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公开(公告)号：US07674588B2
公开(公告)日：2010-03-09
申请号：US12069724
申请日：2008-02-11
CPC分类号： G01N33/542 , G01N2500/00
摘要： Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to a second fluorophore, which forms a FRET pair with the first fluorophore. The bound chemical entities are exposed to radiation at an excitation frequency for either the first or the second fluorophore, and polarization anisotropy of a FRET fluorescent signal from the bound chemical entities is measured to detect specific binding between the first and second chemical entities. Techniques are also disclosed for detecting whether a FRET interaction is occurring between a first chemical entity including a donor fluorophore and a second chemical entity including an acceptor fluorophore, using simultaneous anisotropy measurements at the wavelengths of the donor and acceptor fluorophores.
摘要翻译： 描述了用于检测第一和第二化学实体之间的特异性结合的方法和装置。 与第一荧光团相关联的第一化学实体被固定化。 允许第二化学实体与固定化的第一化学实体结合。 第二化学实体是或连接到与第一荧光团形成FRET对的第二荧光团。 结合的化学实体以第一或第二荧光团的激发频率暴露于辐射，并且测量来自结合的化学实体的FRET荧光信号的偏振各向异性以检测第一和第二化学实体之间的特异性结合。 还公开了用于检测在包括供体荧光团的第一化学实体和包括受体荧光团的第二化学实体之间是否发生FRET相互作用的技术，其使用在供体和受体荧光团的波长处的同时各向异性测量。
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公开(公告)号：US06825042B1
公开(公告)日：2004-11-30
申请号：US09723759
申请日：2000-11-27
IPC分类号： G01N3502
CPC分类号： B01L3/5085 , B01J2219/00315 , B01J2219/00317 , B01J2219/00659 , B01J2219/00707 , B01J2219/0072 , B01L9/52 , B01L9/523 , B01L2300/0829 , C40B60/14 , Y10T436/11 , Y10T436/113332 , Y10T436/114165
摘要： A microplate lid having a substantially flat, generally rectangular top with first and second pairs of opposite sides. A flange depends from the top of the first and second pairs of sides, the flange bearings at least one recess area along each side of the first pair of sides, the recessed area extended into the top.
摘要翻译： 一种微板盖，其具有基本平坦的，大致矩形的顶部，其具有第一和第二对相对侧。 凸缘从第一对侧面和第二对侧面的顶部开始，凸缘轴承沿着第一对侧面的每一侧的至少一个凹部区域，凹陷区域延伸到顶部。
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公开(公告)号：US5324480A
公开(公告)日：1994-06-28
申请号：US986423
申请日：1992-12-04
CPC分类号： G01N35/1002 , G01N1/405 , G01N2035/103 , G01N30/24 , G01N35/028 , Y10T137/87571
摘要： A liquid handling system includes a translation mechanism, and a probe mounted on the translation mechanism. The probe has a number of lumens extending along the length of the probe. The lumens are open at their lower ends and connectable at their upper ends to a compressed air source and sources of liquid solvents. The system further includes multiple seal heads that can be attached to the lower end of the probe to perform various functions. Some seal heads seal to the tops of various sized containers placed below the probe, with a cylindrical splash guard overlying the exterior of the top of the container to prevent splashes that might injure personnel. Other seal heads rotatably attach to other structures, such as filters. The seal heads may be parked and then used, one at a time, by inserting the probe into the appropriate seal head. After use, the seal head is returned to the parking location and drawn off the end of the probe.
摘要翻译： 液体处理系统包括平移机构和安装在平移机构上的探头。 探头具有沿探头长度延伸的多个腔。 管腔在其下端开放，并在其上端连接到压缩空气源和液体溶剂源。 该系统还包括可以附接到探针的下端以执行各种功能的多个密封头。 一些密封头密封到放置在探头下方的各种尺寸的容器的顶部，一个圆柱形防溅罩覆盖在容器顶部的外部，以防止可能伤害人员的飞溅。 其他密封头可旋转地附接到其他结构，例如过滤器。 密封头可以通过将探头插入适当的密封头中而一次停放并随后使用。 使用后，密封头返回到停车位置，并从探头的末端拉出。
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公开(公告)号：US20080206888A1
公开(公告)日：2008-08-28
申请号：US12069724
申请日：2008-02-11
IPC分类号： G01N33/566 , G01N21/76
CPC分类号： G01N33/542 , G01N2500/00
摘要： Methods and apparatus are described for detecting specific binding between first and second chemical entities. The first chemical entity in association with a first fluorophore is immobilized. The second chemical entity is allowed to bind with the immobilized first chemical entity. The second chemical entity is or becomes coupled to a second fluorophore, which forms a FRET pair with the first fluorophore. The bound chemical entities are exposed to radiation at an excitation frequency for either the first or the second fluorophore, and polarization anisotropy of a FRET fluorescent signal from the bound chemical entities is measured to detect specific binding between the first and second chemical entities. Techniques are also disclosed for detecting whether a FRET interaction is occurring between a first chemical entity including a donor fluorophore and a second chemical entity including an acceptor fluorophore, using simultaneous anisotropy measurements at the wavelengths of the donor and acceptor fluorophores.
