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公开(公告)号：US08506781B2
公开(公告)日：2013-08-13
申请号：US13555944
申请日：2012-07-23
Applicant: Craig Rowell , Cory Panattoni , Tom R Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
Inventor： Craig Rowell , Cory Panattoni , Tom R Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
IPC: G01N27/447 , C07K1/26
CPC classification number: G01N27/44747 , B01D57/02 , C08F220/56 , C08F222/385
Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。
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公开(公告)号：US08282800B2
公开(公告)日：2012-10-09
申请号：US12552104
申请日：2009-09-01
Applicant: Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
Inventor： Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
IPC: G01N27/447
CPC classification number: G01N27/44747 , B01D57/02 , C08F220/56 , C08F222/385
Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。
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公开(公告)号：US08945360B2
公开(公告)日：2015-02-03
申请号：US12691440
申请日：2010-01-21
Applicant: Shane Petersen , Cory Panattoni , Craig Rowell
Inventor： Shane Petersen , Cory Panattoni , Craig Rowell
IPC: C07K1/26 , G01N27/447
CPC classification number: G01N27/44747 , C07K1/26 , Y10S436/826
Abstract: Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.
Abstract translation: 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。
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公开(公告)号：US20120285830A1
公开(公告)日：2012-11-15
申请号：US13555944
申请日：2012-07-23
Applicant: Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
Inventor： Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
IPC: C07K1/26
CPC classification number: G01N27/44747 , B01D57/02 , C08F220/56 , C08F222/385
Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。
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公开(公告)号：US08128799B2
公开(公告)日：2012-03-06
申请号：US12437698
申请日：2009-05-08
Applicant: Glenn Price , Cory Panattoni , Craig Rowell , Shane Petersen , Evelio Perez
Inventor： Glenn Price , Cory Panattoni , Craig Rowell , Shane Petersen , Evelio Perez
CPC classification number: G01N27/453 , G01N27/44704 , G01N27/44756
Abstract: A slab gel cassette is formed from generally flat plates welded together along their borders with a gel space between the plates that is open at the top and bottom edges of the plates to expose the opposing ends of a gel in the gel space to buffer solutions in which electrodes are immersed. The plates are shaped such that the weld line does not intersect with the bottom opening of the gel space. Sealing of the bottom of the gel space, which is necessary for gel casting, is achieved by placing a sealing strip over the bottom opening with no danger of leakage of the gel solution along a weld line.
Abstract translation: 板状凝胶盒由大致平板形成，沿着其边界焊接在一起，板之间的凝胶空间在板的顶部和底部边缘处敞开，以露出凝胶空间中的凝胶的相对端，以缓冲溶液 哪些电极被浸没 板的形状使得焊接线不与凝胶空间的底部开口相交。 通过将密封条放置在底部开口上而没有凝胶溶液沿着焊接线泄漏的危险来实现凝胶浇铸所需的凝胶空间的底部的密封。
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公开(公告)号：US20100187114A1
公开(公告)日：2010-07-29
申请号：US12691440
申请日：2010-01-21
Applicant: Shane Petersen , Cory Panattoni , Craig Rowell
Inventor： Shane Petersen , Cory Panattoni , Craig Rowell
IPC: C07K1/26 , G01N27/447
CPC classification number: G01N27/44747 , C07K1/26 , Y10S436/826
Abstract: Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.
Abstract translation: 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。
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公开(公告)号：US20100051462A1
公开(公告)日：2010-03-04
申请号：US12552104
申请日：2009-09-01
Applicant: Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
Inventor： Craig Rowell , Cory Panattoni , Thomas R. Berkelman , Sean Cater , Shane Petersen , Lee Olech , Xuemei Yang
IPC: G01N33/559 , B01D57/02
CPC classification number: G01N27/44747 , B01D57/02 , C08F220/56 , C08F222/385
Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.
Abstract translation: 聚丙烯酰胺凝胶在蛋白质分离中提供高分辨率并且相对于水解而言比常规聚丙烯酰胺凝胶更稳定，其依赖于Tris或Tris-Bis作为缓冲剂，通过掺入三乙醇胺代替大部分或全部的Tris或Tris-Bis制备。
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公开(公告)号：US20090308751A1
公开(公告)日：2009-12-17
申请号：US12437698
申请日：2009-05-08
Applicant: Glenn Price , Cory Panattoni , Craig Rowell , Shane Petersen , Evelio Perez
Inventor： Glenn Price , Cory Panattoni , Craig Rowell , Shane Petersen , Evelio Perez
IPC: G01N27/447
CPC classification number: G01N27/453 , G01N27/44704 , G01N27/44756
Abstract: A slab gel cassette is formed from generally flat plates welded together along their borders with a gel space between the plates that is open at the top and bottom edges of the plates to expose the opposing ends of a gel in the gel space to buffer solutions in which electrodes are immersed. The plates are shaped such that the weld line does not intersect with the bottom opening of the gel space. Sealing of the bottom of the gel space, which is necessary for gel casting, is achieved by placing a sealing strip over the bottom opening with no danger of leakage of the gel solution along a weld line.
Abstract translation: 板状凝胶盒由大致平板形成，沿着其边界焊接在一起，板之间的凝胶空间在板的顶部和底部边缘处敞开，以露出凝胶空间中的凝胶的相对端，以缓冲溶液 哪些电极被浸没 板的形状使得焊接线不与凝胶空间的底部开口相交。 通过将密封条放置在底部开口上而没有凝胶溶液沿着焊接线泄漏的危险来实现凝胶浇铸所需的凝胶空间的底部的密封。
