公开(公告)号：US08414773B2

公开(公告)日：2013-04-09

申请号：US12348305

申请日：2009-01-04

申请人： Dale A. Davison ,  Ruth A. Pipes ,  Jack E. Silver

发明人： Dale A. Davison ,  Ruth A. Pipes ,  Jack E. Silver

IPC分类号： B01D15/08

CPC分类号： B01D15/10 ,  B01D15/16 ,  B01J20/28019 ,  B01J20/283 ,  G01N30/16 ,  G01N35/00871 ,  G01N2030/0095

摘要： Sample is applied to a chromatographic column containing shape stabilized packing and the liquid phase is pumped through the shape stabilized packing at a rate of flow greater than the standard rate of flow to obtain at least one of a target resolution and target time of run. Because of the improved resolution provided by the shape stabilized packing at higher rates of flow of solvent, the chromatographer can either obtain better resolution with a standard gradient or shorten the time of the run and reduce the amount of solvent needed by increasing the slope of the gradient.

摘要翻译： 将样品应用于含有形状稳定的填料的色谱柱，并且液相以大于标准流速的流速泵送通过形状稳定的填料，以获得目标分辨率和目标运行时间中的至少一个。 由于在更高的溶剂流速下由形状稳定的填料提供的改进的分辨率，色谱仪可以以标准梯度获得更好的分辨率或缩短运行时间，并通过增加斜率的斜率来减少所需的溶剂量 梯度。

公开(公告)号：US20080047899A1

公开(公告)日：2008-02-28

申请号：US11508812

申请日：2006-08-23

申请人： Dale A. Davison ,  Mikael E. Mahler ,  Veronica D. Thomason ,  John J. Urh ,  David Trail

发明人： Dale A. Davison ,  Mikael E. Mahler ,  Veronica D. Thomason ,  John J. Urh ,  David Trail

IPC分类号： B01D15/08

CPC分类号： G01N30/90 ,  B01D15/165 ,  B01D15/166 ,  G01N30/91

摘要： An isocratic gradient profile is inserted into a gradient profile during a flash chromatographic run when TLC indicates that it will be difficult to separate the component being purified from its closest impurity by a gradient. TLC is utilized to determine at least two retention factors with two significantly different solvent strengths for the same solvent system. The two or more retention factors are used to determine a solvent strength in which the retention factor of a target component and the retention factor of a closest impurity are within 0.8 of each other. The isocratic gradient profile is started when this solvent strength is reached during the gradient chromatographic run. It is ended when the earlier of four events occurs, which are: (1) the end of a second peak if a first peak is detected at an isocratic-gradient profile starting-solvent strength or within a predetermined starting tolerance of the isocratic-gradient profile starting-solvent strength detection; (2) the end of the first peak after the starting tolerance; (3) the detection of a peak during the isocratic gradient profile or isocratic segment run after the regular isocratic time period; or (4) an operator initiated termination of the isocratic gradient profile or isocratic segment run. The gradient profile then resumes and continues to the end of the run.

摘要翻译： 在快速色谱运行期间，将等度梯度曲线插入梯度曲线，当TLC指示将难以将被纯化的组分与其最接近的杂质分离一个梯度时。 使用TLC来确定相同溶剂系统具有两个显着不同的溶剂强度的至少两个保留因子。 使用两个或更多个保留因子来确定其中目标组分的保留因子和最接近的杂质的保留因子彼此在0.8内的溶剂强度。 当梯度色谱运行期间达到溶剂强度时，开始等度梯度曲线。 当发生四个事件的早期时，结束，其是：（1）如果以等度梯度曲线起始溶剂强度或在等度梯度的预定起始公差内检测到第一峰，则第二峰的结束 轮廓起始溶剂强度检测; （2）起始公差后第一个峰值的结束; （3）在等度梯度曲线或等度段在正常等时时间段后运行的峰值的检测; 或（4）运营商开始终止等度梯度分布或等度分段运行。 渐变轮廓然后恢复并继续到运行结束。

公开(公告)号：US07008541B2

公开(公告)日：2006-03-07

申请号：US10697496

申请日：2003-10-30

申请人： Delwin D. Hodgin ,  Dale A. Davison

发明人： Delwin D. Hodgin ,  Dale A. Davison

IPC分类号： B01D15/08

CPC分类号： G01N30/6052 ,  B01D15/22 ,  G01N30/6004 ,  Y10T29/49604

摘要： To make an inexpensive chromatographic column and perform chromatography with it, column walls and a column end with a port are molded integrally from plastic. A closure is integrally molded with a port as well. One type of closure includes part of a snap-on fastener integrally molded to it to cooperate with corresponding parts molded integrally with the column walls and another type of closure is welded to the tubular walls. In the preferred embodiment the welding is spin welding. The end and closure have channels molded into them radiating from a port and opening toward packing material such as silica beads or porous polymeric plugs. Filters and secondary seals may be located at the ends to prevent leakage of packing material.

