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公开(公告)号：US08773759B2
公开(公告)日：2014-07-08
申请号：US13121886
申请日：2009-09-22
IPC分类号： G02B21/00
CPC分类号： G02B21/006 , G02B21/0068 , G02B21/241 , G02B2207/114
摘要： A microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of the light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with at least one second mirror for transmitting light from the first objective lens in the direction of the second objective lens and from the second objective lens to the detector being disposed in the optical path, which second mirror is a fully reflective mirror, or a microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with a polarizing beam splitter for splitting the object light into two components that are oriented perpendicular to each other being disposed between the first and the second objective lens for light transmission.
摘要翻译： 一种具有用于调节焦距范围的装置的显微镜，包括用于在检测器的方向上传送被照射物体的物体的第一物镜，其中第二物镜沿检测器上游的光的方向设置， 第二物镜之后是可以在光轴方向上调节的第一反射镜，具有至少一个第二反射镜，用于在第二物镜的方向上从第一物镜透射光并从第二物镜传播光 所述检测器设置在所述光路中，所述第二反射镜是完全反射镜，或具有用于调节所述焦距的装置的显微镜，所述显微镜包括用于沿检测器的方向透射被照射物体的物体的第一物镜， 其中第二物镜沿着检测器上游的光的方向设置，该第二物镜后面是第一镜像 r可以在光轴的方向上调整，其中偏振分束器用于将物体光分成两个彼此垂直取向的分量，这两个分量被布置在第一和第二物镜之间用于透光。
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2.发明授权Method for imaging a sample using a microscope, and microscope and data storage center 有权使用显微镜对样品进行成像的方法，以及显微镜和数据存储中心公开(公告)号：US08294897B2
公开(公告)日：2012-10-23
申请号：US12576596
申请日：2009-10-09
申请人： Christopher Power
发明人： Christopher Power
IPC分类号： G01N21/25
CPC分类号： G02B21/0064 , G01N21/6458 , G02B21/0032 , G02B21/0076 , G02B21/008
摘要： The invention relates to a method for imaging a sample using a microscope, in particular a scanning microscope, in which the sample is illuminated with excitation light via an illuminating beam path, and light emitted from the sample is recorded via a detection beam path, wherein at least one adjustable beam splitter having an adjustable threshold wavelength is arranged in the detection beam path or/and in the illuminating beam path, and wherein light emitted from the sample is detected in at least one detection channel. According to the inventive method, for at least one predetermined sample region, a signal intensity of light detected in the at least one detection channel is recorded for a plurality of threshold wavelengths set at the adjustable beam splitter to obtain a signal/threshold-dependency of the predetermined sample region.
摘要翻译： 本发明涉及一种使用显微镜，特别是扫描显微镜对样品成像的方法，其中通过照明光束路径用激发光照射样品，并且经由检测光束路径记录从样品发射的光，其中 在检测光束路径中或/和照明光束路径中布置具有可调阈值波长的至少一个可调分束器，并且其中在至少一个检测通道中检测从样品发射的光。 根据本发明的方法，对于至少一个预定采样区域，在设置在可调分束器处的多个阈值波长中记录在至少一个检测通道中检测到的光的信号强度，以获得信号/阈值依赖性 预定的样品区域。
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公开(公告)号：US08228499B2
公开(公告)日：2012-07-24
申请号：US12682234
申请日：2008-09-18
CPC分类号： G02B21/34
摘要： The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.
摘要翻译： 本发明涉及将显影装置的样本空间中至少一个优选生物样本定位的方法以及用于实施这些方法的装置。 提出了相对于检测对象的光轴的试样的取向可以反复改变的方法和装置，并且在这样做的同时，保持试样，使得从每个检测方向确保了样本的基本无阻碍的视图。 在不同的结构变型中，试样通过粘合力或流动介质保持在支撑装置上，通过毛细管作用将样品保持在毛细管开口处，或者至少一个样品嵌入透明凝胶体中， 凝胶体通过可旋转保持装置固定在样本空间中，并且通过使保持装置旋转给定的旋转角度来改变检测方向。
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公开(公告)号：US09110301B2
公开(公告)日：2015-08-18
申请号：US13497996
申请日：2010-09-17
CPC分类号： G02B21/241 , G02B21/002 , G02B21/06
摘要： A family of microscopes with illumination systems directing a sheet of light having an approximately planar extension in an illumination axis of an illumination beam path and in a transverse axis orthogonally oriented to the illumination axis. The microscopes have detection devices used to detect light that is emitted by a sample region. The detection devices including a detection lens system disposed in the detection beam path and an optical detection element spaced from a front lens of the detection lens system and independently adjustable thereof. The optical detection element continuously varies the size of a detection image field and/or continuously displaces a focal plane of detection in the P-region.
