公开(公告)号：US09611317B2

公开(公告)日：2017-04-04

申请号：US13897843

申请日：2013-05-20

申请人： Crucell Holland B.V.

发明人： Mark Throsby ,  Robert H. E. Friesen ,  Theodorus H. J. Kwaks ,  Mandy A. C. Jongeneelen

IPC分类号： A61K39/145 ,  C12Q1/70 ,  C07K16/10 ,  A61K39/12 ,  A61K39/00

CPC分类号： C07K16/1018 ,  A61K39/12 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2039/545 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2317/92 ,  C12N2760/16111

摘要： Described are binding molecules, e.g., human monoclonal antibodies, that bind to influenza virus comprising HA of the H3 subtype, e.g., H3N2, and have a broad neutralizing activity against such influenza virus. Described are polynucleotides encoding the binding molecules, their sequences and compositions comprising the binding molecules and methods of identifying or producing the binding molecules. The binding molecules can be used in the diagnosis, prophylaxis, and/or treatment of influenza virus H3N2 infection. The binding molecules may provide cross-subtype protection, such that infections with H3, H7, and/or H10-based influenza subtypes can be prevented and/or treated.

公开(公告)号：US09428572B2

公开(公告)日：2016-08-30

申请号：US14101584

申请日：2013-12-10

申请人： Crucell Holland B.V.

发明人： Mark Throsby ,  Robert A. Kramer ,  Cornelis A. de Kruif

IPC分类号： A61K39/00 ,  A61K39/40 ,  C07K16/00 ,  C12P21/08 ,  C07K16/12 ,  G01N33/569 ,  A61K38/00 ,  C07K16/18

CPC分类号： C07K16/1271 ,  A61K38/00 ,  A61K39/40 ,  A61K2039/505 ,  C07K16/1267 ,  C07K16/18 ,  C07K2317/21 ,  G01N33/56911

摘要： Described are human binding molecules specifically binding to enterococci and having killing activity against enterococci, nucleic acid molecules encoding the human binding molecules, compositions comprising the human binding molecules and methods of identifying or producing the molecules. The molecules can be used, for example, in the diagnosis, prophylaxis, and/or treatment of a condition resulting from Enterococcus.

摘要翻译： 描述了特异性结合肠球菌并具有针对肠球菌的杀伤活性的人结合分子，编码人结合分子的核酸分子，包含人结合分子的组合物以及鉴定或产生分子的方法。 分子可以用于例如肠球菌引起的病症的诊断，预防和/或治疗。

公开(公告)号：US20160102123A1

公开(公告)日：2016-04-14

申请号：US14786955

申请日：2014-04-24

申请人： Crucell Holland B.V.

发明人： Johannes Petrus Maria Langedijk ,  Anders Krarup

IPC分类号： C07K14/005 ,  C12N7/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/155 ,  A61K2039/55561 ,  C07K14/135 ,  C07K2319/00 ,  C07K2319/70 ,  C07K2319/73 ,  C12N7/00 ,  C12N2760/18522 ,  C12N2760/18534

摘要： Described are stable pre-fusion respiratory syncitial virus (RSV) F polypeptides, immunogenic compositions comprising the polypeptides and uses thereof for the prevention and/or treatment of RSV infection.

摘要翻译： 描述了稳定的融合前呼吸道合胞体病毒（RSV）F多肽，包含多肽的免疫原性组合物及其用于预防和/或治疗RSV感染的用途。

公开(公告)号：US09022240B2

公开(公告)日：2015-05-05

申请号：US14275392

申请日：2014-05-12

申请人： Crucell Holland B.V.

发明人： John A. Lewis

IPC分类号： C12N7/00 ,  A61K39/13

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/13 ,  C12N2770/32634 ,  C12N2770/32651 ,  Y02A50/466

摘要： Described is a process for producing poliovirus, the process comprising: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.

摘要翻译： 描述了产生脊髓灰质炎病毒的方法，该方法包括：a）提供通过腺病毒E1序列表达而永生化的原代人视网膜（HER）细胞的细胞的无血清悬浮培养物，b）用 脊髓灰质炎病毒，细胞密度在2×10 6个细胞/ ml和150×10 6个细胞/ ml之间，和c）在感染后12至48小时之间收获脊髓灰质炎病毒。

公开(公告)号：US20140357845A1

公开(公告)日：2014-12-04

申请号：US14361281

申请日：2012-11-27

申请人： Crucell Holland B.V.

发明人： Jan Willem Meijberg ,  Atonietta Impagliazzo ,  Ronald Vogels ,  Robert H.E. Friesen ,  Philippe Alard ,  Stefan Loverix ,  Katarina Radosevic

IPC分类号： A61K39/145 ,  C07K14/005 ,  C12N7/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5258 ,  A61K2039/55522 ,  A61K2039/575 ,  C07K16/1018 ,  C07K2317/33 ,  C07K2317/76 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/40 ,  C07K2319/50 ,  C07K2319/73 ,  C12N7/00 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16171

摘要： The present disclosure provides influenza hemagglutinin stem domain polypeptides comprising (a) an influenza hemagglutinin HA1 domain that comprises an HA1 N-terminal stem segment, covalently linked by a linking sequence of 0-50 amino acid residues to an HA1 C-terminal stem segment, and (b) an influenza hemagglutinin HA2 domain, wherein on or more amino acids in the HA2 domain have been mutated. Also provided are nucleic acids encoding the polypeptides, compositions comprising the polypeptides and/or nucleic acid molecules, as well as methods of their use, in particular in the detection, prevention and/or treatment of influenza.