摘要翻译： 为了制备便宜的色谱柱并进行色谱，柱状壁和带端口的柱端部由塑料一体成型。 封闭件也与端口一体模制。 一种类型的封闭件包括一体地模制到其上的卡扣紧固件的一部分，以与与柱壁一体模制的相应部件配合，并且另一种类型的封闭件焊接到管状壁。 在优选实施例中，焊接是旋转焊接。 端部和封闭件具有模制成从其中的通道的通道和开口朝向诸如二氧化硅珠或多孔聚合物塞的包装材料。 过滤器和二次密封件可以位于端部以防止包装材料泄漏。

公开(公告)号：US06949194B2

公开(公告)日：2005-09-27

申请号：US10389626

申请日：2003-03-14

申请人： Delwin D. Hodgin ,  Dale A. Davison

发明人： Delwin D. Hodgin ,  Dale A. Davison

IPC分类号： B01D15/22 ,  G01N30/60 ,  B01D15/08

CPC分类号： B01D15/22 ,  G01N30/6004 ,  G01N30/6052 ,  G01N30/6091 ,  Y10T29/49604 ,  Y10T436/255

摘要： To make an inexpensive chromatographic column and perform chromatography with it, column walls and a column end with an inlet port are molded integrally from plastic and a snap-on end is molded integrally with an outlet port from plastic. A filter is placed in one end of the column and the column is filled with packing material up to the second end. A filter is placed on top of the packing and a snap-on end with a molded outlet port is snapped onto the body with linear motion until an interference fit is formed. As the body and snap-on end are moved together cantilever members formed integrally with the snap on end are bent outwardly by detent members until the detent members are captured by an opening in the cantilever members.

摘要翻译： 为了制造便宜的色谱柱并进行色谱，柱状壁和具有入口的柱端部由塑料一体成型，并且卡扣端与塑料的出口一体地模制。 过滤器放置在塔的一端，柱子填充有直到第二端的填料。 将过滤器放置在填料的顶部，具有模制出口的卡扣端以线性运动卡扣在主体上，直到形成过盈配合。 随着主体和卡扣端部一起移动，与卡扣端部一体形成的悬臂部件通过制动部件向外弯曲，直到止动件被悬臂部件中的开口捕获。

公开(公告)号：US06904784B2

公开(公告)日：2005-06-14

申请号：US10082710

申请日：2002-02-25

申请人： Robert W. Allington ,  Dale A. Davison ,  Scott L. Blakley

发明人： Robert W. Allington ,  Dale A. Davison ,  Scott L. Blakley

IPC分类号： B01D15/12 ,  B01D15/16 ,  B01D15/24 ,  G01N1/00 ,  G01N1/38 ,  G01N30/02 ,  G01N30/22 ,  G01N30/24 ,  G01N30/32 ,  G01N30/46 ,  G01N30/74 ,  G01N30/88

CPC分类号： G01N30/466 ,  B01D15/12 ,  B01D15/166 ,  B01D15/247 ,  G01N1/14 ,  G01N1/38 ,  G01N30/22 ,  G01N30/24 ,  G01N30/32 ,  G01N30/34 ,  G01N30/74 ,  G01N30/88 ,  G01N35/1095 ,  G01N35/1097 ,  G01N2001/383 ,  G01N2030/027 ,  G01N2030/324 ,  G01N2030/326 ,  G01N2030/347

摘要： To economically perform preparatory chromatography, a plurality of pumps each having a corresponding one of a plurality of pistons and a corresponding one of a plurality of cylinders are driven by one motor to draw and pump solvent simultaneously into corresponding columns. To form a gradient, the pumps are connected to two-way valves that are connected alternately to a first solvent and a second solvent, whereby the time said valve is in a first position controls the amount of solvent drawn from the first reservoir into said pumps and the amount of time in said second position controls the amount of said second solvent drawn from the second reservoir into said pumps and the solvent is mixed in the pumping systems. The detectors are photodiodes mounted to light guides in the flow cells that generate signals related to light absorbance and communicate with a controller, whereby the controller receives signals indicating solute between the light guides and causes collection of solute. An over-pressure system compensates for pressure over a predetermined level.

