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    Method for selectively separating blood cells by using lectin
    1.
    发明申请
    Method for selectively separating blood cells by using lectin 审中-公开
    通过使用凝集素选择性分离血细胞的方法

    公开(公告)号：US20050032201A1

    公开(公告)日：2005-02-10

    申请号：US10936937

    申请日：2004-09-09

    申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  C12M1/34

    CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

    摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are present in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

    摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的存在量，即使从固相中回收的细胞与固相相结合， 聚合物，从液相中回收的细胞不与聚合物结合固相。

    AGENT FOR REDUCTION OF BLEEDING IN CEREBROVASCULAR DISORDER
    2.
    发明申请
    AGENT FOR REDUCTION OF BLEEDING IN CEREBROVASCULAR DISORDER 审中-公开
    用于减少脑血管疾病发生的代理

    公开(公告)号：US20090226412A1

    公开(公告)日：2009-09-10

    申请号：US11993917

    申请日：2006-06-23

    申请人： Tetsuya Yasuhiro ,  Takashi Kitajima ,  Daisuke Wakamatsu ,  Wataru Kamoshima

    发明人： Tetsuya Yasuhiro ,  Takashi Kitajima ,  Daisuke Wakamatsu ,  Wataru Kamoshima

    IPC分类号： A61K38/49 ,  C07D413/12 ,  A61K31/5377 ,  A61P7/04

    CPC分类号： A61K45/06 ,  A61K31/5377

    摘要： The present invention relates to a hemorrhage reducing agent in cerebrovascular disorder containing a poly (ADP-ribose) polymerase inhibitor (PARP inhibitor). The PARP inhibitor provides an inhibitory effect of vascular endothelial cell disorder so that it may reduce hemorrhage in cerebrovascular disorder. In addition, the PARP inhibitor inhibits the hemorrhage that is concerned about in thrombolytic agent use by using together with a thrombolytic agent, and an effect of extending therapeutic time window of a thrombolytic agent may be further expected. Furthermore, the PARP inhibitor can be a safe hemorrhage reducing agent with fewer side effects because it does not affect the blood coagulation system and the fibrinolytic system.

    摘要翻译： 本发明涉及含有聚（ADP-核糖）聚合酶抑制剂（PARP抑制剂）的脑血管障碍的出血减少剂。 PARP抑制剂提供血管内皮细胞病症的抑制作用，以减少脑血管障碍的出血。 此外，PARP抑制剂通过与血栓溶解剂一起使用来抑制血栓溶解剂使用中涉及的出血，并且可以进一步预期延长溶栓剂的治疗时间窗口的效果。 此外，PARP抑制剂可以是具有较少副作用的安全性出血减少剂，因为它不影响凝血系统和纤维蛋白溶解系统。

    Method for selectively separating blood cells by using lectin
    3.
    发明授权
    Method for selectively separating blood cells by using lectin 有权
    通过使用凝集素选择性分离血细胞的方法

    公开(公告)号：US06926915B1

    公开(公告)日：2005-08-09

    申请号：US09937510

    申请日：2000-03-30

    申请人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    发明人： Hirofumi Yura ,  Yoshio Saito ,  Michihiro Kitagawa ,  Daisuke Wakamatsu ,  Akira Ihara

    IPC分类号： C12N5/00 ,  C12N5/078 ,  G01N33/569 ,  A61K35/78

    CPC分类号： G01N33/56972 ,  C12N5/0634 ,  G01N33/56966 ,  G01N2333/4724

    摘要： The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes/non-complexes are formed, the sample containing these cell-lectin complexes/non-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.

    摘要翻译： 本发明提供从血液样品中选择性和高产率分离，浓缩和回收所需细胞的方法。 本发明的方法的特征在于使血液样品与细胞膜无活性且细胞凝集素复合物/非复合物形成的条件下的凝集素相互作用，含有这些细胞凝集素复合物/非 - - 复合物与底物一起孵育，其表面被聚合物覆盖，所述聚合物具有由凝集素特异性识别的碳水化合物链，并且细胞通过凝集素固定在基质的表面上，随后将液体层和 分离固相，从液相和/或固相中回收所需的血细胞，这些凝集素的量使得尽管从固相中回收的细胞与固相结合， 从液相中回收的细胞不与聚合物结合固相。