公开(公告)号：US07094604B2

公开(公告)日：2006-08-22

申请号：US10798192

申请日：2004-03-11

申请人： Richard O. Snyder ,  Sergei Zolotukhin ,  Yoshihisa Sakai ,  Barry J. Byrne ,  Mark R. Potter ,  Irine Zolotukhin ,  Scott Loiler ,  Vince A. Chiodo ,  Nicholas Muzyczka ,  William W. Hauswirth ,  Terence R. Flotte ,  Corinna Burger ,  Edgardo Rodriguez ,  Kevin R. Nash ,  Thomas J. Fraites

发明人： Richard O. Snyder ,  Sergei Zolotukhin ,  Yoshihisa Sakai ,  Barry J. Byrne ,  Mark R. Potter ,  Irine Zolotukhin ,  Scott Loiler ,  Vince A. Chiodo ,  Nicholas Muzyczka ,  William W. Hauswirth ,  Terence R. Flotte ,  Corinna Burger ,  Edgardo Rodriguez ,  Kevin R. Nash ,  Thomas J. Fraites

IPC分类号： C12N5/10 ,  C12N15/864 ,  C12N15/35

CPC分类号： C12N15/86 ,  C12N2710/10322 ,  C12N2750/14143 ,  C12N2840/20

摘要： Vectors that encode Adeno-Associated Virus (AAV) Rep and Cap proteins of different serotypes and Adenovirus transcription products that provide helper functions were used to produce pseudotyped recombinant AAV (rAAV) virions. Purification methods generated pseudotyped rAAV virion stocks that were 99% pure with titers of 1×1012–1×1013 vector genomes/ml.

摘要翻译： 使用编码提供辅助功能的不同血清型和腺病毒转录产物的腺相关病毒（AAV）Rep和Cap蛋白的载体产生假型重组AAV（rAAV）病毒粒子。 纯化方法产生99％纯度的假型分离的rAAV病毒颗粒，其滴度为1×10 12×10 13个载体基因组/ ml。

公开(公告)号：US6083562A

公开(公告)日：2000-07-04

申请号：US338022

申请日：1999-06-22

申请人： Edgardo Rodriguez ,  John W. Lindsay ,  William E. Streetman

发明人： Edgardo Rodriguez ,  John W. Lindsay ,  William E. Streetman

IPC分类号： D01F1/09 ,  D01F8/02 ,  D01F8/04 ,  D01F8/08 ,  D01F8/12 ,  D01F8/14 ,  B05D5/12

CPC分类号： D01F6/44 ,  D01D5/06 ,  D01F1/09 ,  D01F6/80 ,  D01F8/02 ,  D01F8/08 ,  D01F8/12 ,  D01F8/14 ,  Y10T428/2918 ,  Y10T428/292 ,  Y10T428/2924 ,  Y10T428/2929 ,  Y10T428/2933 ,  Y10T428/2967 ,  Y10T428/2969 ,  Y10T428/2971

摘要： A process and materials made by the process which includes a bicomponent fiber, made of a nonconductive first component, including a first fiber-forming polymer selected from the group consisting of polyethylene terephthalate, nylon 6, nylon 6,6, cellulose, polypropylene cellulose acetate, polyacrylonitrile and copolymers of polyacrylonitrile; a conductive second component, including carbon particles and a second fiber-forming polymer selected from the group consisting of polyethylene terephthalate, nylon 6, nylon 6,6, cellulose, polypropylene cellulose acetate, polyacrylonitrile and copolymers of polyacrylonitrile; and a conductive third component, including a polymer selected from the group consisting of polypyrrole and polyaniline, said polymer formed in situ and being interspersed among at least a portion of the carbon particles of the second component.

摘要翻译： 该方法制备的方法和材料包括由非导电的第一组分制成的双组分纤维，包括选自聚对苯二甲酸乙二醇酯，尼龙6，尼龙6,6，纤维素，聚丙烯纤维素乙酸纤维素的第一纤维形成聚合物 ，聚丙烯腈和聚丙烯腈的共聚物; 导电第二组分，包括碳颗粒和选自聚对苯二甲酸乙二醇酯，尼龙6，尼龙6,6，纤维素，聚丙烯纤维素乙酸酯，聚丙烯腈和聚丙烯腈共聚物的第二纤维形成聚合物; 和导电性第三组分，包括选自聚吡咯和聚苯胺的聚合物，所述聚合物原位形成并且散布在第二组分的至少一部分碳颗粒中。