摘要翻译： 为了经济地进行准备色谱，每个具有多个活塞中的相应一个活塞和多个气缸中的相应一个的多个泵由一个电动机驱动，以将溶剂同时泵送到相应的列中。 为了形成梯度，泵连接到交替地连接到第一溶剂和第二溶剂的二通阀，由此所述阀处于第一位置的时间控制从第一储存器抽吸到所述泵中的溶剂的量 并且所述第二位置中的时间量将从第二储存器抽出的所述第二溶剂的量控制在所述泵中，并且溶剂在泵送系统中混合。 检测器是安装在流通池中的光导的光电二极管，其产生与吸光度相关的信号并与控制器通信，由此控制器接收指示光导之间的溶质并引起溶质的收集的信号。 过压系统补偿超过预定水平的压力。

公开(公告)号：US20120205314A1

公开(公告)日：2012-08-16

申请号：US13207948

申请日：2011-08-11

申请人： Dale A. Davison

发明人： Dale A. Davison

IPC分类号： B01D15/08 ,  B01D15/10 ,  B01D15/42

CPC分类号： B01D15/166 ,  G01N30/34

摘要： To provide or maintain pump prime in a liquid chromatographic system when changing solvents or solvent reservoirs or starting up a chromatographic run, first and second solvents are supplied to a mixer through corresponding first and second lines and from the mixer to a chromatographic column. Air in one of the lines is removed by a pump and the line is filled with solvent.

摘要翻译： 在更换溶剂或溶剂储存器或启动色谱运行时，为了在液相色谱系统中提供或维持泵浦原料，将第一和第二溶剂通过相应的第一条和第二条管线从混合器提供给色谱柱。 其中一条线路中的空气通过泵除去，管线中充满溶剂。

公开(公告)号：US20120204626A1

公开(公告)日：2012-08-16

申请号：US13207124

申请日：2011-08-10

申请人： Dale A. Davison

发明人： Dale A. Davison

IPC分类号： G01N30/84

CPC分类号： G01N30/16 ,  B01D15/14 ,  G01N30/18 ,  G01N30/20 ,  G01N2030/167

摘要： A sample injection apparatus includes a sample container and a tube that communicates with the inlet of a liquid chromatographic column. The sample container receives at least a preparatory-sized sample. A syringe pump pulls sample from the sample container and applies it to the inlet of the liquid chromatographic column.

摘要翻译： 样品注射装置包括样品容器和与液相色谱柱的入口连通的管。 样品容器至少接收准备大小的样品。 注射器泵从样品容器中抽取样品并将其施加到液相色谱柱的入口。

公开(公告)号：US20090056541A1

公开(公告)日：2009-03-05

申请号：US12228725

申请日：2008-08-15

申请人： Dale A. Davison ,  Ruth Pipes ,  Jack E. Silver

发明人： Dale A. Davison ,  Ruth Pipes ,  Jack E. Silver

IPC分类号： B01D53/02 ,  B01D15/08 ,  C07F7/02

CPC分类号： B01D15/325 ,  B01D15/14 ,  B01D15/163 ,  B01D15/20 ,  B01D15/22 ,  B01D15/3861 ,  B01J20/28004 ,  B01J20/28019 ,  B01J20/286 ,  B01J20/287 ,  G01N30/603 ,  G01N30/6052 ,  G01N30/6091 ,  G01N30/6004

摘要： To make an inexpensive chromatographic column and perform chromatography with it, column walls and a column end with a port are molded integrally from plastic. A closure is integrally molded with a port as well. One type of closure includes part of a snap-on fastener integrally molded to it to cooperate with corresponding parts molded integrally with the column walls. In another type of closure for higher pressures, the closure is spin welded to the tubular walls. In still another type of closure for still higher pressures, a retaining plate is pressed into the column to hold the packing in place. The closures have channels molded into them radiating from a port and opening toward packing material such as silica beads or porous polymeric plugs. Filters and secondary seals may be located at the ends to prevent leakage of the packing material. The packing is balanced spherical or spheroid-like derivitized silica packing.

