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1.发明授权
公开(公告)号：US5620850A
公开(公告)日：1997-04-15
申请号：US312388
申请日：1994-09-26
IPC分类号： C12Q1/68 , G01N33/543 , C07K2/00 , C07K5/00 , G01N33/53
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a species of a formula X--R--Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
摘要翻译： 适合用作生物传感器的制品包括作为自组装单层的一部分粘附到制品表面的式X-R-Ch的种类。 X是附着于表面的官能团，R是间隔基，Ch是螯合剂。 金属离子可以由螯合剂配位，并且靶向生物分子的聚氨基酸标记的生物结合配偶体与金属离子配位。 本发明的方法包括使制品与含有或怀疑含有靶生物分子的培养基接触并允许生物分子与结合配偶体生物结合。 该文章特别用作表面等离子体共振芯片。
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2.发明授权公开(公告)号：US06809196B2
公开(公告)日：2004-10-26
申请号：US09915187
申请日：2001-07-25
IPC分类号： C07H2104
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
摘要翻译： 适合用作生物传感器的制品包括作为自组装单层的一部分粘附到制品表面的式X-R-Ch分子。 X是附着于表面的官能团，R是间隔基，Ch是螯合剂。 金属离子可以由螯合剂配位，并且靶向生物分子的聚氨基酸标记的生物结合配偶体与金属离子配位。 本发明的方法包括使制品与含有或怀疑含有靶生物分子的培养基接触并允许生物分子与结合配偶体生物结合。 该文章特别用作表面等离子体共振芯片。
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公开(公告)号：US08722323B2
公开(公告)日：2014-05-13
申请号：US12917661
申请日：2010-11-02
申请人： Jacob N. Wohlstadter , James L. Wilbur , George B. Sigal , Mark T. Martin , Liang-Hong Guo , Alan Fischer , Jon Leland , Mark A. Billadeau , Larry R. Helms , Ramin Darvari
发明人： Jacob N. Wohlstadter , James L. Wilbur , George B. Sigal , Mark T. Martin , Liang-Hong Guo , Alan Fischer , Jon Leland , Mark A. Billadeau , Larry R. Helms , Ramin Darvari
IPC分类号： C12Q1/00
CPC分类号： G01N21/76 , B01L3/5027 , B01L3/502715 , B01L2300/0636 , B01L2300/0645 , B82Y15/00 , B82Y30/00 , C12Q1/6825 , G01N21/253 , G01N21/66 , G01N33/54313 , G01N33/5438 , G01N33/582 , G01N33/585 , Y10S435/808 , Y10S435/817 , Y10S436/805 , Y10S436/806 , Y10S436/807
摘要： Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
摘要翻译： 提供材料和方法用于生产用于诊断的图案化多阵列多特异性表面。 本发明提供了用于检测或测量感兴趣分析物的电化学发光方法。 它还提供用于ECL测定的新型电极。 提供材料和方法用于化学和/或物理控制导电域和试剂沉积，以便使用多重特定的测试程序。
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公开(公告)号：US20110124116A1
公开(公告)日：2011-05-26
申请号：US12917661
申请日：2010-11-02
申请人： Jacob N. WOHLSTADTER , James L. Wilbur , George B. Sigal , Mark T. Martin , Liang-Hong Guo , Alan Fischer , Jon Leland , Mark A. Billadeau , Larry R. Helms , Ramin Darvari
发明人： Jacob N. WOHLSTADTER , James L. Wilbur , George B. Sigal , Mark T. Martin , Liang-Hong Guo , Alan Fischer , Jon Leland , Mark A. Billadeau , Larry R. Helms , Ramin Darvari
IPC分类号： G01N21/76
CPC分类号： G01N21/76 , B01L3/5027 , B01L3/502715 , B01L2300/0636 , B01L2300/0645 , B82Y15/00 , B82Y30/00 , C12Q1/6825 , G01N21/253 , G01N21/66 , G01N33/54313 , G01N33/5438 , G01N33/582 , G01N33/585 , Y10S435/808 , Y10S435/817 , Y10S436/805 , Y10S436/806 , Y10S436/807
摘要： Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
摘要翻译： 提供材料和方法用于生产用于诊断的图案化多阵列多特异性表面。 本发明提供了用于检测或测量感兴趣分析物的电化学发光方法。 它还提供用于ECL测定的新型电极。 提供材料和方法用于化学和/或物理控制导电域和试剂沉积，以便使用多重特定的测试程序。
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5.发明申请公开(公告)号：US20100140086A1
公开(公告)日：2010-06-10
申请号：US12591528
申请日：2009-11-23
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
