利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

59 条记录 (耗时 0.029 秒)

    METHOD FOR PRODUCING SIALIC-ACID-CONTAINING SUGAR CHAIN
    4.
    发明申请
    METHOD FOR PRODUCING SIALIC-ACID-CONTAINING SUGAR CHAIN 有权
    生产含酸性糖的糖链的方法

    公开(公告)号：US20140066617A1

    公开(公告)日：2014-03-06

    申请号：US14002645

    申请日：2012-02-27

    申请人： Yasunori Chiba ,  Yoshie Takahashi ,  Hisashi Narimatsu ,  Kazuhiro Fukae

    发明人： Yasunori Chiba ,  Yoshie Takahashi ,  Hisashi Narimatsu ,  Kazuhiro Fukae

    IPC分类号： C12P19/18 ,  C08B37/00

    CPC分类号： C08B37/0063 ,  C08B37/006 ,  C12N9/1081 ,  C12N15/09 ,  C12N15/52 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26 ,  C12P21/005 ,  C12Y204/99 ,  C12Y204/99001 ,  C12Y301/03

    摘要： [Problem to be Solved]The importance of sugar chains having α2,3- or α2,6-linked sialic acid at their non-reducing ends is known. Industrial production has been demanded for these sugar chain compounds. Particularly, the production of glycoprotein drugs or the like inevitably requires producing in quantity sugar chains having homogeneous structures by controlling the linking pattern (α2,6-linkage or α2,3-linkage) of sialic acid. Particularly, a triantennary or tetraantennary N-type complex sugar chain having sialic acid at each of all non-reducing ends is generally considered difficult to chemically synthesize. There has been no report disclosing that such a sugar chain was chemically synthesized. Furthermore, these sugar chains are also difficult to efficiently prepare enzymatically.[Solution]The present inventors have newly found the activity of sialyltransferase of degrading sialic acid on a reaction product in the presence of CMP and also found that formed CMP can be degraded enzymatically to thereby efficiently produce a sialic acid-containing sugar chain. The present inventors have further found that even a tetraantennary N-type sugar chain having four α2,6-linked sialic acid molecules, which has previously been difficult to synthesize, can be prepared at high yields by one-pot synthesis comprising the elongation reaction of a biantennary sugar chain used as a starting material without performing purification after each enzymatic reaction.

    摘要翻译： [待解决的问题]已知具有α2,3-或α2,6-连接的唾液酸在其非还原末端的糖链的重要性。 这些糖链化合物已经需要工业生产。 特别地，糖蛋白药物等的生产不可避免地需要通过控制唾液酸的连接模式（α2,6-键或α2,3-键）来产生具有均匀结构的量的糖链。 特别地，在所有非还原性末端中的每一个具有唾液酸的三末端或四末端N-型复合糖链通常被认为难以化学合成。 没有报道披露这样的糖链是化学合成的。 此外，这些糖链也难以有效地制备酶。 [解决方案]本发明人在CMP存在下新发现唾液酸转移酶唾液酸转移酶在反应产物上的活性，并且发现形成的CMP可以酶促降解，从而有效地产生含唾液酸的糖链。 本发明人进一步发现，即使具有四个α2,6-连接的唾液酸分子的四末端N-型糖链，其先前难以合成，可以通过一锅合成以高收率制备，其包括延伸反应 在每个酶反应后不用进行纯化的双天线糖链用作原料。

    Oligosaccharides fragmentation pattern analysis system and oligosaccharides fragmentation pattern analysis method
    8.
    发明申请
    Oligosaccharides fragmentation pattern analysis system and oligosaccharides fragmentation pattern analysis method 审中-公开
    寡糖片段化分析方法和寡糖片段分析方法

    公开(公告)号：US20060041388A1

    公开(公告)日：2006-02-23

    申请号：US11207005

    申请日：2005-08-19

    申请人： Kazuhiko Fukui ,  Katsutoshi Takahashi ,  Yuri Mukai ,  Akihiko Kameyama ,  Hisashi Narimatsu

    发明人： Kazuhiko Fukui ,  Katsutoshi Takahashi ,  Yuri Mukai ,  Akihiko Kameyama ,  Hisashi Narimatsu

    IPC分类号： G06F19/00

    CPC分类号： H01J49/0036

    摘要： Oligosaccharides fragmentation pattern analysis system 10 includes: a geometry optimization part for calculating an optimized structure of an ionized oligosaccharide; a binding parameter extraction part for extracting multiple kinds of binding parameters which relate to bond strengths for respective multiple candidate bonds to be the candidate of the cleavage site within the oligosaccharide from the calculation results by the geometry optimization part; a parameter conversion part for converting respective multiple kinds of binding parameters extracted at the binding parameter extraction part, into binding scores; a weight information storage part for storing the weight information representing contribution to respective bond strengths of multiple kinds of binding scores; and a total score calculating part for determining total score of multiple kinds of binding scores in each candidate bond such that the multiple kinds of binding parameters are weighed according to the weight information.

    摘要翻译： 寡糖分解模式分析系统10包括：用于计算电离寡糖的优化结构的几何优化部分; 一种结合参数提取部分，用于从几何优化部分的计算结果中提取与各多个候选键的键强度相关的多种结合参数，作为寡糖中切割位点的候选者; 用于将在所述绑定参数提取部提取的各种绑定参数转换为绑定分数的参数转换部; 权重信息存储部分，用于存储表示对多种绑定分数的各自的绑定强度的贡献的权重信息; 以及总分计算部分，用于确定每个候选键中的多种结合分数的总分，使得根据权重信息称重多种结合参数。