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    Method of selecting hla-dp4 ligands and the applications thereof
    2.
    发明申请
    Method of selecting hla-dp4 ligands and the applications thereof 失效
    选择hla-dp4配体的方法及其应用

    公开(公告)号：US20050059107A1

    公开(公告)日：2005-03-17

    申请号：US10491891

    申请日：2002-10-17

    申请人： Bernard Maillere ,  Florence Castelli ,  Cecile Buhot ,  Bertrand Georges

    发明人： Bernard Maillere ,  Florence Castelli ,  Cecile Buhot ,  Bertrand Georges

    IPC分类号： A61P37/02 ,  C07K14/47 ,  C12N15/12 ,  G01N33/68 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543

    CPC分类号： G01N33/5008 ,  C07K14/47 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5091 ,  G01N33/68

    摘要： The invention relates to a method of selecting HLA-DP4 ligand molecules comprising the following steps: (i) incubation of purified HLA-DP4 with a tracer having general formula (I) Z1X1X2X3X4X5X6X7X8X9Z2 (wherein Z1 and Z2, which may be identical or different, are equal to zero or each represent a peptide with between 1 and 100 amino acids; X6 represents an aromatic or hydrophobic amino acid or a cysteine; and X1 represents an aromatic or hydrophobic amino acid and/or X9 represents an aromatic or hydrophobic amino acid or C, D, Q, S, T or E; and X2,X3,X4,X5,X7 and X8 each represent an amino acid) in the presence of different concentrations of molecule(s) to be tested; (ii) separation of the different complexes formed; (iii) detection of HLA-DP4/peptide tracer complexes; and (iv) selection of ligand molecules having a binding capacity IC50

    摘要翻译： 本发明涉及一种选择HLA-DP4配体分子的方法，包括以下步骤：（i）将纯化的HLA-DP4与具有通式（I）Z1X1X2X3X4X5X6X7X8X9Z2的示踪剂（其中Z1和Z2可以相同或不同） 等于零或每个表示具有1至100个氨基酸的肽; X 6表示芳族或疏水性氨基酸或半胱氨酸;且X 1表示芳族或疏水性氨基酸和/或X 9表示芳族或疏水性氨基酸或 C，D，Q，S，T或E;而X2，X3，X4，X5，X7和X8各自表示氨基酸） （ii）形成的不同络合物的分离; （iii）检测HLA-DP4 /肽示踪剂复合物; 和（iv）选择具有结合能力IC50 <1000nM的配体分子。 本发明还涉及其应用。

    METHOD OF SELECTING HLA-DP4 LIGANDS AND THE APPLICATIONS THEREOF
    4.
    发明申请
    METHOD OF SELECTING HLA-DP4 LIGANDS AND THE APPLICATIONS THEREOF 审中-公开
    选择HLA-DP4配体的方法及其应用

    公开(公告)号：US20100324829A1

    公开(公告)日：2010-12-23

    申请号：US12719712

    申请日：2010-03-08

    申请人： Bernard MAILLERE ,  Florence Castelli ,  Cécile Buhot ,  Bertrand Georges

    发明人： Bernard MAILLERE ,  Florence Castelli ,  Cécile Buhot ,  Bertrand Georges

    IPC分类号： G06F19/00

    CPC分类号： G01N33/5008 ,  C07K14/47 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/5091 ,  G01N33/68

    摘要： A method of selecting a test molecule that binds to HLA-DP4 by (i) incubating HLA-DP4 with the test molecule and a labeled peptide of formula (I) Z1X1X2X3X4X5X6X7X8X9Z2 thereby forming respective complexes, wherein Z1 and Z2, which may be identical or different, are each either zero or from 1 to 100 amino acids; X1 is an aromatic or hydrophobic amino acid, or S; X6 is an aromatic or hydrophobic amino acid, or C; X9 is an aromatic or hydrophobic amino acid, or C, D, Q, S, T, or E; and X2, X3, X4, X5, X7 and X8 are each an amino acid; (ii) separating the respective complexes formed; (iii) detecting the HLA-DP4/labeled peptide complexes; and (iv) selecting the test molecule that exhibits a binding activity of IC50

    摘要翻译： 通过（i）将HLA-DP4与测试分子和式（I）Z1X1X2X3X4X5X6X7X8X9Z2的标记肽一起孵育从而形成各自的复合物，选择与HLA-DP4结合的测试分子的方法，其中Z1和Z2可以相同或相同， 不同的，各自为零或1至100个氨基酸; X1是芳香族或疏水性氨基酸，或S; X6是芳香族或疏水性氨基酸，或C; X9是芳族或疏水性氨基酸，或C，D，Q，S，T或E; X2，X3，X4，X5，X7和X8各自为氨基酸; （ii）分离形成的各个复合物; （iii）检测HLA-DP4 /标记的肽复合物; 和（iv）选择显示IC50 <1000nM的结合活性的测试分子，其对应于抑制标记肽的竞争性HLA-DP4结合的50％的测试分子的浓度。