公开(公告)号：US09217743B2

公开(公告)日：2015-12-22

申请号：US13498067

申请日：2010-10-18

申请人： Hiroshi Handa ,  Hideki Ando ,  Takumi Itoh ,  Kentaro Hotta

发明人： Hiroshi Handa ,  Hideki Ando ,  Takumi Itoh ,  Kentaro Hotta

IPC分类号： G01N33/68 ,  A01K67/027 ,  C07K14/435 ,  C12N15/12 ,  G01N33/566

CPC分类号： G01N33/566 ,  G01N2500/04

摘要： With an aim to provide means for developing a compound devoid of teratogenicity but retaining beneficial actions, a screening method for a non-teratogenic substance comprising bringing a test substance into contact with cereblon or a fragment of cereblon, evaluating the bindability of the test substance with cereblon or the fragment of cereblon, and selecting a test substance that does not bind to cereblon or the fragment of cereblon or a test substance exhibiting lower bindability with cereblon or the fragment of cereblon than does thalidomide is provided.

摘要翻译： 目的是提供开发不具有致畸性但保留有益作用的化合物的方法，用于非致畸物质的筛选方法，包括使测试物质与大脑或脑片的片段接触，评估测试物质与 提供了与沙门氏菌相比，不能结合到脑部或与本发明的化合物相比较的表现出与巧克力片或巧克力片相比具有较低粘合性的测试物质的测试物质。

公开(公告)号：US09164420B2

公开(公告)日：2015-10-20

申请号：US14092114

申请日：2013-11-27

申请人： Hiroshi Handa

发明人： Hiroshi Handa

IPC分类号： G03G15/08

CPC分类号： G03G15/0817

摘要： A developing device including: a housing; a developing roller including a roller main body having a recess portion recessed inwards in an axis line direction, the recess portion including, a first surface extending in the axis line direction, and a second surface extending from an inner end portion of the first surface towards a direction intersecting with the axis line direction; and a seal member arranged between the housing and the roller main body, wherein, in the axis line direction, the second surface is positioned within a range in which the seal member is arranged, and wherein, in the axis line direction, a distance from an inner end portion of the seal member to the second surface is larger than a length of a part at which the seal member and the first surface overlap with each other.

摘要翻译： 一种显影装置，包括：壳体; 一种显影辊，包括一个辊主体，该辊主体具有沿轴线方向向内凹进的凹陷部分，该凹部包括沿轴线方向延伸的第一表面和从第一表面的内端部延伸的第二表面朝向 与轴线方向相交的方向; 以及设置在所述壳体和所述辊主体之间的密封构件，其中，在所述轴线方向上，所述第二面位于所述密封构件的排列的范围内，并且其中，在所述轴线方向上， 密封构件的第二表面的内端部大于密封构件和第一表面彼此重叠的部分的长度。

公开(公告)号：US20140189895A1

公开(公告)日：2014-07-03

申请号：US14234060

申请日：2012-07-17

申请人： Tadashi Wada ,  Kei Takeda ,  Hiroshi Handa

发明人： Tadashi Wada ,  Kei Takeda ,  Hiroshi Handa

IPC分类号： C12N15/11

CPC分类号： C12N15/111 ,  A01K67/027 ,  A01K2207/05 ,  A01K2227/40 ,  A01K2267/03 ,  C12N15/63 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/3233

摘要： The present invention provides a method of artificially repressing gene expression, which is simpler to design than conventional methods (the RNAi, ribozyme and antisense methods) and which allows for easier confirmation of the effect.A method of inhibiting the translation reaction of a target gene, comprising cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA is provided. Also provided are a kit for inhibiting the translation reaction of a target gene, comprising a reagent capable of cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA; a cell in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA; and a non-human organism in which the translation reaction of a target gene is inhibited by introduction thereinto of a reagent capable of cutting out a part of the poly(A) tail and/or 3′-terminal sequence of the target mRNA.

