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公开(公告)号:US20120276209A1
公开(公告)日:2012-11-01
申请号:US13464690
申请日:2012-05-04
申请人: Pieter R. Cullis , Nathan M. Belliveau , Carl Lars Genghis Hansen , Jens Huft , James Taylor , Andre Wild , Stuart Malcolm , Ismail Hafez , Alex Leung , David Walker
发明人: Pieter R. Cullis , Nathan M. Belliveau , Carl Lars Genghis Hansen , Jens Huft , James Taylor , Andre Wild , Stuart Malcolm , Ismail Hafez , Alex Leung , David Walker
IPC分类号: A61K31/7088 , A61K9/50 , C12N5/071
CPC分类号: C12N15/88 , A61K9/1272 , A61K9/145 , A61K9/146 , A61K31/7088 , A61K47/22 , C12N15/111 , C12N15/113 , C12N2310/14 , C12N2320/32
摘要: Lipid particles containing a nucleic acid, devices and methods for making the lipid particles, and methods for using the lipid particles.
摘要翻译: 含有核酸的脂质颗粒,用于制备脂质颗粒的装置和方法,以及使用脂质颗粒的方法。
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公开(公告)号:US09902990B2
公开(公告)日:2018-02-27
申请号:US14122653
申请日:2011-05-27
申请人: Carl Lars Genghis Hansen , Michael VanInsberghe , Adam White , Oleh Petriv , Tim Leaver , Anupam Singhal , William Bowden , Veronique Lecault , Dan Da Costa , Leo Wu , Georgia Russell , Darek Sikorski
发明人: Carl Lars Genghis Hansen , Michael VanInsberghe , Adam White , Oleh Petriv , Tim Leaver , Anupam Singhal , William Bowden , Veronique Lecault , Dan Da Costa , Leo Wu , Georgia Russell , Darek Sikorski
IPC分类号: C12Q1/68 , G01N33/50 , G01N33/543 , G01N33/569 , C12M3/06 , B01L3/00 , G01N1/34 , B01L7/00
CPC分类号: C12Q1/6806 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0668 , B01L2300/0816 , B01L2400/086 , C12M23/16 , C12Q1/6844 , G01N1/34 , G01N33/5005 , G01N33/54366 , G01N33/569
摘要: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.
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公开(公告)号:US09758795B2
公开(公告)日:2017-09-12
申请号:US13464690
申请日:2012-05-04
申请人: Pieter R. Cullis , Nathan M. Belliveau , Carl Lars Genghis Hansen , Jens Huft , James Taylor , Andre Wild , Stuart Malcolm , Ismail Hafez , Alex Leung , David Walker
发明人: Pieter R. Cullis , Nathan M. Belliveau , Carl Lars Genghis Hansen , Jens Huft , James Taylor , Andre Wild , Stuart Malcolm , Ismail Hafez , Alex Leung , David Walker
IPC分类号: C12N15/88 , A61K47/00 , A61K47/48 , A61K9/14 , A61K9/127 , A61K31/7088 , A61K47/22 , C12N15/11
CPC分类号: C12N15/88 , A61K9/1272 , A61K9/145 , A61K9/146 , A61K31/7088 , A61K47/22 , C12N15/111 , C12N15/113 , C12N2310/14 , C12N2320/32
摘要: Lipid particles containing a nucleic acid, devices and methods for making the lipid particles, and methods for using the lipid particles.
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公开(公告)号:US09211537B2
公开(公告)日:2015-12-15
申请号:US12741880
申请日:2008-11-07
CPC分类号: B01L3/5027 , B01J2219/00317 , B01J2219/00396 , B01J2219/00398 , B01J2219/00495 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , B01L3/5025 , B01L3/502723 , B01L3/50273 , B01L3/50851 , B01L7/52 , B01L2200/0605 , B01L2200/0621 , B01L2200/0642 , B01L2200/0689 , B01L2200/16 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , G06F3/017 , G06F3/04815 , G06F3/04842
摘要: A microfluidic device comprising a plurality of reaction chambers in fluid communication with a flow channel formed in an elastomeric substrate, a vapor barrier for preventing evaporation from the plurality of reaction chambers, and a continuous phase fluid for isolation of each of the plurality of reaction chambers.
摘要翻译: 一种微流体装置,包括与形成在弹性体基底中的流动通道流体连通的多个反应室,用于防止来自多个反应室的蒸发的蒸汽屏障,以及用于隔离多个反应室中的每一个的连续相流体 。
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公开(公告)号:US20110053151A1
公开(公告)日:2011-03-03
申请号:US12741880
申请日:2008-11-07
CPC分类号: B01L3/5027 , B01J2219/00317 , B01J2219/00396 , B01J2219/00398 , B01J2219/00495 , B01J2219/00529 , B01J2219/00596 , B01J2219/00608 , B01J2219/00659 , B01J2219/00722 , B01L3/5025 , B01L3/502723 , B01L3/50273 , B01L3/50851 , B01L7/52 , B01L2200/0605 , B01L2200/0621 , B01L2200/0642 , B01L2200/0689 , B01L2200/16 , B01L2300/0819 , B01L2300/0829 , B01L2300/0864 , B01L2300/123 , B01L2300/1805 , B01L2400/0487 , G06F3/017 , G06F3/04815 , G06F3/04842
摘要: A microfluidic device comprising a plurality of reaction chambers in fluid communication with a flow channel formed in an elastomeric substrate, a vapor barrier for preventing evaporation from the plurality of reaction chambers, and a continuous phase fluid for isolation of each of the plurality of reaction chambers.
摘要翻译: 一种微流体装置,包括与形成在弹性体基底中的流动通道流体连通的多个反应室,用于防止来自多个反应室的蒸发的蒸汽屏障,以及用于隔离多个反应室中的每一个的连续相流体 。
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