公开(公告)号：US20080170224A1

公开(公告)日：2008-07-17

申请号：US11724818

申请日：2007-03-16

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

IPC: G01J3/30

CPC classification number: G01N21/6428 ,  G01N2021/6441

Abstract: Apparatus and method for the stimulation and collection of fluorescent signals, said apparatus comprising: i) a frequency modulated optical source which emits excitation light of a wavelength which will stimulate a target fluorophore to emit a fluorescent signal when illuminated by said excitation light: ii) a first optical filter interposed between said optical source and said target fluorophore, iii) a second optical filter interposed between said fluorophore and a photo-detector positioned to receive said emitted fluorescent signal, and iv) a data processor which calculates and records the phase delay between said excitation light and said fluorescent signal wherein the beam path of at least one of the excitation light and the fluorescent signal is transmitted substantially through free space.

Abstract translation: 用于刺激和收集荧光信号的装置和方法，所述装置包括：i）调频光源，其发射波长的激发光，其将在被所述激发光照射时刺激目标荧光团发射荧光信号：ii） 插入在所述光源和所述目标荧光团之间的第一滤光器，iii）置于所述荧光团和位于接收所述发射的荧光信号的光检测器之间的第二滤光器，以及iv）数据处理器，其计算并记录相位延迟 在所述激发光和所述荧光信号之间，其中激发光和荧光信号中的至少一个的光束路径基本上透过自由空间传播。

公开(公告)号：US07968840B2

公开(公告)日：2011-06-28

申请号：US12290206

申请日：2008-10-28

Applicant: Benjamin Blizard ,  Charles Kamas ,  Edward Pruitt

Inventor： Benjamin Blizard ,  Charles Kamas ,  Edward Pruitt

IPC: G01D18/00

CPC classification number: G01N21/6428

Abstract: A validation apparatus for testing the measurement accuracy of a phase fluorimeter comprising: i) a photodetector responsive to the excitation light emitted by a phase fluorimeter; ii) electronics connected to the detector which calculates the phase of the light emitted by the phase fluorimeter and causes a light source in the validation apparatus to emit light of a wave length which will stimulate the detector of the phase fluorimeter but which is phase shifted relative to the light emitted by the phase fluorimeter.

Abstract translation: 一种用于测试相位荧光计的测量精度的验证装置，包括：i）响应于由相位荧光计发射的激发光的光电检测器; ii）连接到检测器的电子计算机，其计算由相位荧光计发射的光的相位，并且使验证装置中的光源发射波长的光，该波长将刺激相位荧光计的检测器，但是相移相对 对于由相位荧光计发出的光。

公开(公告)号：US20080171383A1

公开(公告)日：2008-07-17

申请号：US11725255

申请日：2007-03-17

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

IPC: C12M1/34

CPC classification number: C12M23/14 ,  C12M29/00 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34

Abstract: A port assembly for use with a polymeric bioreactor bag said assembly comprising: i) a hollow port member comprised at least in part of a material suitable to be fusibly affixed to the wall surface of said bioreactor bag; ii) at least one fluorophore spot positioned on said port member; iii) conduit means for conveying excitation light from an optical source to said fluorophore, said conduit means being an assembly comprising at least one of a lens, an optic fiber, a curved parabolic collimator, a shaped reflector or a wave guide; iv) conduit means for conveying fluorescent emission light from said fluorophore to a photo-detector, said conduit means being an assembly comprising at least one of a lens, an optic fiber, a curved parabolic collimator, a shaped reflector or a wave guide.

