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公开(公告)号：US08309796B2
公开(公告)日：2012-11-13
申请号：US13170011
申请日：2011-06-27
Applicant: Craig A. Weaver , Ross Zirkle , Daniel H. Doherty , James G. Metz
Inventor： Craig A. Weaver , Ross Zirkle , Daniel H. Doherty , James G. Metz
CPC classification number: C12P7/6427 , C12N9/93 , C12N15/52 , C12N15/8247 , C12N15/8273 , C12P7/62 , C12P7/625 , C12P7/6472
Abstract: Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.
Abstract translation: 公开了嵌合多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。
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公开(公告)号：US20120064573A1
公开(公告)日：2012-03-15
申请号：US13088830
申请日：2011-04-18
Applicant: George N. Cox , Daniel H. Doherty , Mary S. Rosendahl
Inventor： George N. Cox , Daniel H. Doherty , Mary S. Rosendahl
IPC: C12P21/00
CPC classification number: C12P21/02 , A61K38/27 , A61K47/60 , C07K1/1133 , C07K14/505 , C07K14/56 , C07K14/61 , C07K14/72 , C12P21/005
Abstract: The present invention relates to novel methods of making soluble proteins having free cysteines in which a host cell is exposed to a cysteine blocking agent. The soluble proteins produced by the methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form pegylated proteins.
Abstract translation: 本发明涉及制备具有游离半胱氨酸的可溶性蛋白质的新方法，其中宿主细胞暴露于半胱氨酸阻断剂。 然后可以对通过该方法产生的可溶性蛋白质进行修饰以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化的蛋白质。
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3.发明申请公开(公告)号：US20090269804A1
公开(公告)日：2009-10-29
申请号：US12001639
申请日：2007-12-11
Applicant: Mary S. Rosendahl , George N. Cox , Daniel H. Doherty
Inventor： Mary S. Rosendahl , George N. Cox , Daniel H. Doherty
CPC classification number: C07K14/52 , A61K47/60 , C07K1/1133 , C07K14/505 , C07K14/515 , C07K14/535 , C07K14/56 , C07K14/61 , C07K14/78
Abstract: The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.
Abstract translation: 本发明涉及制备和重折叠具有游离半胱氨酸的不溶性或聚集蛋白质的新方法，其中表达蛋白质的宿主细胞暴露于半胱氨酸阻断剂。 然后可以修饰通过新方法产生的可溶的，重折叠的蛋白质以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化蛋白质。
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公开(公告)号：US07153943B2
公开(公告)日：2006-12-26
申请号：US10298148
申请日：2002-11-15
Applicant: George N. Cox, III , Daniel H. Doherty
Inventor： George N. Cox, III , Daniel H. Doherty
IPC: C07K14/435 , C07K14/52 , C07K14/53 , C07K14/535 , A61K38/18 , A61K38/22 , A61K45/00 , C12N15/09 , C12N15/16
CPC classification number: A61K38/193 , A61K47/60 , C07K14/475 , C07K14/505 , C07K14/52 , C07K14/535 , C07K14/5431 , C07K14/61
Abstract: The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby. Also disclosed are therapeutic methods for using the cysteine variants of the invention.
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5.发明授权公开(公告)号：US07049406B2
公开(公告)日：2006-05-23
申请号：US10107871
申请日：2002-03-27
Applicant: Michael J Weickert , Christopher B Glascock , Antony J Mathews , Douglas D Lemon , Daniel H Doherty , John S Olson
Inventor： Michael J Weickert , Christopher B Glascock , Antony J Mathews , Douglas D Lemon , Daniel H Doherty , John S Olson
IPC: C07K14/00
CPC classification number: C07K14/805 , A61K38/00
Abstract: The invention relates to novel recombinant hemoglobins having reduced nitric oxide scavenging and/or increased high soluble expression. The invention further relates to methods of increasing the soluble expression of recombinant hemoglobin by adding exogenous hemin in molar excess of the heme binding sites of recombinant hemoglobin.
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Patent Agency Ranking
公开(公告)号：US20130217629A1
公开(公告)日：2013-08-22
申请号：US13616608
申请日：2012-09-14
Applicant: George N. Cox , Daniel H. Doherty , Mary S. Rosendahl
Inventor： George N. Cox , Daniel H. Doherty , Mary S. Rosendahl
IPC: A61K38/27
CPC classification number: C12P21/02 , A61K38/27 , A61K47/60 , C07K1/1133 , C07K14/505 , C07K14/56 , C07K14/61 , C07K14/72 , C12P21/005
Abstract: The present invention relates to novel methods of making soluble proteins having free cysteines in which a host cell is exposed to a cysteine blocking agent. The soluble proteins produced by the methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form pegylated proteins.