公开(公告)号：US5972499A

公开(公告)日：1999-10-26

申请号：US869081

申请日：1997-06-04

申请人： Edgardo Rodriguez ,  John W. Lindsay ,  William E. Streetman

发明人： Edgardo Rodriguez ,  John W. Lindsay ,  William E. Streetman

IPC分类号： D01F1/09 ,  D01F8/02 ,  D01F8/04 ,  D01F8/08 ,  D01F8/12 ,  D01F8/14 ,  B32B27/04 ,  B32B27/20 ,  D02G3/40

CPC分类号： D01F6/44 ,  D01D5/06 ,  D01F1/09 ,  D01F6/80 ,  D01F8/02 ,  D01F8/08 ,  D01F8/12 ,  D01F8/14 ,  Y10T428/2918 ,  Y10T428/292 ,  Y10T428/2924 ,  Y10T428/2929 ,  Y10T428/2933 ,  Y10T428/2967 ,  Y10T428/2969 ,  Y10T428/2971

摘要： A process and materials made by the process which includes a bicomponent fiber, made of a nonconductive first component, including a first fiber-forming polymer selected from the group consisting of polyethylene terephthalate, nylon 6, nylon 6,6, cellulose, polypropylene cellulose acetate, polyacrylonitrile and copolymers of polyacrylonitrile; a conductive second component, including carbon particles and a second fiber-forming polymer selected from the group consisting of polyethylene terephthalate, nylon 6, nylon 6,6, cellulose, polypropylene cellulose acetate, polyacrylonitrile and copolymers of polyacrylonitrile; and a conductive third component, including a polymer selected from the group consisting of polypyrrole and polyaniline, said polymer formed in situ and being interspersed among at least a portion of the carbon particles of the second component.

摘要翻译： 该方法制备的方法和材料包括由非导电的第一组分制成的双组分纤维，包括选自聚对苯二甲酸乙二醇酯，尼龙6，尼龙6,6，纤维素，聚丙烯纤维素乙酸纤维素的第一纤维形成聚合物 ，聚丙烯腈和聚丙烯腈的共聚物; 导电第二组分，包括碳颗粒和选自聚对苯二甲酸乙二醇酯，尼龙6，尼龙6,6，纤维素，聚丙烯纤维素乙酸酯，聚丙烯腈和聚丙烯腈共聚物的第二纤维形成聚合物; 和导电性第三组分，包括选自聚吡咯和聚苯胺的聚合物，所述聚合物原位形成并且散布在第二组分的至少一部分碳颗粒中。

公开(公告)号：US20070015238A1

公开(公告)日：2007-01-18

申请号：US10456423

申请日：2003-06-05

申请人： Richard Snyder ,  Sergie Zolotukhin ,  Yoshihisa Sakai ,  Barry Byrne ,  Mark Potter ,  Irine Zolotukhin ,  Scott Loiler ,  Vince Chiodo ,  Nicholas Muzyczka ,  William Hauswirth ,  Terence Flotte ,  Corinna Burger ,  Edgardo Rodriguez

发明人： Richard Snyder ,  Sergie Zolotukhin ,  Yoshihisa Sakai ,  Barry Byrne ,  Mark Potter ,  Irine Zolotukhin ,  Scott Loiler ,  Vince Chiodo ,  Nicholas Muzyczka ,  William Hauswirth ,  Terence Flotte ,  Corinna Burger ,  Edgardo Rodriguez

IPC分类号： C12P21/06 ,  C12N7/00 ,  C12N15/861 ,  C12N5/06

CPC分类号： C12N15/86 ,  C12N2710/10322 ,  C12N2750/14143 ,  C12N2840/20

摘要： Vectors that encode Adeno-Associated Virus (AAV) Rep and Cap proteins of different serotypes and Adenovirus transcription products that provide helper functions were used to produce pseudotyped recombinant AAV (rAAV) virions. Purification methods generated pseudotyped rAAV virion stocks that were 99% pure with titers of 1×1012−1×1013 vector genomes/ml.

摘要翻译： 使用编码提供辅助功能的不同血清型和腺病毒转录产物的腺相关病毒（AAV）Rep和Cap蛋白的载体产生假型重组AAV（rAAV）病毒粒子。 纯化方法产生99％纯度的假型分离的rAAV病毒颗粒，其滴度为1×10 12×10 13个载体基因组/ ml。