Abstract translation: 一种与聚合物生物反应器袋一起使用的端口组件，所述组件包括：i）中空端口构件，其至少部分地包括适于被柔性地固定到所述生物反应器袋的壁表面的材料; ii）位于所述端口构件上的至少一个荧光团斑点; iii）用于将来自光源的激发光传送到所述荧光团的导管装置，所述导管装置是包括透镜，光纤，弯曲抛物线准直器，成形反射器或波导中的至少一个的组件; iv）用于将来自所述荧光团的荧光发射光传送到光检测器的导管装置，所述导管装置是包括透镜，光纤，弯曲抛物线准直仪，成形反射器或波导中的至少一个的组件。

公开(公告)号：US07824902B2

公开(公告)日：2010-11-02

申请号：US11725255

申请日：2007-03-17

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

IPC: C12M1/34

CPC classification number: C12M23/14 ,  C12M29/00 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34

Abstract: A port assembly for use with a polymeric bioreactor bag includes the following components: i) a hollow port member made from a material which can be fusibly affixed to the wall surface of the bioreactor bag; ii) at least one fluorophore spot positioned on the port member; iii) a conduit for conveying excitation light from an optical source to the fluorophore, which conduit can be a lens, a curved parabolic collimator, a shaped reflector or a wave guide; and iv) a second conduit for conveying fluorescent emission light from the excited fluorophore to a photo-detector, which second conduit although different from the first, can likewise be a lens, a curved parabolic collimator, a shaped reflector or a wave guide.

Abstract translation: 与聚合物生物反应器袋一起使用的端口组件包括以下部件：i）由材料制成的中空端口部件，该材料可以柔软地固定在生物反应器袋的壁表面上; ii）位于端口构件上的至少一个荧光团斑点; iii）用于将激发光从光源传输到荧光团的导管，该导管可以是透镜，弯曲抛物线准直器，成形反射器或波导管; 以及iv）用于将来自激发的荧光团的荧光发射光传送到光检测器的第二导管，该第二导管虽然不同于第一导管，但也可以是透镜，弯曲的抛物线准直器，成形反射器或波导。

公开(公告)号：US20100102214A1

公开(公告)日：2010-04-29

申请号：US12290206

申请日：2008-10-28

Applicant: Benjamin Blizard ,  Edward Pruitt ,  Charles Kamas

Inventor： Benjamin Blizard ,  Edward Pruitt ,  Charles Kamas

IPC: G01N37/00

CPC classification number: G01N21/6428

Abstract: A validation apparatus for testing the measurement accuracy of a phase fluorimeter comprising:i) a photodetector responsive to the excitation light emitted by a phase fluorimeter; ii) electronics connected to the detector which calculates the phase of the light emitted by the phase fluorimeter and causes a light source in the validation apparatus to emit light of a wave length which will stimulate the detector of the phase fluorimeter but which is phase shifted relative to the light emitted by the phase fluorimeter.

Abstract translation: 一种用于测试相位荧光计的测量精度的验证装置，包括：i）响应于由相位荧光计发射的激发光的光电检测器; ii）连接到检测器的电子计算机，其计算由相位荧光计发射的光的相位，并且使验证装置中的光源发射波长的光，该波长将刺激相位荧光计的检测器，但是相移相对 对于由相位荧光计发出的光。

公开(公告)号：US07489402B2

公开(公告)日：2009-02-10

申请号：US11724818

申请日：2007-03-16

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N2021/6441

Abstract: An apparatus and method for the stimulation and collection of fluorescent signals from a target analyte. The apparatus comprises: i) a frequency modulated optical source which emits excitation light of a wavelength which will stimulate a target fluorophore to emit a fluorescent signal when illuminated by the excitation light, ii) a first optical filter interposed between the optical source and the target fluorophore, iii) a second optical filter interposed between the fluorophore and a photo-detector and positioned to receive the emitted fluorescent signal. The beam path of at least one, preferably both, of the excitation light and the fluorescent signal is transmitted substantially through free space. A data processor will normally be used to calculate and record the phase delay between the excitation light and the fluorescent signal.

Abstract translation: 用于刺激和收集来自目标分析物的荧光信号的装置和方法。 该装置包括：i）调频光源，其发射波长的激发光，该激发光将在被激发光照射时刺激目标荧光团发出荧光信号，ii）插入在光源和靶之间的第一光学滤波器 荧光团，iii）插入在所述荧光团和光检测器之间并定位成接收所发射的荧光信号的第二光学滤光器。 激发光和荧光信号的至少一个，优选两个的光束路径基本上透过自由空间传播。 通常将使用数据处理器来计算和记录激发光和荧光信号之间的相位延迟。