摘要翻译： 本发明提供人工抑制基因表达的方法，其比常规方法（RNAi，核酶和反义方法）设计得更简单，并且允许更容易地确认效果。 提供了抑制靶基因的翻译反应的方法，其包括切下部分目标mRNA的poly（A）尾和/或3'-末端序列。 还提供了用于抑制靶基因的翻译反应的试剂盒，其包含能够切出靶mRNA的多（A）尾和/或3'末端序列的一部分的试剂; 通过向其中引入能够切出部分目标mRNA的多（A）尾和/或3'末端序列的试剂而抑制靶基因的翻译反应的细胞; 和通过向其中引入能够切割出部分目标mRNA的聚（A）尾和/或3'-末端序列的试剂而抑制靶基因的翻译反应的非人生物体。

公开(公告)号：US20140153962A1

公开(公告)日：2014-06-05

申请号：US14092114

申请日：2013-11-27

申请人： Hiroshi Handa

发明人： Hiroshi Handa

IPC分类号： G03G15/08

CPC分类号： G03G15/0817

摘要： A developing device including: a housing; a developing roller including a roller main body having a recess portion recessed inwards in an axis line direction, the recess portion including, a first surface extending in the axis line direction, and a second surface extending from an inner end portion of the first surface towards a direction intersecting with the axis line direction; and a seal member arranged between the housing and the roller main body, wherein, in the axis line direction, the second surface is positioned within a range in which the seal member is arranged, and wherein, in the axis line direction, a distance from an inner end portion of the seal member to the second surface is larger than a length of a part at which the seal member and the first surface overlap with each other.

摘要翻译： 一种显影装置，包括：壳体; 一种显影辊，包括一个辊主体，该辊主体具有沿轴线方向向内凹进的凹陷部分，该凹部包括沿轴线方向延伸的第一表面和从第一表面的内端部延伸的第二表面朝向 与轴线方向相交的方向; 以及设置在所述壳体和所述辊主体之间的密封构件，其中，在所述轴线方向上，所述第二面位于所述密封构件的排列的范围内，并且其中，在所述轴线方向上， 密封构件的第二表面的内端部大于密封构件和第一表面彼此重叠的部分的长度。

公开(公告)号：US20140153961A1

公开(公告)日：2014-06-05

申请号：US14091941

申请日：2013-11-27

申请人： Hiroshi Handa ,  Soun Kanada

发明人： Hiroshi Handa ,  Soun Kanada

IPC分类号： G03G15/08

CPC分类号： G03G15/0817

摘要： A developing device including: a first seal member arranged between a housing and a layer thickness regulation blade, and a second seal member arranged between the housing and a developing roller, wherein the first seal member is arranged adjacent to the second seal member in a rotating direction of the developing roller, wherein the second seal member includes a first member contacting a circumferential surface of the developing roller and a second member arranged between the housing and the first member, wherein the second member includes a protruding portion that, in the rotating direction of the developing roller, protrudes more towards the first seal member than the first member, and wherein end portions of the first and second members in the rotating direction of the developing roller are respectively configured to contact with the first seal member.

摘要翻译： 一种显影装置，包括：布置在壳体和层厚度调节刀片之间的第一密封构件和布置在壳体和显影辊之间的第二密封构件，其中第一密封构件以旋转的方式布置成与第二密封构件相邻 显影辊的方向，其中所述第二密封构件包括接触所述显影辊的圆周表面的第一构件和布置在所述壳体和所述第一构件之间的第二构件，其中所述第二构件包括突出部分，所述突出部分沿所述旋转方向 显影辊的第一和第二构件的端部分别构造成与第一密封构件接触。

公开(公告)号：US08399022B2

公开(公告)日：2013-03-19

申请号：US12522178

申请日：2007-12-28

申请人： Masanori Abe ,  Hiroshi Handa ,  Takashi Nakagawa ,  Masaru Tada ,  Ryuichi Shimazu ,  Toshiyuki Tanaka

发明人： Masanori Abe ,  Hiroshi Handa ,  Takashi Nakagawa ,  Masaru Tada ,  Ryuichi Shimazu ,  Toshiyuki Tanaka

