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31 条记录 (耗时 0.067 秒)

    Expression and secretion vector in yeasts, useful for preparing heterologous proteins
    4.
    发明授权
    Expression and secretion vector in yeasts, useful for preparing heterologous proteins 失效
    酵母中的表达和分泌载体，用于制备异源蛋白质

    公开(公告)号：US5432082A

    公开(公告)日：1995-07-11

    申请号：US069455

    申请日：1993-06-01

    申请人： Cesira Galeotti ,  Emanuela Palla ,  Giovanni Raugei ,  Giuliano Bensi ,  Maria L. Melli

    发明人： Cesira Galeotti ,  Emanuela Palla ,  Giovanni Raugei ,  Giuliano Bensi ,  Maria L. Melli

    IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/39 ,  C07K14/54 ,  C07K14/545 ,  C12N1/16 ,  C12N15/81 ,  C12P21/00 ,  C12R1/865 ,  C12N15/70 ,  C12N15/25

    CPC分类号： C12N15/815 ,  C07K14/39 ,  C07K14/54 ,  C07K14/545 ,  C12N15/81 ,  A61K38/00 ,  C07K2319/02

    摘要： The invention provides an expression and secretion vector in yeasts which is useful for preparing heterologous proteins, comprising a synthetic oligonucleotide which directs the secretion of the heterologous protein wherein the synthetic oligonucleotide is positioned between the inducible hybrid promoter GAL-CYC and a multiple-site polylinker followed by the signals of transcription termination recognized by the RNA polymerase of the yeasts. The invention includes a hybrid plasmid obtained by cloning in one of the restriction sites of the polylinker of the vector, the DNA sequence which codes for a heterologous protein. A process is also described for the preparation of heterologous proteins which comprises cultivating in a suitable culture medium a yeast transformed with the hybrid plasmid and recovering from the culture medium the resulting heterologous proteins. The recovered proteins include hormones, lymphokines, viral antigens or immunogens useful in the therapeutical and diagnostic field.

    摘要翻译： 本发明提供在酵母中的表达和分泌载体，其可用于制备异源蛋白质，其包含指导异源蛋白质分泌的合成寡核苷酸，其中合成的寡核苷酸位于诱导型杂合启动子GAL-CYC和多位点多接头 其后是由酵母的RNA聚合酶识别的转录终止信号。 本发明包括通过克隆载体的多接头的限制性位点之一获得的杂交质粒，其编码异源蛋白质的DNA序列。 还描述了用于制备异源蛋白质的方法，其包括在合适的培养基中培养用杂交质粒转化的酵母，并从培养基中回收得到的异源蛋白质。 回收的蛋白质包括可用于治疗和诊断领域的激素，淋巴因子，病毒抗原或免疫原。

    Neisserial antigenic peptides
    6.
    发明授权
    Neisserial antigenic peptides 有权
    Neisserial抗原肽

    公开(公告)号：US09067987B2

    公开(公告)日：2015-06-30

    申请号：US13405213

    申请日：2012-02-24

    申请人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Marirosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

    发明人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Marirosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

    IPC分类号： C07K14/22 ,  A61K39/095 ,  A61K39/00 ,  A61K48/00

    CPC分类号： A61K39/095 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/55505 ,  C07K14/22

    摘要： This invention provides, among other things, proteins, polypeptides, and fragments thereof, derived from the bacteria Neisseria meningitidis B. Also provided are nucleic acids encoding for such proteins, polypeptides, and/or fragments, as well as nucleic acids complementary thereto e.g., antisense nucleic acids). Additionally, this invention provides antibodies which bind to the proteins, polypeptides, and/or fragments. This invention further provides expression vectors useful for making the proteins, polypeptides, and/or fragments, as well as host cells transformed with such vectors. This invention also provides compositions of the proteins, polypeptides, fragments, and/or nucleic acids, for use as vaccines, diagnostic reagents, immunogenic compositions, and the like. Methods of making the compositions and methods of treatment with the compositions are also provided. This invention also provides methods of detecting the proteins, polypeptides, fragments, and/or nucleic acids.

    摘要翻译： 本发明尤其提供衍生自脑膜炎奈瑟氏球菌B细菌的蛋白质，多肽和其片段。还提供编码这种蛋白质，多肽和/或片段的核酸，以及与其互补的核酸，例如， 反义核酸）。 另外，本发明提供了结合蛋白质，多肽和/或片段的抗体。 本发明进一步提供了可用于制备蛋白质，多肽和/或片段以及用这些载体转化的宿主细胞的表达载体。 本发明还提供用作疫苗，诊断试剂，免疫原性组合物等的蛋白质，多肽，片段和/或核酸的组合物。 还提供了用组合物制备组合物和处理方法的方法。 本发明还提供了检测蛋白质，多肽，片段和/或核酸的方法。

    NEISSERIAL ANTIGENIC PEPTIDES
    7.
    发明申请
    NEISSERIAL ANTIGENIC PEPTIDES 有权
    NEISSERIAL抗原肽

    公开(公告)号：US20120276129A1

    公开(公告)日：2012-11-01

    申请号：US13405213

    申请日：2012-02-24

    申请人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Mariarosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

    发明人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Mariarosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

    IPC分类号： A61K39/095 ,  A61P37/04 ,  C07K14/22

    CPC分类号： A61K39/095 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/55505 ,  C07K14/22

    摘要： This invention provides, among other things, proteins, polypeptides, and fragments thereof, derived from the bacteria Neisseria meningitidis B. Also provided are nucleic acids encoding for such proteins, polypeptides, and/or fragments, as well as nucleic acids complementary thereto e.g., antisense nucleic acids). Additionally, this invention provides antibodies which bind to the proteins, polypeptides, and/or fragments. This invention further provides expression vectors useful for making the proteins, polypeptides, and/or fragments, as well as host cells transformed with such vectors. This invention also provides compositions of the proteins, polypeptides, fragments, and/or nucleic acids, for use as vaccines, diagnostic reagents, immunogenic compositions, and the like. Methods of making the compositions and methods of treatment with the compositions are also provided. This invention also provides methods of detecting the proteins, polypeptides, fragments, and/or nucleic acids.

    摘要翻译： 本发明尤其提供衍生自脑膜炎奈瑟氏球菌B细菌的蛋白质，多肽和其片段。还提供编码这种蛋白质，多肽和/或片段的核酸，以及与其互补的核酸，例如， 反义核酸）。 另外，本发明提供了结合蛋白质，多肽和/或片段的抗体。 本发明进一步提供了可用于制备蛋白质，多肽和/或片段以及用这些载体转化的宿主细胞的表达载体。 本发明还提供用作疫苗，诊断试剂，免疫原性组合物等的蛋白质，多肽，片段和/或核酸的组合物。 还提供了用组合物制备组合物和处理方法的方法。 本发明还提供了检测蛋白质，多肽，片段和/或核酸的方法。