公开(公告)号：US20230242856A1

公开(公告)日：2023-08-03

申请号：US18131620

申请日：2023-04-06

Applicant: David J. Beebe ,  Layla J. Barkal ,  Jason Yu

Inventor： David J. Beebe ,  Layla J. Barkal ,  Jason Yu

IPC: C12M3/06 ,  C12M1/32 ,  C12Q1/18

CPC classification number: C12M23/16 ,  C12M23/12 ,  C12Q1/18

Abstract: A platform and method for testing antibiotic sensitivity of a polymicrobial infection is provided. The platform includes a body defining a plurality of sets of chambers and a plurality of wells. Each set of chambers has a plurality of chambers adapted for culturing microbes of the polymicrobial infection therein. Each well is associated with a corresponding set of chambers and has an input in fluidic communication with the outlets of the plurality of chambers in the corresponding set of chambers. Selected antibiotic therapies may be received in the wells which fluidically connect the plurality of chambers in a corresponding set of chambers such that microbes cultured in the plurality of chambers in the corresponding set of chambers are in soluble factor contact.

公开(公告)号：US11430279B2

公开(公告)日：2022-08-30

申请号：US13467599

申请日：2012-05-09

Applicant: David J. Beebe ,  Erwin Berthler ,  Peter Cavnar ,  David John Guckenberger

Inventor： David J. Beebe ,  Erwin Berthler ,  Peter Cavnar ,  David John Guckenberger

IPC: B01L3/00 ,  G07C9/32 ,  B01L3/02 ,  G07C9/38

Abstract: A microfluidic platform and method are provided. The microfluidic platform includes a base having an outer surface and a plurality of wells formed in the outer surface thereof for receiving fluid therein. The plurality of wells are in fluid communication with each other. A lid includes a plurality of channels having corresponding inputs and outputs. The lid is moveable between a first position wherein the lid is disengaged from the base and a second position wherein the inputs of each channel communicate with corresponding wells in the base. The fluid in each well is drawn into corresponding channels through the inputs thereof by capillary action.

公开(公告)号：US08728410B2

公开(公告)日：2014-05-20

申请号：US13033351

申请日：2011-02-23

Applicant: David J. Beebe ,  Scott M. Berry ,  Richard Burgess ,  Lindsay Strotman

Inventor： David J. Beebe ,  Scott M. Berry ,  Richard Burgess ,  Lindsay Strotman

IPC: B03C1/02

CPC classification number: B01D21/0009 ,  B01D2221/10 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  C07K1/145 ,  C07K1/22 ,  C12N15/1006 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone.

Abstract translation: 提供了一种用于促进从生物样品中提取级分的装置和方法。 生物样品包括非期望的材料和分数结合的固相底物。 该装置包括用于在其中接收生物样本的输入区和用于在其中接收隔离缓冲器的相门区。 输出区在其中接收试剂。 力可在邻近输入区的第一位置与邻近输出区的第二位置之间移动。 力从输入区域通过相位闸区促进分数结合的固相基板进入输出区域。

公开(公告)号：US20140065654A1

公开(公告)日：2014-03-06

申请号：US13604192

申请日：2012-09-05

Applicant: David J. Beebe ,  Scott M. Berry ,  David J. Guckenberger ,  Ben P. Casavant

Inventor： David J. Beebe ,  Scott M. Berry ,  David J. Guckenberger ,  Ben P. Casavant

IPC: G01N1/34 ,  C12Q1/02

CPC classification number: G01N1/34 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/087 ,  B01L2400/043 ,  G01N1/4077 ,  G01N35/0098

Abstract: A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a second zone for receiving an isolation fluid therein. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone and a second position adjacent the isolation zone. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the isolation zone in response to the force moving from the first position to the second position.