IPC分类号： A61K9/14

CPC分类号： C01G49/0018 ,  A61K9/5094 ,  A61K9/5115 ,  A61K9/5192 ,  A61K41/0052 ,  A61K47/6923 ,  A61K49/1887 ,  B82Y5/00 ,  B82Y25/00 ,  B82Y30/00 ,  C01G49/0027 ,  C01P2004/51 ,  C01P2004/62 ,  C01P2004/64 ,  C01P2006/22 ,  C01P2006/42 ,  H01F1/0054 ,  H01F1/36 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/2993 ,  Y10T428/2995 ,  Y10T428/2998

摘要： A method for producing spherical ferrite nanoparticles includes the steps of: preparing a first aqueous solution containing a disaccharide, an alkaline, an oxidation agent, seed particles and divalent iron ions; and conducting particle growth in the first aqueous solution to produce the spherical ferrite nanoparticles.

摘要翻译： 制备球形铁氧体纳米颗粒的方法包括以下步骤：制备含有二糖，碱，氧化剂，种子颗粒和二价铁离子的第一水溶液; 并在第一水溶液中进行颗粒生长以产生球形铁氧体纳米颗粒。

公开(公告)号：US20110135718A1

公开(公告)日：2011-06-09

申请号：US12849437

申请日：2010-08-03

申请人： Hiroshi HANDA ,  Akira Nakanishi ,  Masaaki Kawano

发明人： Hiroshi HANDA ,  Akira Nakanishi ,  Masaaki Kawano

IPC分类号： A61K9/48 ,  C12P21/00 ,  A61K48/00 ,  A61P43/00

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5258 ,  C07K14/005 ,  C12N2710/22022 ,  C12N2710/22023 ,  Y02A50/467

摘要： There is provided a novel method of forming uniform viral particles under physiological conditions. The method of forming uniform-sized viral particle aggregates composed of viral protein is characterized by incubating a viral protein such as SV40 virus VP1 at pH 5.0 to 7.0, room temperature, in the presence of 130 mM to 170 mM sodium chloride and 1.5 mM to 2.5 mM divalent cation, and in the presence of a particle formation acceleration factor such as SV40 VP2. For encapsulation of a substance to be encapsulated in the viral particles, the substance to be encapsulated is included during the incubation.

摘要翻译： 提供了一种在生理条件下形成均匀病毒颗粒的新方法。 形成由病毒蛋白组成的均匀尺寸的病毒颗粒聚集体的方法的特征在于在室温下，在130mM至170mM氯化钠和1.5mM的存在下，将病毒蛋白如SV40病毒VP1在pH 5.0至7.0温度下孵育至 2.5mM二价阳离子，并且在存在颗粒形成加速因子如SV40 VP2的情况下。 为了封装待包封在病毒颗粒中的物质，在孵育期间包括待包被的物质。

公开(公告)号：US20100239864A1

公开(公告)日：2010-09-23

申请号：US12294770

申请日：2006-03-27

申请人： Hiroshi Handa ,  Kosuke Nishio ,  Nobuyuki Gokon ,  Masanori Abe

发明人： Hiroshi Handa ,  Kosuke Nishio ,  Nobuyuki Gokon ,  Masanori Abe

IPC分类号： B32B19/02

CPC分类号： C09D125/14 ,  C08F2/24 ,  C08F2/44 ,  C08F212/08 ,  C08F212/36 ,  C08F292/00 ,  C08F2220/325 ,  C08K3/22 ,  C08K2003/2275 ,  G01N33/5434 ,  Y10T428/2998 ,  C08F220/32

摘要： Polymer coated ferromagnetic particles could be obtained giving a solution to a problem of difficulty in dispersing ferromagnetic fine particles to a level of monodispersion because of their properties apt to form aggregates. Providing hydrophilic character to hydrophobic ferromagnetic particles obtained by adsorbing hydrophobizing agent to hydrophilic ferromagnetic particles, and dispersed using nonionic surface activating agent suppressing ionic strength increase of the particles. Monomer liquid was emulsified using nonionic surface activating agent and ionic surface activating agent. Mixing the dispersion liquid of ferromagnetic particles and the monomer emulsion were mixed and emulsion polymerization was conducted. Then well-dispersed homogeneous and stable polymer coated ferromagnetic particles showing good response to magnetic field were obtained.