摘要翻译： 描述了用于检测第一和第二化学实体之间的特异性结合的方法和装置。 与第一荧光团相关联的第一化学实体被固定化。 允许第二化学实体与固定化的第一化学实体结合。 第二化学实体是或连接到与第一荧光团形成FRET对的第二荧光团。 结合的化学实体以第一或第二荧光团的激发频率暴露于辐射，并且测量来自结合的化学实体的FRET荧光信号的偏振各向异性以检测第一和第二化学实体之间的特异性结合。 还公开了用于检测在包括供体荧光团的第一化学实体和包括受体荧光团的第二化学实体之间是否发生FRET相互作用的技术，其使用在供体和受体荧光团的波长处的同时各向异性测量。
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公开(公告)号：US07141378B2
公开(公告)日：2006-11-28
申请号：US11173814
申请日：2005-07-01
CPC分类号： G01N33/582 , B82Y5/00 , B82Y10/00 , C12Q1/6816 , G01N21/6408 , G01N21/6428 , G01N21/6445 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6441 , G01N2021/6478 , G01N2021/6484 , G01N2201/067 , G01N2201/0697 , G01N2201/0833 , C12Q2563/107 , C12Q2561/119
摘要： Methods, apparatus, and system, implementing and using techniques for detecting a presence of one or more target analytes in particular regions of interest of one or more samples. One or more samples including objects and one or more target analytes are provided. Some of the target analytes are labeled with a fluorophore and are bound to some of the objects in the samples. The samples are illuminated with fluorescence inducing light and fluorescent light is collected from one or more regions of the one or more samples. At least one anisotropy measurement of the samples is performed to identify regions of interest where one or more target analytes are bound to the objects. The collected fluorescent light from the regions of interest is analyzed to determine a presence of target analytes that are bound to the objects in the one or more samples.
摘要翻译： 方法，装置和系统，实施和使用用于检测一个或多个样品的特定感兴趣区域中一种或多种目标分析物的存在的技术。 提供了一个或多个样品，包括物体和一种或多种目标分析物。 一些目标分析物用荧光团标记，并且与样品中的某些对象结合。 用荧光诱导光照射样品，并从一个或多个样品的一个或多个区域采集荧光。 进行样品的至少一个各向异性测量以识别一个或多个目标分析物与物体结合的感兴趣区域。 分析来自感兴趣区域的收集的荧光以确定与一个或多个样品中的物体结合的目标分析物的存在。
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公开(公告)号：US06254833B1
公开(公告)日：2001-07-03
申请号：US09134662
申请日：1998-07-30
IPC分类号： B01L300
CPC分类号： B01L3/5085 , B01J2219/00315 , B01J2219/00317 , B01J2219/00659 , B01J2219/00707 , B01J2219/0072 , B01L9/523 , B01L2300/0829 , C40B60/14 , G01N2021/0346 , Y10S435/968 , Y10S435/975
摘要： A microplate lid having a substantially flat, generally rectangular top with first and second pairs of opposite sides. A flange depends from the top of the first and second pairs of sides, the flange bearing at least one recess area along each side of the first pair of sides, the recessed area extending into the top.
摘要翻译： 一种微板盖，其具有基本平坦的，大致矩形的顶部，其具有第一和第二对相对侧。 凸缘从第一对侧面和第二对侧面的顶部开始，凸缘沿着第一对侧面的每一侧轴承至少一个凹部区域，凹进区域延伸到顶部。
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公开(公告)号：US5760299A
公开(公告)日：1998-06-02
申请号：US674131
申请日：1996-07-01
IPC分类号： G01N1/40 , G01N30/00 , G01N30/06 , G01N30/24 , G01N30/32 , G01N30/88 , G01N35/10 , G01N30/02
CPC分类号： G01N35/109 , G01N30/00 , G01N30/24 , G01N30/32 , G01N1/405 , G01N2030/009 , G01N2030/062 , G01N2030/324 , G01N2030/8804 , G01N2030/8886
摘要： A method and apparatus for solid phase extraction chromatography is disclosed in which the flow rate of a fluid, such as the motive pressurized gas is monitored, adjusted and compared with a standardized pressure curve which is correlative of a sample being processed. By monitoring and adjusting changes in flow rate, which can correspond to process initialization, beginning, ending and key fractionating points, and correlating to a standard, the cycle time for assaying the sample in question can be maximized and multiple samples can pass through analysis automatically and quickly.
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8.发明授权
公开(公告)号：US5531959A
公开(公告)日：1996-07-02
申请号：US381559
申请日：1994-01-31
IPC分类号： G01N1/40 , G01N30/00 , G01N30/06 , G01N30/24 , G01N30/32 , G01N30/88 , G01N35/10 , G01N30/02
CPC分类号： G01N35/109 , G01N30/00 , G01N30/24 , G01N30/32 , G01N1/405 , G01N2030/009 , G01N2030/062 , G01N2030/324 , G01N2030/8804 , G01N2030/8886
摘要： A method and apparatus for solid phase extraction chromatography is disclosed in which the flow rate of a fluid, such as the motive pressurized gas is monitored, adjusted and compared with a standardized pressure curve which is correlative of a sample being processed. By monitoring and adjusting changes in flow rate, which can correspond to process initialization, beginning, ending and key fractionating points, and correlating to a standard, the cycle time for assaying the sample in question can be maximized and multiple samples can pass through analysis automatically and quickly.
摘要翻译： 公开了一种用于固相萃取色谱法的方法和装置，其中监测，调节诸如动力加压气体的流体的流速，并与正在处理的样品相关的标准压力曲线进行比较。 通过监测和调整流量变化，可以对应于过程初始化，开始，结束和关键分馏点，并与标准相关，可以最大限度地提高测定样品的循环时间，多个样品可以自动通过分析 并快速
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