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    Complexes containing crosslinked avidin, analytical method with the use of crosslinked avidin and analytical reagents and kits
    1.
    发明授权
    Complexes containing crosslinked avidin, analytical method with the use of crosslinked avidin and analytical reagents and kits 有权
    含有交联抗生物素蛋白的配合物，使用交联抗生物素蛋白的分析方法和分析试剂和试剂盒

    公开(公告)号：US06787325B1

    公开(公告)日：2004-09-07

    申请号：US09582842

    申请日：2000-07-05

    申请人： Kazuyuki Sugiyama ,  Nobuhiro Hoshino ,  Hiroki Tatsumi ,  Satoshi Fukuda

    发明人： Kazuyuki Sugiyama ,  Nobuhiro Hoshino ,  Hiroki Tatsumi ,  Satoshi Fukuda

    IPC分类号： C12Q166

    CPC分类号： G01N33/53 ,  Y10S435/975

    摘要： Provided are novel complexes containing a crosslinked avidin, an analyzing method and analyzing reagents and kits whereby a compound to be analyzed can be quickly, conveniently and accurately analyzed while taking advantage of the avidin-biotin reaction. The complexes contain at least two homogeneous or heterogeneous biotin-introduced products and one crosslinked avidin sandwiched therebetween. In the analyzing method, the homogeneous or heterogeneous biotin-introduced products and the crosslinked avidin are used. The analyzing reagent contains the crosslinked avidin. The analyzing kit contains the crosslinked avidin and a biotinylating agent.

    摘要翻译： 提供了含有交联抗生物素蛋白的新型配合物，分析方法和分析试剂和试剂盒，由此可以快速，方便和准确地分析待分析的化合物，同时利用抗生物素蛋白 - 生物素反应。 复合物含有至少两种均相或不均匀的生物素引入产物和一个夹在其间的交联亲和素。 在分析方法中，使用均相或异质生物素引入的产物和交联的亲和素。 分析试剂含有交联的亲和素。 分析试剂盒含有交联抗生物素蛋白和生物素化剂。

    Pyruvate orthophosphate dikinase gene, recombinant DNA, and process for producing pyruvate orthophosphate dikinase
    2.
    发明授权
    Pyruvate orthophosphate dikinase gene, recombinant DNA, and process for producing pyruvate orthophosphate dikinase 失效
    丙酮酸正磷酸二激酶基因，重组DNA，以及产生丙酮酸正磷酸二激酶的方法

    公开(公告)号：US6054305A

    公开(公告)日：2000-04-25

    申请号：US941936

    申请日：1997-10-01

    申请人： Hiroki Tatsumi ,  Naoki Eisaki ,  Tatsuo Horiuchi ,  Ayumu Nagahara

    发明人： Hiroki Tatsumi ,  Naoki Eisaki ,  Tatsuo Horiuchi ,  Ayumu Nagahara

    IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12Q1/48 ,  C12R1/19 ,  C12N15/63

    CPC分类号： C12N9/1294 ,  C12Q1/48 ,  G01N2333/91295

    摘要： Disclosed are a pyruvate orthophosphate dikinase gene coding for a polypeptide containing the amino acid sequence of SEQ ID NO:1 or an amino acid sequence where in said amino acid sequence one or more amino acids are added, deleted or substituted and bringing about the activity of pyruvate orthophosphate dikinase; recombinant DNA having the pyruvate orthophosphate dikinase gene integrated in vector DNA; and a process for producing pyruvate orthophosphate dikinase, which comprises culturing a microorganism belonging to the genus Escherichia carrying the above recombinant DNA in a medium, and recovering pyruvate orthophosphate dikinase from the resulting culture. The pyruvate orthophosphate dikinase gene and a process for producing pyruvate orthophosphate dikinase are provided.

    摘要翻译： 公开了编码含有SEQ ID NO：1的氨基酸序列的多肽的丙酮酸正磷酸二激酶基因或氨基酸序列，其中在所述氨基酸序列中添加一个或多个氨基酸，缺失或取代并产生 丙酮酸正磷酸二激酶; 具有整合在载体DNA中的丙酮酸正磷酸二激酶基因的重组DNA; 以及生产丙酮酸正磷酸二激酶的方法，其包括在培养基中培养属于携带上述重组DNA的埃希氏杆菌属的微生物，并从所得培养物中回收丙酮酸正磷酸二激酶。 提供丙酮酸正磷酸二激酶基因和制备丙酮酸正磷酸二激酶的方法。

    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member
    4.
    发明授权
    Luminescent tool, its auxiliary member and method of preserving bioluminescent composition used in the tool and the auxiliary member 失效
    发光工具，其辅助构件和保存在工具中使用的生物发光组合物和辅助构件的方法

    公开(公告)号：US06521304B1

    公开(公告)日：2003-02-18

    申请号：US09331292

    申请日：1999-06-18

    申请人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    发明人： Naoki Kajiyama ,  Ayumi Arai ,  Eiichi Nakano ,  Hiroki Tatsumi ,  Teruo Watarai ,  Naoki Eisaki ,  Tatsuya Sakakibara ,  Masaru Suzuki