摘要翻译： 具有照明系统的一系列显微镜，其在照明光束路径的照明轴线和垂直于照明轴线的横向轴线上引导具有近似平面延伸的光线。 显微镜具有用于检测由样品区域发射的光的检测装置。 检测装置包括设置在检测光束路径中的检测透镜系统和与检测透镜系统的前透镜间隔开的光学检测元件，并且可独立调节。 光检测元件连续地改变检测图像场的大小和/或连续地移位P区域中的检测焦平面。
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公开(公告)号：US08482854B2
公开(公告)日：2013-07-09
申请号：US12529051
申请日：2008-03-26
摘要： The invention is directed to a sample holder for a microscope. The sample holder comprises a sample chamber which is filled with an immersion liquid and in which a sample is located. The sample chamber has an upper opening. It further comprises means for translating the sample relative to a detection objective of the microscope, and means for rotating the sample around an axis of rotation extending in a substantially horizontal plane which encloses an angle other than zero degrees with the optical axis of the detection objective. In a sample holder of this kind, the sample is embedded in a transparent embedding medium having at least partially a greater solidity than the immersion liquid. Further, the sample chamber has means for horizontally supporting the embedded sample against the effect of gravity.
摘要翻译： 本发明涉及用于显微镜的样品架。 样品架包括样品室，其中填充有浸没液体并且样品位于样品室中。 样品室具有上部开口。 它还包括用于相对于显微镜的检测对象平移样本的装置，以及用于围绕围绕旋转轴旋转的装置的装置，该旋转轴在基本上水平的平面中旋转，该基本上水平的平面与检测目标的光轴 。 在这种样品保持器中，将样品嵌入至少部分具有比浸没液更大的固体的透明嵌入介质中。 此外，样品室具有用于水平地支撑嵌入样品免受重力影响的装置。
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6.发明授权Apparatus and method for high spatial resolution imaging of a structure of a sample 有权用于样品结构的高空间分辨率成像的装置和方法公开(公告)号：US08362448B2
公开(公告)日：2013-01-29
申请号：US12867291
申请日：2009-02-03
IPC分类号： G01J1/58
CPC分类号： G02B21/002 , G01N21/6458 , G02B21/10 , G02B27/58
摘要： Apparatus and method for high spatial resolution imaging of a sample's structure, including a diffraction-limited resolution volume with a plurality of dye molecules which can be switched between different states and have a distribution density which is greater than the inverse of the diffraction-limited resolution volume, where at least one state is fluorescing, the fluorescence being collected by an objective lens and imaged on a spatially resolving detector by an optical system. At least one light source provided for emitting a switching radiation and for emitting an excitation radiation. At least one of the light sources is arranged to radiate through the sample, and a switching and/or fluorescence excitation of the dye molecules is carried out. The switching is a photoactivation or a photodeactivation of the dye molecules. A focusing arrangement is provided for switching and/or for excitation to generate a line-like illumination region extending in a direction of illumination.
摘要翻译： 用于样品结构的高空间分辨率成像的装置和方法，包括具有多个染料分子的衍射限制分辨率体积，其可以在不同状态之间切换并且具有大于衍射受限分辨率的反比的分布密度 体积，其中至少一个状态是荧光的，荧光由物镜收集并通过光学系统在空间分辨检测器上成像。 提供用于发射切换辐射并用于发射激发辐射的至少一个光源。 至少一个光源布置成通过样品辐射，并且进行染料分子的切换和/或荧光激发。 切换是染料分子的光活化或光激活。 提供了用于切换和/或激发的聚焦装置，以产生在照明方向上延伸的线状照明区域。
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公开(公告)号：US08350230B2
公开(公告)日：2013-01-08
申请号：US12680265
申请日：2008-09-18
IPC分类号： G01N21/64
CPC分类号： G02B21/361 , G02B21/008 , G02B21/06
摘要： A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm.
