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公开(公告)号：US5702912A
公开(公告)日：1997-12-30
申请号：US564986
申请日：1995-11-30
CPC分类号： C12Q1/56 , G01N2333/815 , G01N2333/974 , G01N2400/40
摘要： The present invention relates to a method to determine the concentration of anticoagulants as a function of the inhibition of thrombin formation in an assay mixture. The assay mixture is composed of two reagents and the diluted plasma sample. The diluted plasma sample containing an anticoagulatory substance is mixed with a clotting factor reagent, which is a combination of an excess amount of purified coagulation factors of the endogenous system, phospholipids and a weak thrombin inhibitor, which can be potentiated by an anticoagulatory substance. Then a coagulation cascade activator and calcium chloride, an activator reagent, is added to the mixture and formed thrombin is measured after a chosen incubation time. A proportionality between the inhibition of the thrombin formation and the anticoagulatory active components allows for a determination of the concentration of anticoagulatory active components in the sample.
摘要翻译： 本发明涉及一种确定抗凝血剂浓度作为测定混合物中凝血酶形成抑制的函数的方法。 测定混合物由两种试剂和稀释的血浆样品组成。 含有抗凝物质的稀释血浆样品与凝血因子试剂混合，凝血因子试剂是过量的内源性系统的纯化凝血因子，磷脂和弱凝血酶抑制剂的组合，其可以被抗凝物质加强。 然后将凝血级联活化剂和氯化钙（活化剂）加入到混合物中，并在选择的孵育时间后测量形成的凝血酶。 抑制凝血酶形成与抗凝活性成分之间的比例允许测定样品中抗凝活性成分的浓度。
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公开(公告)号：US5231006A
公开(公告)日：1993-07-27
申请号：US582779
申请日：1990-09-14
申请人： Hans-Jurgen Kolde
发明人： Hans-Jurgen Kolde
CPC分类号： C12Q1/56 , C12Q1/37 , C12Q2337/12 , G01N2333/3153 , G01N2333/968 , G01N2333/972
摘要： A method for the determination of plasminogen in body fluids in the presence of streptokinase using a substrate for plasmin, the action of plasmin on which results in a cleavage product which can be measured optically, which method comprises essentially simultaneous addition of streptokinase and chromogenic or fluorogenic substrate for plasmin to the body fluid, and determination of the amount of the cleavage product formed in a defined time, or of the rate of formation of the cleavage product, and from this the concentration of plasminogen, is described. When the amount of streptokinase which is used is such that the plasminogen present is in molar excess, this method can be used to determine the rate of activation of plasminogen.
摘要翻译： 在使用纤溶酶底物的链激酶存在下测定体液中的纤溶酶原的方法，其中纤溶酶的作用导致可以光学测量的切割产物，该方法基本上同时加入链激酶和显色或荧光 描述了纤溶酶对体液的底物，以及在规定时间内形成的切割产物的量或切割产物的形成速率以及由此引起的纤溶酶原浓度的测定。 当使用的链激酶的量使得存在的纤溶酶原摩尔过量时，该方法可用于测定纤溶酶原的活化速率。
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3.发明授权Prothrombin time determining reagent and methods of using and preparing the same 失效凝血酶原时间测定试剂及其制备方法
公开(公告)号：US4784944A
公开(公告)日：1988-11-15
申请号：US720348
申请日：1985-04-05
申请人： Hans-Jurgen Kolde
发明人： Hans-Jurgen Kolde
CPC分类号： G01N33/86 , C12Q2337/12 , G01N2333/745 , G01N2333/974
摘要： A reagent for the photometric determination of the prothrombin time is described. The reagent contains a thromboplastin and a chromogenic substrate for thrombin, wherein this substrate is cleaved by the thromboplastin at only a low reaction rate. This reagent can be used for the determination of coagulation factors with the use of deficient plasma. Also disclosed is a method of preparing the reagent.
