configured to filter acquired images from the imager to identify areas of the region of a predetermined light intensity, and control the projector to visibly highlight those areas on the region.

摘要翻译： 用于产生区域的突出覆盖的装置和方法，包括被配置为在工作距离处以包含第一波长的带宽照亮区域的照明源，工作距离处的成像器被配置成以包含 第二波长，其中第一和第二带宽和波长不可见，可见光投影器被配置为照亮该区域并被注册到成像器以产生成像特征与区域上相同位置处的投影特征的对准，以及控制器执行 程序，被配置为从所述成像器过滤所获取的图像，以识别所述区域的预定光强度的区域，并且控制所述投影仪以可视地突出显示所述区域上的那些区域。

公开(公告)号：US08498699B2

公开(公告)日：2013-07-30

申请号：US13013816

申请日：2011-01-26

申请人： Jonathon D. Wells ,  Mark P. Bendett ,  James S. Webb ,  Charles A. Lemaire ,  Austin R. Duke ,  E. Duco Jansen ,  Peter E. Konrad ,  Anita Mahadevan-Jansen

发明人： Jonathon D. Wells ,  Mark P. Bendett ,  James S. Webb ,  Charles A. Lemaire ,  Austin R. Duke ,  E. Duco Jansen ,  Peter E. Konrad ,  Anita Mahadevan-Jansen

IPC分类号： A61N1/00

CPC分类号： A61N1/36017 ,  A61B2018/2025 ,  A61N1/36082 ,  A61N1/378 ,  A61N5/0601 ,  A61N5/0613 ,  A61N5/0622 ,  A61N2005/063 ,  A61N2005/0659

摘要： An apparatus and method for stimulating animal tissue (for example to trigger a nerve action potential (NAP) signal in a human patient) by application of both electrical and optical signals for treatment and diagnosis purposes. The application of an electrical signal before or simultaneously to the application of a NAP-triggering optical signal allows the use of a lower amount of optical power or energy than would otherwise be needed if an optical signal alone was used for the same purpose and effectiveness. The application of the electrical signal may precondition the nerve tissue such that a lower-power optical signal can be used to trigger the desired NAP, which otherwise would take a higher-power optical signal were the electric signal not applied. Some embodiments include an implanted nerve interface having a plurality of closely spaced electrodes placed transversely and/or longitudinally to the nerve and a plurality of optical emitters.

摘要翻译： 一种用于刺激动物组织（例如在人类患者中触发神经动作电位（NAP）信号）的装置和方法）通过应用电信号和光信号用于治疗和诊断目的。 在应用NAP触发光信号之前或同时应用电信号允许使用比如果单独使用光信号用于相同目的和有效性时所需要的更少量的光功率或能量。 电信号的应用可以预处理神经组织，使得可以使用较低功率的光信号来触发所需的NAP，否则将在不施加电信号的情况下采取更高功率的光信号。 一些实施例包括植入的神经界面，其具有横向和/或纵向设置于神经的多个紧密间隔的电极以及多个光发射器。

公开(公告)号：US07979107B2

公开(公告)日：2011-07-12

申请号：US12537008

申请日：2009-08-06

申请人： Wei-Chiang Lin ,  Steven A. Toms ,  Anita Mahadevan-Jansen ,  Ravi S. Chari

发明人： Wei-Chiang Lin ,  Steven A. Toms ,  Anita Mahadevan-Jansen ,  Ravi S. Chari

IPC分类号： A61B5/05

CPC分类号： A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4244

摘要： A method for differentiating malignant in vivo liver tissues from normal in vivo liver tissues of a living subject includes the steps of: (a) illuminating a first area and a second area of in vivo liver tissues of the living subject with a first excitation light, (b) measuring an intensity of fluorescent light emitted from each of the first area and the second area of in vivo liver tissues in response to the first excitation light as a function of wavelength so as to obtain a first and a second fluorescent spectra, respectively, (c) illuminating the first area and the second area of in vivo liver tissues with a second excitation light, (d) measuring an intensity of diffuse light reflected by each of the first area and the second area of in vivo liver tissues in response to the second excitation light as a function of wavelength so as to obtain a first and a second diffused reflectance spectra, respectively, and (e) identifying one of the first area and the second area of in vivo liver tissues as malignant liver tissues and the other one of the first area and the second area of in vivo liver tissues as normal liver tissues, by comparing the first fluorescence spectrum and the second florescence spectrum, and comparing the first diffused reflection spectrum and the second diffused reflection spectrum.