摘要翻译： 为了制备便宜的色谱柱并进行色谱，柱状壁和带端口的柱端部由塑料一体成型。 封闭件也与端口一体模制。 一种类型的封闭件包括一体地模制到其上的卡扣紧固件的一部分，以与与柱壁一体模制的相应部件配合。 在用于更高压力的另一种类型的封闭件中，封闭件旋转焊接到管状壁。 在仍然更高压力的另一种类型的封闭件中，将保持板压入柱中以将填料保持在适当位置。 封闭件具有模制成它们的通道，其从端口辐射并向包装材料（例如二氧化硅珠或多孔聚合物塞）开口。 过滤器和二次密封件可以位于端部以防止包装材料泄漏。 包装是平衡的球形或类球状衍生二氧化硅填料。

公开(公告)号：US06565745B2

公开(公告)日：2003-05-20

申请号：US09920124

申请日：2001-08-01

申请人： Delwin D. Hodgin ,  Dale A. Davison

发明人： Delwin D. Hodgin ,  Dale A. Davison

IPC分类号： B01D1508

CPC分类号： B01D15/22 ,  G01N30/6004 ,  G01N30/6052 ,  G01N30/6091 ,  Y10T29/49604 ,  Y10T436/255

摘要： To make an inexpensive chromatographic column and perform chromatography with it, column walls and a column end with an inlet port are molded integrally from plastic and a snap-on end is molded integrally with an outlet port from plastic. A filter is placed in one end of the column and the column is filled with packing material up to the second end. A filter is placed on top of the packing and a snap-on end with a molded outlet port is a snapped onto the body with linear motion until an interference fit is formed. As the body and snap-on end are moved together cantilever members formed integrally with the snap on end are bent outwardly by detent members until the detent members are captured by an opening in the cantilever members.

摘要翻译： 为了制造便宜的色谱柱并进行色谱，柱状壁和具有入口的柱端部由塑料一体成型，并且卡扣端与塑料的出口一体地模制。 过滤器放置在塔的一端，柱子填充有直到第二端的填料。 过滤器放置在填料的顶部，具有模制的出口的卡扣端以线性运动卡扣在主体上，直到形成过盈配合。 随着主体和卡扣端部一起移动，与卡扣端部一体形成的悬臂部件通过制动部件向外弯曲，直到止动件被悬臂部件中的开口捕获。

公开(公告)号：US5755559A

公开(公告)日：1998-05-26

申请号：US673177

申请日：1996-06-26

申请人： Robert William Allington ,  Daniel Gene Jameson ,  Dale A. Davison ,  Dale Clay ,  Robin R. Winter ,  Yoossef Tehrani

发明人： Robert William Allington ,  Daniel Gene Jameson ,  Dale A. Davison ,  Dale Clay ,  Robin R. Winter ,  Yoossef Tehrani

IPC分类号： B01D11/00 ,  B01D11/02 ,  B01D11/04 ,  F04B9/04 ,  F04B15/08 ,  F04B49/06 ,  F04B53/08 ,  F04B53/14 ,  F16K15/18 ,  G01F1/34 ,  G01N1/34 ,  G01N1/40 ,  G01N30/02 ,  G01N30/28 ,  G01N30/60 ,  G01N35/04 ,  G21F5/015 ,  F04B49/00

CPC分类号： G01N1/34 ,  B01D11/0203 ,  B01D11/0407 ,  F04B15/08 ,  F04B49/065 ,  F04B53/08 ,  F04B53/143 ,  F04B9/042 ,  F16K15/183 ,  G01F1/34 ,  G01N1/40 ,  G01N30/28 ,  G21F5/015 ,  F04B2201/0209 ,  F04B2205/03 ,  F04B2205/05 ,  F04B2205/09 ,  F05B2230/41 ,  F05C2201/0493 ,  F05C2253/12 ,  G01N2001/4033 ,  G01N2001/4061 ,  G01N2030/6013 ,  G01N2035/0441 ,  G01N30/02

摘要： In a piston pump for pumping liquid carbon dioxide at a temperature below 30 degrees Fahrenheit and pressures at least as high as 7500 psi, the volume leaving the pump is determined by measuring only pressure or other parameter related to flow and movement of the plunger. The position of the piston is measured and the resulting displacement is integrated to determine volume of fluid pumped.

摘要翻译： 在用于在低于30华氏度的温度下泵送液体二氧化碳和至少高达7500psi的压力的活塞泵中，通过仅测量与柱塞的流动和运动相关的压力或其它参数来确定离开泵的体积。 测量活塞的位置，并将所得到的位移整合在一起，以确定泵送的流体体积。