Abstract translation: 提供了用于分离生物样品中的级分的装置和方法。 该馏分与固相底物结合以限定分馏结合的固相底物。 该装置包括用于在其中接收生物样品的输入区和用于在其中接收隔离流体的第二区。 提供通常垂直于重力的力。 该力可在邻近输入区的第一位置与邻近隔离区的第二位置之间移动。 该力捕获分馏结合的固相底物，并且部分结合的固相基板响应于从第一位置移动到第二位置的力而从输入区移动到隔离区。

公开(公告)号：US08628517B2

公开(公告)日：2014-01-14

申请号：US12821743

申请日：2010-06-23

Applicant: David J. Beebe ,  Michael J. MacDonald ,  David T. Eddington ,  Glennys A. Mensing

Inventor： David J. Beebe ,  Michael J. MacDonald ,  David T. Eddington ,  Glennys A. Mensing

IPC: A61K9/22

CPC classification number: A61M5/141 ,  A61M5/14248 ,  A61M5/14586 ,  A61M5/16881 ,  A61M2005/1726

Abstract: A microfluidic device is provided for delivering a drug to an individual. The microfluidic device includes a body that defines a reservoir for receiving the drug therein. A valve interconnects the reservoir to an output needle that is insertable into the skin of an individual. A pressure source urges the drug from the reservoir toward the needle. The valve is movable between a closed position preventing the flow of the drug from the reservoir to the output needle and an open position allowing for the flow of the drug from the reservoir to the output needle in response to a predetermined condition in the physiological fluids of the individual.

Abstract translation: 提供了用于将药物递送给个体的微流体装置。 微流体装置包括限定用于在其中接收药物的储存器的主体。 阀将储存器互连到可插入个人皮肤的输出针。 压力源将药物从储存器推向针。 所述阀可在关闭位置之间移动，所述关闭位置防止药物从所述贮存器流到所述输出针，以及打开位置，以允许所述药物从所述储存器流动到所述输出针，以响应于所述生理流体中的预定条件 个人

公开(公告)号：US20110213133A1

公开(公告)日：2011-09-01

申请号：US12713950

申请日：2010-02-26

Applicant: David J. Beebe ,  Scott M. Barry

Inventor： David J. Beebe ,  Scott M. Barry

IPC: C07K1/14 ,  C07H21/00 ,  G01N1/34

CPC classification number: C07K1/145 ,  C07K1/22

Abstract: A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone.

Abstract translation: 提供了一种用于促进从生物样品中提取级分的装置和方法。 生物样品包括非期望的材料和分数结合的固相底物。 该装置包括用于在其中接收生物样本的输入区和用于在其中接收隔离缓冲器的相门区。 输出区在其中接收试剂。 力可在邻近输入区的第一位置与邻近输出区的第二位置之间移动。 力从输入区域通过相位闸区促进分数结合的固相基板进入输出区域。

公开(公告)号：US20100030198A1

公开(公告)日：2010-02-04

申请号：US12184613

申请日：2008-08-01

Applicant: David J. Beebe ,  Benjamin J. Moga ,  Tony Escarcega ,  Kent Chase

Inventor： David J. Beebe ,  Benjamin J. Moga ,  Tony Escarcega ,  Kent Chase

IPC: A61K9/22

CPC classification number: A61M37/00 ,  A61M5/14248 ,  A61M5/145 ,  A61M5/148 ,  A61M2005/14252 ,  A61M2005/14513

Abstract: A drug delivery platform is provided for delivering a controlled infusion of a drug to an individual. The drug delivery platform includes a reservoir for receiving the drug therein and a pressure source engageable with the reservoir. The pressure source is movable between a first configuration and a second configuration wherein the pressure source exerts a pressure on the reservoir to urge the drug therefrom. An output conduit is provided for transmitting the drug into the individual. An actuation mechanism is operatively connected to the pressure source and the output conduit. The actuation mechanism is movable between a non-actuated position and an actuated position wherein pressure source moves from the first configuration to the second configuration and wherein the input of the output conduit communicates with the drug and the output end of the output conduit is receivable in the individual.