摘要翻译： 可以获得聚合物涂覆的铁磁性颗粒，从而提供难以将铁磁性细颗粒分散到单分散水平的问题的解决方案，因为它们的性质易于形成聚集体。 对通过将疏水化剂吸附到亲水性铁磁颗粒上获得的疏水性铁磁颗粒提供亲水性，并使用抑制颗粒的离子强度增加的非离子表面活化剂分散。 使用非离子表面活化剂和离子表面活化剂将单体液体乳化。 将铁磁颗粒和单体乳液的分散液混合并进行乳液聚合。 然后获得了良好分散的均匀且稳定的聚合物涂覆的铁磁性颗粒，其表现出对磁场的良好响应。

公开(公告)号：US20050084539A1

公开(公告)日：2005-04-21

申请号：US10503544

申请日：2003-02-03

申请人： Hiroshi Handa ,  Masanori Abe ,  Makoto Hasegawa

发明人： Hiroshi Handa ,  Masanori Abe ,  Makoto Hasegawa

IPC分类号： C01G49/06 ,  C09C1/24 ,  A61K9/16 ,  A61K9/50 ,  A61K33/26

CPC分类号： B82Y30/00 ,  C01G49/06 ,  C01P2002/70 ,  C01P2004/04 ,  C01P2004/64 ,  C01P2006/42 ,  C09C1/24

摘要： A ferrite bonded organic substance having that has a strong bond between organic substance and ferrite can be obtained by carrying out formation and bonding of a ferrite to an organic substance having a functional group selected from a carboxyl group, a mercapto group and a mercapto group in oxidized form. In the producing method of manufacturing the ferrite bonded organic substance, ferrite plating can be employed. Wide application in, for example, various drugs, bio-analytical reagents, DNA, various contrast media and MRI sensitizers that permit magnetic manipulation can be realized by bonding ferrite to organic substance retaining a bio-active substance.

摘要翻译： 通过使铁素体与具有选自羧基，巯基和巯基的官能团的有机物的形成和结合，可以得到有机物质与铁素体之间具有强键的铁氧体键合有机物质 氧化形式。 在铁氧体键合有机物的制造方法中，可以使用铁素体电镀。 可以通过将铁氧体结合到保留生物活性物质的有机物质上，实现例如各种药物，生物分析试剂，DNA，各种造影剂和允许磁操作的MRI敏化剂的广泛应用。

公开(公告)号：US06703207B2

公开(公告)日：2004-03-09

申请号：US10003014

申请日：2001-11-02

申请人： Hiroshi Handa ,  Haruma Kawaguchi

发明人： Hiroshi Handa ,  Haruma Kawaguchi

IPC分类号： G01N3353

CPC分类号： G01N33/54313 ,  C07K14/705 ,  G01N33/54353 ,  G01N33/94 ,  Y10S435/803 ,  Y10S435/815 ,  Y10S530/815 ,  Y10S530/816

摘要： A compound possessing physiological activity is coupled to a styrene-glycidyl methacrylate polymer through a spacer. Compounds that may be used include receptors such as proteins, and 3-[(5-(2,3-dimethoxy-6-methyl-benzoquinonyl)]-2-nonyl-2-propionic a preferred spacer is an ethylene glycol diglycidyl ether derivative. Preferably, the whole surface of the styrene-glycidyl methacrylate polymer in microsphere form is covered with glycidyl methacrylate. The microsphere may be used for isolating and detecting substances such as proteins that bind to the coupled compound.

摘要翻译： 具有生理活性的化合物通过间隔物与苯乙烯 - 甲基丙烯酸缩水甘油酯聚合物偶联。 可以使用的化合物包括蛋白质等受体，和3 - [（5-（2,3-二甲氧基-6-甲基 - 苯醌基）] -2-壬基-2-丙酸的优选间隔物是乙二醇二缩水甘油醚衍生物 优选地，以微球形式的苯乙烯 - 甲基丙烯酸缩水甘油酯聚合物的整个表面被甲基丙烯酸缩水甘油酯覆盖。微球可以用于分离和检测诸如与偶联化合物结合的蛋白质的物质。