摘要翻译： 本公开提供了流感血凝素干结构域多肽，其包含（a）流感血凝素HA1结构域，其包含通过0-50个氨基酸残基的连接序列与HA1 C-末端茎段共价连接的HA1N末端茎段， 和（b）流感血凝素HA2结构域，其中在HA2结构域中的多个氨基酸已被突变。 还提供了编码多肽的核酸，包含多肽和/或核酸分子的组合物，以及它们用途的方法，特别是在流感的检测，预防和/或治疗中。

公开(公告)号：US20140341929A1

公开(公告)日：2014-11-20

申请号：US14323908

申请日：2014-07-03

申请人： Crucell Holland B.V.

发明人： Ronald Vogels ,  Theodorus H.J. Kwaks

IPC分类号： C07K16/10 ,  A61K47/48 ,  G01N33/569 ,  A61K39/42

CPC分类号： C07K16/1018 ,  A61K39/42 ,  A61K47/6841 ,  A61K2039/505 ,  C07K16/10 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/76 ,  G01N33/56983 ,  G01N2333/11

摘要： Described are binding molecules, such as human monoclonal antibodies, that bind to hemagglutinin of influenza B viruses, and have a broad neutralizing activity against such influenza viruses. These binding molecules do not bind to hemagglutinin of influenza A viruses. Further provided are nucleic acid molecules encoding the binding molecules, and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis of, prophylaxis against, and/or treatment of influenza B virus infections.

公开(公告)号：US20140242670A1

公开(公告)日：2014-08-28

申请号：US14275392

申请日：2014-05-12

申请人： Crucell Holland B.V.

发明人： John A. Lewis

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/13 ,  C12N2770/32634 ,  C12N2770/32651 ,  Y02A50/466

摘要： Described is a process for producing poliovirus, the process comprising: a) providing a serum-free suspension culture of cells, which are primary human retina (HER) cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.

摘要翻译： 描述了产生脊髓灰质炎病毒的方法，该方法包括：a）提供通过腺病毒E1序列表达而永生化的原代人视网膜（HER）细胞的细胞的无血清悬浮培养物，b）用 脊髓灰质炎病毒，细胞密度在2×10 6个细胞/ ml和150×10 6个细胞/ ml之间，和c）在感染后12至48小时之间收获脊髓灰质炎病毒。

公开(公告)号：US20130273106A1

公开(公告)日：2013-10-17

申请号：US13843052

申请日：2013-03-15

申请人： Crucell Holland B.V.

发明人： John A. Lewis

IPC分类号： C12N7/00

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/13 ,  C12N2770/32634 ,  C12N2770/32651 ,  Y02A50/466

摘要： Described is a process for producing poliovirus, the process comprising: a) providing a serum-free suspension culture of cells, which are primary human retina cells that have been immortalized by expression of adenovirus E1 sequences, b) infecting the cells with poliovirus, at a cell density of between 2×106 cells/ml and 150×106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.

摘要翻译： 描述了产生脊髓灰质炎病毒的方法，该方法包括：a）提供细胞的无血清悬浮培养物，其是通过腺病毒E1序列表达而永生化的原代人视网膜细胞，b）用脊髓灰质炎病毒感染细胞， 细胞密度在2×10 6细胞/ ml和150×10 6个细胞/ ml之间，c）在感染后12和48小时之间收获脊髓灰质炎病毒。

公开(公告)号：US20130243792A1

公开(公告)日：2013-09-19

申请号：US13789284

申请日：2013-03-07

申请人： CRUCELL HOLLAND B.V.

发明人： Ronald Vogels ,  Theodorus H.J. Kwaks

IPC分类号： C07K16/10

CPC分类号： C07K16/1018 ,  A61K39/42 ,  A61K47/6841 ,  A61K2039/505 ,  C07K16/10 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2317/76 ,  G01N33/56983 ,  G01N2333/11

摘要： Described are binding molecules, such as human monoclonal antibodies, that bind to hemagglutinin of influenza B viruses, and have a broad neutralizing activity against such influenza viruses. These binding molecules do not bind to hemagglutinin of influenza A viruses. Further provided are nucleic acid molecules encoding the binding molecules, and compositions comprising the binding molecules. The binding molecules can be used in the diagnosis of, prophylaxis against, and/or treatment of influenza B virus infections.

公开(公告)号：US10196438B2

公开(公告)日：2019-02-05

申请号：US14784937

申请日：2014-04-14

申请人： Crucell Holland B.V.

发明人： Jehangir Wadia ,  Robert Anthony Williamson ,  Johannes P. M. Langedijk ,  Gabriel Pascual ,  Angelique Van 'T Wout

IPC分类号： C07K14/115 ,  A61K39/155 ,  A61K39/145 ,  C07K14/005 ,  C07K16/10 ,  G01N33/569 ,  A61K47/68 ,  A61K39/00

摘要： The disclosure relates to isolated antibodies and antigen-binding fragments that bind to the G protein of RSV and which are capable of neutralizing RSV A and B subtypes, and the use thereof in the diagnosis, prophylaxis, and/or treatment of RSV infections.