Abstract translation: 本发明涉及制备具有游离半胱氨酸的可溶性蛋白质的新方法，其中宿主细胞暴露于半胱氨酸阻断剂。 然后可以对通过该方法产生的可溶性蛋白质进行修饰以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化的蛋白质。
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公开(公告)号：US08148500B2
公开(公告)日：2012-04-03
申请号：US12886876
申请日：2010-09-21
Applicant: George N. Cox, III , Daniel H. Doherty
Inventor： George N. Cox, III , Daniel H. Doherty
CPC classification number: A61K38/193 , A61K47/60 , C07K14/475 , C07K14/505 , C07K14/52 , C07K14/535 , C07K14/5431 , C07K14/61
Abstract: The growth hormone supergene family comprises greater than 20 structurally related cytokines and growth factors. A general method is provided for creating site-specific, biologically active conjugates of these proteins. The method involves adding cysteine residues to non-essential regions of the proteins or substituting cysteine residues for non-essential amino acids in the proteins using site-directed mutagenesis and then covalently coupling a cysteine-reactive polymer or other type of cysteine-reactive moiety to the proteins via the added cysteine residue. Disclosed herein are preferred sites for adding cysteine residues or introducing cysteine substitutions into the proteins, and the proteins and protein derivatives produced thereby. Also disclosed are therapeutic methods for using the cysteine variants of the invention.
Abstract translation: 生长激素超基因家族包含大于20个结构相关的细胞因子和生长因子。 提供了一种用于产生这些蛋白质的位点特异性，生物活性缀合物的通用方法。 该方法包括将半胱氨酸残基添加到蛋白质的非必需区域中，或者使用定点突变将蛋白质中的非必需氨基酸取代半胱氨酸残基，然后将半胱氨酸反应性聚合物或其它类型的半胱氨酸反应性部分共价偶联到 通过添加的半胱氨酸残基的蛋白质。 本文公开了用于添加半胱氨酸残基或将半胱氨酸取代引入蛋白质的优选位点，以及由此产生的蛋白质和蛋白质衍生物。 还公开了使用本发明的半胱氨酸变体的治疗方法。
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公开(公告)号：US08003772B2
公开(公告)日：2011-08-23
申请号：US11749686
申请日：2007-05-16
Applicant: Craig A. Weaver , Ross Zirkle , Daniel H. Doherty , James G. Metz
Inventor： Craig A. Weaver , Ross Zirkle , Daniel H. Doherty , James G. Metz
CPC classification number: C12P7/6427 , C12N9/93 , C12N15/52 , C12N15/8247 , C12N15/8273 , C12P7/62 , C12P7/625 , C12P7/6472
Abstract: Disclosed are chimeric polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) proteins and chimeric PUFA PKS systems, including chimeric PUFA PKS proteins and systems derived from Schizochytrium and Thraustochytrium. Disclosed are nucleic acids and proteins encoding such chimeric PUFA PKS proteins and systems, genetically modified organisms comprising such chimeric PUFA PKS proteins and systems, and methods of making and using such chimeric PUFA PKS proteins and systems.
Abstract translation: 公开了嵌合多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）蛋白和嵌合PUFA PKS系统，包括嵌合PUFA PKS蛋白和源自裂殖壶菌和破囊壶菌的系统。 公开了编码这种嵌合PUFA PKS蛋白质和系统的核酸和蛋白质，包含这种嵌合PUFA PKS蛋白质和系统的遗传修饰的生物体，以及制备和使用这种嵌合PUFA PKS蛋白质和系统的方法。
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9.发明申请公开(公告)号：US20110189124A1
公开(公告)日：2011-08-04
申请号：US12893764
申请日：2010-09-29
Applicant: Mary S. Rosendahl , George N. Cox , Daniel H. Doherty
Inventor： Mary S. Rosendahl , George N. Cox , Daniel H. Doherty
CPC classification number: C07K14/52 , A61K47/60 , C07K1/1133 , C07K14/505 , C07K14/515 , C07K14/535 , C07K14/56 , C07K14/61 , C07K14/78
Abstract: The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins.
Abstract translation: 本发明涉及制备和重折叠具有游离半胱氨酸的不溶性或聚集蛋白质的新方法，其中表达蛋白质的宿主细胞暴露于半胱氨酸阻断剂。 然后可以修饰通过新方法产生的可溶的，重折叠的蛋白质以增加它们的有效性。 这样的修饰包括连接PEG部分以形成聚乙二醇化蛋白质。
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公开(公告)号：US20100285014A1
公开(公告)日：2010-11-11
申请号：US12777074
申请日：2010-05-10
Applicant: George N. Cox, III , Daniel H. Doherty
Inventor： George N. Cox, III , Daniel H. Doherty
CPC classification number: C07K14/61 , A61K38/00 , C07K14/475 , C07K14/505 , C07K14/521 , C07K14/524 , C07K14/535 , C07K14/5431 , C07K14/56 , C07K14/565 , C07K2319/00 , C07K2319/30
Abstract: The present invention relates to novel methods for making fusion proteins comprising a cytokine or growth factor fused to an immunoglobulin domain. The growth factor/cytokine can be fused directly to an immunoglobulin domain or through a peptide linker. The purified growth factor/cytokine-IgG fusion proteins produced by the novel methods are biologically active and can be used to treat diseases for which the non-fused growth factor/cytokine are useful.
Abstract translation: 本发明涉及制备包含与免疫球蛋白结构域融合的细胞因子或生长因子的融合蛋白的新方法。 生长因子/细胞因子可直接融合到免疫球蛋白结构域或通过肽接头。 通过新方法产生的纯化的生长因子/细胞因子-IgG融合蛋白具有生物活性，可用于治疗非融合生长因子/细胞因子有用的疾病。
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