公开(公告)号：US06736978B1

公开(公告)日：2004-05-18

申请号：US10017467

申请日：2001-12-13

申请人： Marc D. Porter ,  Jing Ni ,  G. Brent Dawson ,  Ruth Shinar ,  Robert J. Lipert ,  Michael C. Granger ,  Mark Tondra

发明人： Marc D. Porter ,  Jing Ni ,  G. Brent Dawson ,  Ruth Shinar ,  Robert J. Lipert ,  Michael C. Granger ,  Mark Tondra

IPC分类号： G01N33553

CPC分类号： G01F1/708 ,  G01N33/54333 ,  Y10S436/806

摘要： Method and apparatus for manipulating and monitoring analyte flowing in fluid streams. A giant magnetoresistive sensor has an array of sensing elements that produce electrical output signals which vary in dependence on changes in the magnetic field proximate the sensing elements. The analyte is included in a stream, such that the stream has a magnetic property which is dependent on the concentration and distribution on the analyte therein. The stream is flowed past the giant magnetoresistive sensor and in sufficiently close proximity to cause the magnetic properties of the stream to produce electrical output signals. The electrical output signals are monitored as an indicator of analyte concentration or distribution in the stream flowing past the GMR sensor. Changes in the magnetic field produced by the background stream are introduced by analyte molecules, whose presence in the flow past the GMR will effect the output reading.

摘要翻译： 用于操纵和监测在流体流中流动的分析物的方法和装置。 巨磁阻传感器具有产生电输出信号的感测元件的阵列，该电输出信号根据感测元件附近的磁场的变化而变化。 分析物被包含在流中，使得流具有取决于其上的分析物的浓度和分布的磁性。 流流过巨磁电阻传感​​器并且足够靠近以使流的磁特性产生电输出信号。 监测电输出信号作为流过GMR传感器的流中分析物浓度或分布的指标。 由背景流产生的磁场的变化由分析物分子引入，其在流过GMR的流中的存在将影响输出读数。

公开(公告)号：US07829348B2

公开(公告)日：2010-11-09

申请号：US10931142

申请日：2004-08-31

申请人： Marc D. Porter ,  Jing Ni ,  Robert J. Lipert ,  G. Brent Dawson

发明人： Marc D. Porter ,  Jing Ni ,  Robert J. Lipert ,  G. Brent Dawson

IPC分类号： G01N33/553

CPC分类号： C07H21/02 ,  G01N21/658 ,  G01N33/532 ,  G01N33/54373 ,  G01N33/553 ,  G01N33/58 ,  G01N33/587

摘要： The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).

摘要翻译： 本发明提供用于生物和其它应用的新类型的拉曼活性试剂，以及用于其使用和制造的方法和试剂盒。 每种试剂包括拉曼活性报道分子，结合分子和能够引起表面增强拉曼散射（SERS）的表面增强颗粒。 拉曼活性报道分子和结合分子固定在颗粒上，以产生强的SERS信号并提供生物功能，即抗原或药物识别。 拉曼活性试剂可以作为荧光标记试剂的替代品，具有检测的优点，包括信号稳定性，灵敏度和同时检测几种生物材料的能力。 拉曼活性试剂也具有广泛的应用，特别是在临床领域（例如免疫测定，成像和药物筛选）中。

公开(公告)号：US20110070662A1

公开(公告)日：2011-03-24

申请号：US12894810

申请日：2010-09-30

申请人： Marc D. Porter ,  Jing Ni ,  Robert J. Lipert ,  G. Brent Dawson

发明人： Marc D. Porter ,  Jing Ni ,  Robert J. Lipert ,  G. Brent Dawson

IPC分类号： G01N33/53 ,  G01N21/00

CPC分类号： C07H21/02 ,  G01N21/658 ,  G01N33/532 ,  G01N33/54373 ,  G01N33/553 ,  G01N33/58 ,  G01N33/587

摘要： The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).