    IPC分类号： F21K206

    CPC分类号： C09K11/07 ,  F21K2/06

    摘要： The present invention relates to luminescent playthings such as candles, table lights, pen lights, illumination, illuminating playthings for camp, illuminating playthings for night fishing, fish-gathering lamps, safety candles, neon lights, luminescent inks, luminescent pens, luminescent coatings or luminescent writing implements, in which a soft light of an indescribable tone of color not obtainable from flames of conventional candles is emitted via seawater, lake and marsh water, river water, ground water, tap water or mineral drinking water by utilizing 3 components of luciferin, luciferase and ATP or 4 components of these components plus a metal salt, out of components necessary for bioluminescence in fireflies.

    摘要翻译： 本发明涉及诸如蜡烛，台灯，笔灯，照明，营地的照明玩具，夜间钓鱼的照明玩具，聚光灯，安全蜡烛，霓虹灯，发光油墨，发光笔，发光涂层或发光的发光玩具 发光书写工具，其中可以通过海水，湖泊和沼泽水，河水，地下水，自来水或矿物饮用水通过利用荧光素3的三种成分从普通蜡烛的火焰中获得难以形容的色调的柔和光线 ，荧光素酶和ATP或这些组分的4种组分加上金属盐，不仅仅是萤火虫生物发光所必需的组分。

    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
    5.
    发明授权
    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method 失效

    公开(公告)号：US5843746A

    公开(公告)日：1998-12-01

    申请号：US782118

    申请日：1997-01-13

    申请人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    发明人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    IPC分类号： C12N15/09 ,  C07K19/00 ,  C12N1/21 ,  C12N9/02 ,  C12Q1/26 ,  C12Q1/66 ,  C12R1/185 ,  C12R1/19 ,  C12N15/62 ,  C12N15/53 ,  C12N15/63

    CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C07K2319/00

    摘要： The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor by the use of said biotinated firefly luciferase. According to the present invention, there are provided biotinated firefly luciferase, a biotinated firefly luciferase gene, a recombinant DNA, a process for producing biotinated firefly luciferase and a bioluminescent analysis method. The present invention enables the efficient production of biotinated firefly luciferase of uniform properties with the uniform structure of firefly luciferase in which one biotin molecule has been bound to a specific residue and whose activity is hardly lost by biotination and not lost even binding to streptoavidin or avidin, and the biotinated firefly luciferase of constant properties obtained in the present invention permits highly sensitive measurements in bioluminescent analysis as compared with the conventional chemically modified biotinated firefly luciferase so that the present invention is industrially extremely useful.

    Purified luciferase from luciola lateralis
    6.
    发明授权
    Purified luciferase from luciola lateralis 失效
    来自luciola lateralis的纯化萤光素酶

    公开(公告)号：US5352598A

    公开(公告)日：1994-10-04

    申请号：US759814

    申请日：1991-08-29

    申请人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    发明人： Naoki Kajiyama ,  Tsutomu Masuda ,  Hiroki Tatsumi ,  Eiichi Nakano

    IPC分类号： C12N9/02 ,  C12N1/00

    CPC分类号： C12N9/0069 ,  C12Y113/12007 ,  Y10S435/815 ,  Y10S435/816

    摘要： A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.

    摘要翻译： 公开了一种来自Luciola lateralis的纯化的萤光素酶。 该酶的特征在于具有以下特性：最佳pH范围为7.5至9.5，最适温度范围为0℃至50℃，并且该酶不作用于ADP，CTP，UTP和GTP。 通过使用包括凝胶过滤色谱法，羟基磷灰石柱色谱法和三（羟基）氨基甲烷 - 氢氯酸缓冲液的方法纯化酶。

    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
    8.
    发明授权
    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method 失效

    公开(公告)号：US5814465A

    公开(公告)日：1998-09-29

    申请号：US460934

    申请日：1995-06-05

    申请人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    发明人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    IPC分类号： C12N15/09 ,  C07K19/00 ,  C12N1/21 ,  C12N9/02 ,  C12Q1/26 ,  C12Q1/66 ,  C12R1/185 ,  C12R1/19 ,  C12N9/96 ,  G01N33/566

    CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C07K2319/00

    摘要： The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor by the use of said biotinated firefly luciferase. According to the present invention, there are provided biotinated firefly luciferase, a biotinated firefly luciferase gene, a recombinant DNA, a process for producing biotinated firefly luciferase and a bioluminescent analysis method. The present invention enables the efficient production of biotinated firefly luciferase of uniform properties with the uniform structure of firefly luciferase in which one biotin molecule has been bound to a specific residue and whose activity is hardly lost by biotination and not lost even binding to streptoavidin or avidin, and the biotinated firefly luciferase of constant properties obtained in the present invention permits highly sensitive measurements in bioluminescent analysis as compared with the conventional chemically modified biotinated firefly luciferase so that the present invention is industrially extremely useful.