摘要翻译： 用于区分恶性体内肝组织与活体受试者的正常体内肝组织的方法包括以下步骤：（a）用第一激发光照射活体的肝脏组织的第一区域和第二区域， （b）响应于作为波长的函数的第一激发光，测量从体内肝组织的第一区域和第二区域发射的荧光的强度，以分别获得第一和第二荧光光谱 （c）用第二激发光照射体内肝组织的第一区域和第二区域，（d）响应于测量由体内肝组织的第一区域和第二区域反映的漫射光的强度 作为波长的函数的第二激发光，以分别获得第一和第二扩散反射光谱，（e）识别体内肝脏的第一区域和第二区域之一 通过比较第一荧光光谱和第二荧光光谱，比较第一扩散反射光谱和第二扩散反应谱图，将组织作为恶性肝组织，将体内肝组织的第一区域和第二区域作为正常肝组织 反射光谱。

公开(公告)号：US20090292211A1

公开(公告)日：2009-11-26

申请号：US12537008

申请日：2009-08-06

申请人： Wei-Chiang LIN ,  Steven A. Toms ,  Anita Mahadevan-Jansen ,  Ravi S. Chari

发明人： Wei-Chiang LIN ,  Steven A. Toms ,  Anita Mahadevan-Jansen ,  Ravi S. Chari

IPC分类号： A61B6/00

CPC分类号： A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/4244

摘要： A method for detecting death process of a cell or tissue of a living subject. In one embodiment, the method includes the steps of illuminating the cell or tissue of the living subject with a coherent light, collecting fluorescent light returned from the illuminated cell or tissue of the living subject, identifying a NAD(P)H peak of a spectrum of the collected fluorescent light with a wavelength, λpeak, and obtaining the intensity of the NAD(P)H peak of the spectrum of the collected fluorescent light substantially corresponding to the wavelength λpeak. These steps are repeated at sequential stages until the intensity of the NAD(P)H peak of the spectrum at a current stage is less than the intensity of the NAD(P)H peak of the spectrum at an earlier stage immediately prior to the current stage so as to detect death process of the cell of the living subject at the current stage using the intensity of the NAD(P)H peak of the spectrum.

摘要翻译： 一种用于检测活体受试者的细胞或组织的死亡过程的方法。 在一个实施例中，该方法包括以相干光照射活体的细胞或组织的步骤，收集从活体受试者的照射细胞或组织返回的荧光，识别光谱的NAD（P）H峰 所收集的荧光的波长为lambdapeak，并且获得基本上对应于波长兰帕达克的所收集的荧光的光谱的NAD（P）H峰的强度。 这些步骤在连续阶段重复，直到当前阶段的光谱的NAD（P）H峰的强度小于在电流之前的较早阶段的光谱的NAD（P）H峰的强度 使用光谱的NAD（P）H峰的强度来检测当前阶段的活体的细胞的死亡过程。

公开(公告)号：US20090021724A1

公开(公告)日：2009-01-22

申请号：US11780793

申请日：2007-07-20

申请人： Anita MAHADEVAN-JANSEN ,  Ton Van Leeuwen ,  Chetan A. Patil

发明人： Anita MAHADEVAN-JANSEN ,  Ton Van Leeuwen ,  Chetan A. Patil

IPC分类号： G01N21/00

CPC分类号： G01N21/4795 ,  A61B5/0066 ,  A61B5/0075 ,  A61B5/444 ,  A61B5/445 ,  G01B9/0203 ,  G01B9/02089 ,  G01B9/02091 ,  G01B2290/35 ,  G01N21/65

摘要： An apparatus for evaluating a target of interest of a living subject. In one embodiment, the apparatus has a first light source for generating a broadband light, a second light source for generating a monochromatic light, a beamsplitter optically coupled to the first light source for receiving the broadband light and splitting it into a reference light and a sample light, a reference arm optically coupled to the beamsplitter for receiving the reference light and returning it into the beamsplitter, and a probe having a working end placed proximal to a target of interest of a living subject, optically coupled to the beamsplitter and the second light source for receiving the sample light and the monochromatic light, delivering them from the working end to the target of interest, collecting from the working end a backscattering light and a Raman scattering light that are obtained from interaction of the sample light and the monochromatic light with the target of interest, respectively, and returning the backscattering light into the beamsplitter so as to generate an interference signal between the returned backscattering light and the returned reference light in the beamsplitter.

摘要翻译： 一种用于评估活体感兴趣的目标的装置。 在一个实施例中，该装置具有用于产生宽带光的第一光源，用于产生单色光的第二光源，光学耦合到第一光源的分束器，用于接收宽带光并将其分割为参考光和 样品光，参考臂，其光学耦合到分束器，用于接收参考光并将其返回到分束器中;以及探针，其具有放置在活体的感兴趣目标附近的工作端，光学耦合到分束器，第二 用于接收样品光和单色光的光源，将其从工作端传送到感兴趣的目标，从工作端收集从样品光和单色光的相互作用获得的后向散射光和拉曼散射光 分别与感兴趣的目标，并将后向散射光返回到分束器中以便发射 消除返回的后向散射光和分束器中返回的参考光之间的干扰信号。