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公开(公告)号:US20090093025A1
公开(公告)日:2009-04-09
申请号:US11809528
申请日:2007-06-01
申请人: Sierd Bron , Jan D.H. Jongbloed , Joerg Muller , Jan M. Van Dijl
发明人: Sierd Bron , Jan D.H. Jongbloed , Joerg Muller , Jan M. Van Dijl
CPC分类号: C07K14/32 , C07K2319/034 , C12N15/625 , C12N15/70 , C12N15/75 , C12P21/02
摘要: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
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公开(公告)号:US20080166757A1
公开(公告)日:2008-07-10
申请号:US11809669
申请日:2007-06-01
申请人: Sierd Bron , Jan D.H. Jongbloed , Joerg Muller , Jan M. Van Dijl
发明人: Sierd Bron , Jan D.H. Jongbloed , Joerg Muller , Jan M. Van Dijl
CPC分类号: C07K14/32 , C07K2319/034 , C12N15/625 , C12N15/70 , C12N15/75 , C12P21/02
摘要: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
摘要翻译: 本文描述了增强宿主细胞中蛋白质分泌的方法。 在优选的实施方案中,宿主细胞是革兰氏阳性微生物如芽孢杆菌。 在另一个优选的实施方案中,宿主细胞是革兰氏阴性微生物。 优选地,革兰氏阴性微生物是大肠杆菌或Pantoaea属的成员。 蛋白质分泌可能通过Tat途径的蛋白质组分的过度表达来增强。 或者,通过形成包含tat信号序列和感兴趣的蛋白质的嵌合多肽,可以选择性地增强外来蛋白质的分泌。 在优选的实施方案中,tat信号序列选自phoD或LipA。
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公开(公告)号:US5246838A
公开(公告)日:1993-09-21
申请号:US662005
申请日:1991-02-28
IPC分类号: C12N1/21 , C12N9/28 , C12N9/52 , C12N9/56 , C12N9/86 , C12N15/09 , C12N15/57 , C12N15/62 , C12N15/67 , C12N15/70 , C12N15/75 , C12R1/125 , C12R1/19 , C12R1/42
CPC分类号: C12N9/2417 , C12N15/625 , C12N15/67 , C12N15/70 , C12N15/75 , C12N9/52 , C12N9/86 , C07K2319/02 , C07K2319/034 , C07K2319/036 , C07K2319/61
摘要: The subject invention describes the cloning and overexpression of leader peptidase genes. A method for isolating a leader peptidase gene is disclosed. Overexpression of the signal peptidase in a suitable host species leads to an enhanced rate of protein processing.
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公开(公告)号:US07316924B2
公开(公告)日:2008-01-08
申请号:US09954737
申请日:2001-09-17
申请人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
发明人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
CPC分类号: C07K14/32 , C07K2319/034 , C12N15/625 , C12N15/70 , C12N15/75 , C12P21/02
摘要: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
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公开(公告)号:US07553637B2
公开(公告)日:2009-06-30
申请号:US10500660
申请日:2002-12-12
申请人: Sierd Bron , Harold Tjalsma , Jan M. Van Dijl
发明人: Sierd Bron , Harold Tjalsma , Jan M. Van Dijl
CPC分类号: C07K14/32
摘要: The present invention provides methods and compositions for the efficient and enhanced secretion of a protein of interest from a host cell. In specific, proteins are secreted through the Sec-dependent pathway, involving the spoIIIJ and/or yqjG gene product(s). In some embodiments, expression of the spoIIIJ and/or yqjG gene product(s) is modulated by a promoter operably linked to the gene.
摘要翻译: 本发明提供了用于从宿主细胞有效和增强目的蛋白质分泌的方法和组合物。 具体而言,蛋白质通过Sec依赖性途径分泌,涉及spoIIIJ和/或yqjG基因产物。 在一些实施方案中,spoIIIJ和/或yqjG基因产物的表达由可操作地连接到该基因的启动子调节。
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公开(公告)号:US20080248525A1
公开(公告)日:2008-10-09
申请号:US11809756
申请日:2007-06-01
申请人: Sierd Bron , Jan D.H. Jongbloed , Joerg Muller , Jan M. Van Dijl
发明人: Sierd Bron , Jan D.H. Jongbloed , Joerg Muller , Jan M. Van Dijl
CPC分类号: C07K14/32 , C07K2319/034 , C12N15/625 , C12N15/70 , C12N15/75 , C12P21/02
摘要: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coil or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
摘要翻译: 本文描述了增强宿主细胞中蛋白质分泌的方法。 在优选的实施方案中,宿主细胞是革兰氏阳性微生物如芽孢杆菌。 在另一个优选的实施方案中,宿主细胞是革兰氏阴性微生物。 优选地,革兰氏阴性微生物是大肠杆菌或Pantoaea属的成员。 蛋白质分泌可能通过Tat途径的蛋白质组分的过度表达来增强。 或者,通过形成包含tat信号序列和感兴趣的蛋白质的嵌合多肽,可以选择性地增强外来蛋白质的分泌。 在优选的实施方案中,tat信号序列选自phoD或LipA。
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公开(公告)号:US07897742B2
公开(公告)日:2011-03-01
申请号:US11809756
申请日:2007-06-01
申请人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
发明人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
CPC分类号: C07K14/32 , C07K2319/034 , C12N15/625 , C12N15/70 , C12N15/75 , C12P21/02
摘要: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
摘要翻译: 本文描述了增强宿主细胞中蛋白质分泌的方法。 在优选的实施方案中,宿主细胞是革兰氏阳性微生物如芽孢杆菌。 在另一个优选的实施方案中,宿主细胞是革兰氏阴性微生物。 优选地,革兰氏阴性微生物是大肠杆菌或泛菌属的成员。 蛋白质分泌可能通过Tat途径的蛋白质组分的过度表达来增强。 或者,通过形成包含tat信号序列和感兴趣的蛋白质的嵌合多肽,可以选择性地增强外来蛋白质的分泌。 在优选的实施方案中,tat信号序列选自phoD或LipA。
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公开(公告)号:US07884192B2
公开(公告)日:2011-02-08
申请号:US11809669
申请日:2007-06-01
申请人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
发明人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
CPC分类号: C07K14/32 , C07K2319/034 , C12N15/625 , C12N15/70 , C12N15/75 , C12P21/02
摘要: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
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公开(公告)号:US07884191B2
公开(公告)日:2011-02-08
申请号:US11809528
申请日:2007-06-01
申请人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
发明人: Sierd Bron , Jan D. H. Jongbloed , Joerg Muller , Jan M. Van Dijl
CPC分类号: C07K14/32 , C07K2319/034 , C12N15/625 , C12N15/70 , C12N15/75 , C12P21/02
摘要: Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
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