摘要翻译： 本发明提供用于生物和其它应用的新类型的拉曼活性试剂，以及用于其使用和制造的方法和试剂盒。 每种试剂包括拉曼活性报道分子，结合分子和能够引起表面增强拉曼散射（SERS）的表面增强颗粒。 拉曼活性报道分子和结合分子固定在颗粒上，以产生强的SERS信号并提供生物功能，即抗原或药物识别。 拉曼活性试剂可以作为荧光标记试剂的替代品，具有检测的优点，包括信号稳定性，灵敏度和同时检测几种生物材料的能力。 拉曼活性试剂也具有广泛的应用，特别是在临床领域（例如免疫测定，成像和药物筛选）中。

公开(公告)号：US20110070661A1

公开(公告)日：2011-03-24

申请号：US12894693

申请日：2010-09-30

申请人： MARC D. PORTER ,  JING NI ,  ROBERT J. LIPERT ,  G. BRENT DAWSON

发明人： MARC D. PORTER ,  JING NI ,  ROBERT J. LIPERT ,  G. BRENT DAWSON

IPC分类号： G01N33/53 ,  G01N21/00

CPC分类号： G01N33/553 ,  G01N21/658 ,  G01N33/54373 ,  G01N33/587 ,  Y10T436/24

摘要： The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).

公开(公告)号：US07824926B1

公开(公告)日：2010-11-02

申请号：US09961628

申请日：2001-09-24

申请人： Marc D. Porter ,  Jing Ni ,  Robert J. Lipert ,  G. Brent Dawson

发明人： Marc D. Porter ,  Jing Ni ,  Robert J. Lipert ,  G. Brent Dawson

IPC分类号： G01N33/553

CPC分类号： G01N33/553 ,  G01N21/658 ,  G01N33/54373 ,  G01N33/587 ,  Y10T436/24

摘要： The present invention provides a new class of Raman-active reagents for use in biological and other applications, as well as methods and kits for their use and manufacture. Each reagent includes a Raman-active reporter molecule, a binding molecule, and a surface enhancing particle capable of causing surface enhanced Raman scattering (SERS). The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening).

公开(公告)号：US07179383B1

公开(公告)日：2007-02-20

申请号：US10793058

申请日：2004-03-04

申请人： Marc D Porter ,  Jing Ni ,  G Brent Dawson ,  Ruth Shinar ,  Robert J Lipert ,  Michael C Granger ,  Mark Tondra

发明人： Marc D Porter ,  Jing Ni ,  G Brent Dawson ,  Ruth Shinar ,  Robert J Lipert ,  Michael C Granger ,  Mark Tondra

IPC分类号： B03C1/00 ,  G01N33/553

CPC分类号： G01F1/708 ,  G01N33/54333 ,  Y10S436/806

摘要： Method and apparatus for manipulating and monitoring analyte flowing in fluid streams. A giant magnetoresistive sensor has an array of sensing elements that produce electrical output signals which vary in dependence on changes in the magnetic field proximate the sensing elements. The analyte is included in a stream, such that the stream has a magnetic property which is dependent on the concentration and distribution on the analyte therein. The stream is flowed past the giant magnetoresistive sensor and in sufficiently close proximity to cause the magnetic properties of the stream to produce electrical output signals. The electrical output signals are monitored as an indicator of analyte concentration or distribution in the stream flowing past the GMR sensor. Changes in the magnetic field produced by the background stream are introduced by analyte molecules, whose presence in the flow past the GMR will effect the output reading.

摘要翻译： 用于操纵和监测在流体流中流动的分析物的方法和装置。 巨磁阻传感器具有产生电输出信号的感测元件的阵列，该电输出信号根据感测元件附近的磁场的变化而变化。 分析物被包含在流中，使得流具有取决于其上的分析物的浓度和分布的磁性。 流流过巨磁电阻传感​​器并且足够靠近以使流的磁特性产生电输出信号。 监测电输出信号作为流过GMR传感器的流中分析物浓度或分布的指标。 由背景流产生的磁场的变化由分析物分子引入，其在流过GMR的流中的存在将影响输出读数。