Abstract translation: 提供药物递送平台用于将受控的药物输注输送给个体。 药物递送平台包括用于在其中接收药物的储存器和可与贮存器接合的压力源。 压力源可在第一配置和第二配置之间移动，其中压力源对储存器施加压力以从其中推动药物。 提供用于将药物传输到个体中的输出导管。 致动机构可操作地连接到压力源和输出导管。 致动机构可在非致动位置和致动位置之间移动，其中压力源从第一构型移动到第二构型，并且其中输出导管的输入与药物连通，输出管道的输出端可接收在 个人

公开(公告)号：US07554743B2

公开(公告)日：2009-06-30

申请号：US11442927

申请日：2006-05-30

Applicant: Hongrui Jiang ,  Abhishek K. Agarwal ,  Liang Dong ,  David J. Beebe

Inventor： Hongrui Jiang ,  Abhishek K. Agarwal ,  Liang Dong ,  David J. Beebe

IPC: G02B1/06

CPC classification number: G02B3/14 ,  G02B3/0006

Abstract: A variable-focus lens assembly is provided. The lens assembly includes a microfluidic device that defines a chamber for receiving a fluid therein. A slip having an aperture therethrough is disposed in the chamber. A first fluid is disposed on the first side of the slip and a second fluid is disposed on the second side of the slip. A lens is formed from the interface of the first and second fluids. The outer periphery is pinned to the slip about the aperture. A turning structure fabricated from a hydrogel material engages the slip and tunes the focal length of the lens in response to a predetermined stimulus.

Abstract translation: 提供了可变焦距透镜组件。 透镜组件包括限定用于在其中接收流体的室的微流体装置。 具有通孔的滑动件设置在腔室中。 第一流体设置在滑动件的第一侧上，并且第二流体设置在滑动件的第二侧上。 透镜由第一和第二流体的界面形成。 外周围被钉住在孔周围的滑块上。 由水凝胶材料制成的转动结构与滑动接合并响应于预定的刺激调谐透镜的焦距。

公开(公告)号：US07553132B2

公开(公告)日：2009-06-30

申请号：US11133901

申请日：2005-05-20

Applicant: Hongrui Jiang ,  David J. Beebe ,  Abhishek K. Agarwal ,  Francisco J. Atencia-Fernandez

Inventor： Hongrui Jiang ,  David J. Beebe ,  Abhishek K. Agarwal ,  Francisco J. Atencia-Fernandez

IPC: F04B49/00 ,  C12M1/38 ,  F01D15/12 ,  F16D27/00

CPC classification number: B01F13/0059 ,  B01F13/0818 ,  F04B19/006

Abstract: A micro device is provided that includes a body defining a chamber for receiving fluid. A rotational element is disposed in the chamber for acting on the fluid. The rotational element is rotatable about an axis in response to a rotating magnetic field. The micro device further includes a clutch mechanism having a first disengaged configuration and a second engaged configuration wherein the clutch mechanism engages the rotational element and prevents rotation of the same.

Abstract translation: 提供了一种微型装置，其包括限定用于接收流体的室的主体。 旋转元件设置在腔室中用于作用在流体上。 旋转元件可响应于旋转磁场围绕轴线旋转。 微型装置还包括离合器机构，其具有第一分离构造和第二接合构造，其中离合器机构接合旋转元件并防止其旋转。

公开(公告)号：US07470403B2

公开(公告)日：2008-12-30

申请号：US11411671

申请日：2006-04-26

Applicant: David J. Beebe ,  Vinay V. Abhyankar

Inventor： David J. Beebe ,  Vinay V. Abhyankar

IPC: B01L3/02

CPC classification number: B01F13/0059 ,  B01F13/0093 ,  B01F15/0404 ,  B01L3/5027

Abstract: An apparatus and method of using the same are provided for generating a gradient of particles within a microfluidic device. The microfluidic device includes a channel having an input and an output. The channel is filled with a predetermined fluid. Thereafter, particles from a source pass through a porous membrane into the input of the channel. A second membrane is provided adjacent the output of the channel to minimize convection therein. A sink communicates with the output of the channel. The source/sink combination creates a pseudo-steady state in the channel wherein the concentration of particles at a point does not vary dramatically with time.

Abstract translation: 提供了一种使用其的装置和方法，用于在微流体装置内产生颗粒的梯度。 微流体装置包括具有输入和输出的通道。 通道填充有预定流体。 此后，来自源的颗粒通过多孔膜进入通道的输入。 在通道的输出附近设置第二膜以最小化其中的对流。 接收器与信道的输出通信。 源/汇组合在通道中产生伪稳态，其中点处的粒子浓度随时间而不会显着变化。