摘要翻译： 描述了用于光度测定凝血酶原时间的试剂。 该试剂含有凝血激酶和凝血酶的显色底物，其中该底物仅以低反应速率被凝血激酶切割。 该试剂可用于使用缺乏血浆的凝血因子的测定。 还公开了制备试剂的方法。
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公开(公告)号：US20110201025A1
公开(公告)日：2011-08-18
申请号：US13026474
申请日：2011-02-14
申请人： Hans-Jurgen Kolde , Ute Lange , Elke Bucha
发明人： Hans-Jurgen Kolde , Ute Lange , Elke Bucha
CPC分类号： G01N33/86 , C12N9/6429 , C12N9/647 , C12Y304/21005
摘要： The present invention is directed to a simple process for the modification of the specificity of a peptidase of the hemostatic system by coupling polymers to the peptidase causing it to lose its reactivity in the hemostatic system, but enabling it to continue to react with certain inhibitors, effectors and substrates. The invention is furthermore directed to processes for the detection or quantitative determination of inhibitors of the peptidase in bodily fluids or other samples as well as to processes for their neutralization and/or removal from liquids. Finally, the invention allows the use of a polymer-coupled peptidase as drug and furthermore provides a device which makes use of such a peptidase in the removal of peptidase inhibitors from samples or from the bloodstream of a patient.
摘要翻译： 本发明涉及通过将聚合物偶联至肽酶来改变止血系统肽酶的特异性的简单方法，导致其在止血系统中失去其反应性，但使其能够与某些抑制剂继续反应， 效应器和基板。 本发明还涉及用于检测或定量测定体液或其它样品中肽酶抑制剂的方法，以及其中和和/或从液体中除去的方法。 最后，本发明允许使用聚合物偶联的肽酶作为药物，并且还提供一种使用这种肽酶从样品或来自患者的血液中除去肽酶抑制剂的装置。
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公开(公告)号：US09329192B2
公开(公告)日：2016-05-03
申请号：US13026474
申请日：2011-02-14
申请人： Hans-Jurgen Kolde , Ute Lange , Elke Bucha
发明人： Hans-Jurgen Kolde , Ute Lange , Elke Bucha
CPC分类号： G01N33/86 , C12N9/6429 , C12N9/647 , C12Y304/21005
摘要： The present invention is directed to a simple process for the modification of the specificity of a peptidase of the hemostatic system by coupling polymers to the peptidase causing it to lose its reactivity in the hemostatic system, but enabling it to continue to react with certain inhibitors, effectors and substrates. The invention is furthermore directed to processes for the detection or quantitative determination of inhibitors of the peptidase in bodily fluids or other samples as well as to processes for their neutralization and/or removal from liquids. Finally, the invention allows the use of a polymer-coupled peptidase as drug and furthermore provides a device which makes use of such a peptidase in the removal of peptidase inhibitors from samples or from the bloodstream of a patient.
摘要翻译： 本发明涉及通过将聚合物偶联至肽酶来改变止血系统肽酶的特异性的简单方法，导致其在止血系统中失去其反应性，但使其能够与某些抑制剂继续反应， 效应器和基板。 本发明还涉及用于检测或定量测定体液或其它样品中肽酶抑制剂的方法，以及其中和和/或从液体中除去的方法。 最后，本发明允许使用聚合物偶联的肽酶作为药物，并且还提供一种使用这种肽酶从样品或来自患者的血液中除去肽酶抑制剂的装置。
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公开(公告)号：US4918001A
公开(公告)日：1990-04-17
申请号：US903458
申请日：1986-09-04
申请人： Hans-Jurgen Kolde
发明人： Hans-Jurgen Kolde
IPC分类号： C12N9/99 , C12Q1/37 , C12Q1/56 , G01N33/547
CPC分类号： C12Q1/56 , C12Q1/37 , C12Q2337/12 , G01N2333/8128 , G01N2333/96444 , G01N2333/974
摘要： A method for the determination of a protease inhibitor using a protease which can be inhibited by this inhibitor, and using a substrate for this protease, is described, in which initially a liquid containing the protease inhibitor and the substrate are mixed, then the protease is added, and the rate of hydrolysis of the substrate is determined.
摘要翻译： 描述了使用可以被该抑制剂抑制的蛋白酶和使用该蛋白酶的底物来测定蛋白酶抑制剂的方法，其中最初将含有蛋白酶抑制剂和底物的液体混合，然后将蛋白酶为 并测定底物的水解速率。
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