摘要翻译： 提出了一种用于进行电化学发光测定的方法和装置。 在本发明的示例性实施方案中，一种装置包括其上固定有第一测定配体的ECL非活性电极表面和其上固定有第二测定配体的ECL活性微粒。 在本发明的示例性实施方案中，方法包括（a）形成包含电化学发光标记和其上固定有第一测定配体的微粒的混合物，（b）在所述混合物存在下将电化学能施加到电极表面，以及 （c）测量发射的电化学发光，其中微粒是ECL-活性的，并且当在所述混合物的存在下施加电化学能时，电极表面是ECL-无活性的。
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公开(公告)号：US06472148B1
公开(公告)日：2002-10-29
申请号：US08786187
申请日：1997-01-21
IPC分类号： C12Q168
CPC分类号： C12Q1/6837 , B82Y15/00 , B82Y30/00 , B82Y40/00 , G01N33/54373 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
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公开(公告)号：US06322979B1
公开(公告)日：2001-11-27
申请号：US09296078
申请日：1999-04-21
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
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8.发明授权公开(公告)号：US06319670B1
公开(公告)日：2001-11-20
申请号：US08998137
申请日：1997-12-23
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
IPC分类号： C12Q168
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： The present invention relates to methods, reagents and compositions, for conducting electrochemiluminescence binding assays which improve one or more characteristics of the assay or the instruments used to conduct the assay. The method is achieved using microparticles that include electrically conductive material. The electrically conductive material has one or more copies of an assay ligand immobilized an its outer surface and a plurality of electrochemiluminescent moieties immobilized an its outer surface. The assay ligand may be linked to the electrochemiluminescent moiety.
摘要翻译： 本发明涉及用于进行电化学发光结合测定法的方法，试剂和组合物，其改进测定的一个或多个特征或用于进行测定的仪器。 该方法使用包括导电材料的微粒来实现。 导电材料具有固定其外表面的测定配体的一个或多个拷贝和固定其外表面的多个电化学发光部分。 测定配体可以连接到电化学发光部分。
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9.发明授权公开(公告)号：US06197515B1
公开(公告)日：2001-03-06
申请号：US09296111
申请日：1999-04-21
IPC分类号： C12Q168
CPC分类号： B82Y30/00 , B82Y15/00 , B82Y40/00 , C07H21/04 , C12Q1/6837 , G01N33/54353 , G01N33/54373 , G01N33/553 , G01N2610/00
摘要： An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
摘要翻译： 适合用作生物传感器的制品包括作为自组装单层的一部分粘附到制品表面的式X-R-Ch分子。 X是附着于表面的官能团，R是间隔基，Ch是螯合剂。 金属离子可以由螯合剂配位，并且靶向生物分子的聚氨基酸标记的生物结合配偶体与金属离子配位。 本发明的方法包括使制品与含有或怀疑含有靶生物分子的培养基接触并允许生物分子与结合配偶体生物结合。 该文章特别用作表面等离子体共振芯片。
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10.发明授权公开(公告)号：US09448235B2
公开(公告)日：2016-09-20
申请号：US12591528
申请日：2009-11-23
申请人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
发明人： George B. Sigal , Jacob N. Wohlstadter , Satyanarayana Gudibande , Mark T. Martin , James L. Wilbur
IPC分类号： G01N27/26 , G01N33/543 , B82Y30/00 , C07H21/00 , C12Q1/68 , G01N33/532 , G01N33/533 , G01N33/58
CPC分类号： G01N33/54346 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6816 , C12Q1/6825 , C12Q1/6834 , G01N33/532 , G01N33/533 , G01N33/54306 , G01N33/54366 , G01N33/5438 , G01N33/552 , G01N33/553 , G01N33/582 , G01N33/587 , G01N2458/30 , C12Q2563/149 , C12Q2563/113 , C12Q2537/125
摘要： A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
摘要翻译： 提出了一种用于进行电化学发光测定的方法和装置。 在本发明的示例性实施方案中，一种装置包括其上固定有第一测定配体的ECL非活性电极表面和其上固定有第二测定配体的ECL活性微粒。 在本发明的示例性实施方案中，方法包括（a）形成包含电化学发光标记和其上固定有第一测定配体的微粒的混合物，（b）在所述混合物存在下将电化学能施加到电极表面，以及 （c）测量发射的电化学发光，其中微粒是ECL-活性的，并且当在所述混合物的存在下施加电化学能时，电极表面是ECL-无活性的。
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