摘要翻译： 一种用于分析样本的方法和装置，其中所述样本被支撑成可围绕旋转轴线旋转并且可在所有三个空间方向上移位并被第一照明装置照射。 从样品辐射的光在检测装置上成像。 在旋转角度的不同设定下记录试样的多个截面图像，并且使样本旋转。 记录的截面图像被融合以形成样本的空间图像数据的数据集。 然后通过垂直于旋转轴线的第二照明装置照射样本，其中记录样本的多个阴影图像并且样本旋转。 通过背投影算法从记录的阴影图像中构建样本的空间图像数据的第二数据集。
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公开(公告)号：US08213081B2
公开(公告)日：2012-07-03
申请号：US12596412
申请日：2008-04-04
IPC分类号： G02B21/02
CPC分类号： G02B21/248 , G02B21/33
摘要： The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same.
摘要翻译： 本发明涉及一种用于显微镜的目标替换装置，其中样品位于样品室中并被样品室内的浸没介质包围，用于相对于存在的物镜的焦点定位和对准样品的装置，其中 检测光束路径水平对准，即垂直于重力作用方向。 对于上述类型的显微镜的目标替换装置，根据本发明，提供了一种装置，用于在样品的焦点位置和对准位置处与至少另一个物体交换物镜的位置和对准 样品室内的样品保持不变。
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公开(公告)号：US20110031414A1
公开(公告)日：2011-02-10
申请号：US12937103
申请日：2009-04-04
IPC分类号： G01J1/58
CPC分类号： G02B21/16 , G02B21/002 , G02B21/06 , G02B21/10
摘要： A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.
摘要翻译： 提供了特别用于成像荧光寿命显微术方法的显微镜装置。 显微镜装置包括用于产生照明光束的照明装置，用于空间分辨地获取由待检查对象发射的发射辐射的成像检测器，照明装置和待检查对象之间的照射光束路径，以及检测 要检查的物体与检测器之间的光束路径。 照明光束路径包括照明光学器件，其设计成产生横向于照明光束路径的轴线延伸的照明辐射的光片，其中检测光束路径的轴线基本上垂直于光片的截面定向， 的被检查对象。 照明装置包括脉冲激光器。
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公开(公告)号：US20100309548A1
公开(公告)日：2010-12-09
申请号：US12680265
申请日：2008-09-18
IPC分类号： G02B21/06
CPC分类号： G02B21/361 , G02B21/008 , G02B21/06
摘要： A method and an arrangement for analyzing a specimen, wherein the specimen is supported so as to be rotatable around an axis of rotation and displaceable in all three spatial directions and is illuminated by a first illumination device. Light radiated from the specimen is imaged on a detection device. A plurality of sectional images of the specimen are recorded at different settings of the rotational angle, and the specimen is rotated. The recorded sectional images are fused to form a data set of spatial image data of the specimen. The specimen is then illuminated by a second illumination device perpendicular to the axis of rotation, wherein a plurality of shadow images of the specimen are recorded and the specimen is rotated. A second data set of spatial image data of the specimen is constructed from the recorded shadow images by means of a back projection algorithm.
摘要翻译： 一种用于分析样本的方法和装置，其中所述样本被支撑成可围绕旋转轴线旋转并且可在所有三个空间方向上移位并被第一照明装置照射。 从样品辐射的光在检测装置上成像。 在旋转角度的不同设定下记录试样的多个截面图像，并且使样本旋转。 记录的截面图像被融合以形成样本的空间图像数据的数据集。 然后通过垂直于旋转轴线的第二照明装置照射样本，其中记录样本的多个阴影图像并且样本旋转。 通过背投影算法从记录的阴影图像中构建样本的空间图像数据的第二数据集。
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