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    Plastid Transformation System to Prevent the Intramolecular Recombination of Transgene
    2.
    发明申请
    Plastid Transformation System to Prevent the Intramolecular Recombination of Transgene 失效
    质体转化体系，以防止转基因的分子内重组

    公开(公告)号：US20080301834A1

    公开(公告)日：2008-12-04

    申请号：US12159595

    申请日：2006-10-25

    申请人： Jang Ryol Liu ,  Hwa-Jee Chung ,  Sung Ran Min ,  Won Joong Jeong ,  Ju Young Park

    发明人： Jang Ryol Liu ,  Hwa-Jee Chung ,  Sung Ran Min ,  Won Joong Jeong ,  Ju Young Park

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8214

    摘要： The present invention relates to a plastid transformation system capable of preventing second recombination events of heterologous genes inserted into plastid genomes. More specifically, this invention relates to a plastid transformation vector carrying a promoter and a terminator derived from organisms other than tobacco. The inventive recombinant expression vector for plastid transformation is capable of mass-producing exogenous proteins on a level par with conventional vectors carrying promoter/terminator couples of tobacco origin. At the same time, it is capable of preventing second recombination events within plastids. Thus, the vector of this invention is greatly useful in producing transgenic plants since it can effect a secure introduction of heterologous genes and support normal transformation and heterologous gene expression.

    摘要翻译： 本发明涉及能够防止插入到质体基因组中的异源基因的第二重组事件的质体转化系统。 更具体地，本发明涉及携带启动子的质体转化载体和源自烟草以外的生物体的终止子。 本发明的用于质体转化的重组表达载体能够以与产生烟草来源的启动子/终止子对的常规载体水平相当的量产生外源蛋白质。 同时，它能够防止质体内的第二次重组事件。 因此，本发明的载体在生产转基因植物中是非常有用的，因为它可以有效地引入异源基因并支持正常转化和异源基因表达。

    Plastid transformation system to prevent the intramolecular recombination of transgene
    4.
    发明授权
    Plastid transformation system to prevent the intramolecular recombination of transgene 失效
    质体转化系统，以防止转基因的分子内重组

    公开(公告)号：US07888562B2

    公开(公告)日：2011-02-15

    申请号：US12159595

    申请日：2006-10-25

    申请人： Jang Ryol Liu ,  Hwa-Jee Chung ,  Sung Ran Min ,  Won Joong Jeong ,  Ju Young Park

    发明人： Jang Ryol Liu ,  Hwa-Jee Chung ,  Sung Ran Min ,  Won Joong Jeong ,  Ju Young Park

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8214

    摘要： The present invention relates to a plastid transformation system capable of preventing second recombination events of heterologous genes inserted into plastid genomes. More specifically, this invention relates to a plastid transformation vector carrying a promoter and a terminator derived from organisms other than tobacco. The inventive recombinant expression vector for plastid transformation is capable of mass-producing exogenous proteins on a level par with conventional vectors carrying promoter/terminator couples of tobacco origin. At the same time, it is capable of preventing second recombination events within plastids. Thus, the vector of this invention is greatly useful in producing transgenic plants since it can effect a secure introduction of heterologous genes and support normal transformation and heterologous gene expression.

    摘要翻译： 本发明涉及能够防止插入到质体基因组中的异源基因的第二重组事件的质体转化系统。 更具体地，本发明涉及携带启动子的质体转化载体和源自烟草以外的生物体的终止子。 本发明的用于质体转化的重组表达载体能够以与产生烟草来源的启动子/终止子对的常规载体水平相当的量产生外源蛋白质。 同时，它能够防止质体内的第二次重组事件。 因此，本发明的载体在生产转基因植物中是非常有用的，因为它可以有效地引入异源基因并支持正常转化和异源基因表达。

    Nucleic acid encoding chitin-binding receptor kinase
    7.
    发明授权
    Nucleic acid encoding chitin-binding receptor kinase 失效
    核酸编码几丁质结合受体激酶

    公开(公告)号：US06410706B1

    公开(公告)日：2002-06-25

    申请号：US09503922

    申请日：2000-02-14

    申请人： Hyun-Sook Pai ,  Jang-Ryol Liu ,  Hye-Sun Cho ,  Youn-Sung Kim

    发明人： Hyun-Sook Pai ,  Jang-Ryol Liu ,  Hye-Sun Cho ,  Youn-Sung Kim

    IPC分类号： C12N1552

    CPC分类号： C07K14/415 ,  C12N9/2442 ,  C12N15/8282 ,  C12N15/8283 ,  C12Y302/01014

    摘要： The present invention provides a novel receptor kinase and the gene thereof which can be used for activating plant defense systems against several pathogens such as fungi. The receptor kinase CHRK1 of this present invention has an extracellular domain similar to a chitinase, and whose gene expression is stimulated by infection of TMV. In addition, the receptor kinase CHRK1 contains a chitin-binding activity as well as a kinase activity so that it binds to chitin of fungal cell wall as a chitin receptor, stimulates a kinase domain, and thus effectively activates versatile plant defense systems. Therefore, the receptor kinase CHRK1 of this present invention can be used for developing plants having high resistance to fungi.

    摘要翻译： 本发明提供了可用于激活针对若干病原体如真菌的植物防御系统的新型受体激酶及其基因。 本发明的受体激酶CHRK1具有类似于几丁质酶的细胞外结构域，并且其基因表达受到TMV感染的刺激。 此外，受体激酶CHRK1含有几丁质结合活性以及激酶活性，使其与真菌细胞壁的几丁质结合为几丁质受体，刺激激酶结构域，从而有效地激活通用植物防御系统。 因此，本发明的受体激酶CHRK1可用于开发具有高抗真菌性的植物。

    Method for recombinating plastid using procaryotic recombinase gene
    9.
    发明申请
    Method for recombinating plastid using procaryotic recombinase gene 审中-公开
    使用原核重组酶基因重组质体的方法

    公开(公告)号：US20050204433A1

    公开(公告)日：2005-09-15

    申请号：US10500664

    申请日：2002-12-31

    申请人： Jang-Ryol Liu ,  Won-Joong Jeong ,  Sung-ran Min ,  Seok-won Jbong ,  Su-kyoung Han

    发明人： Jang-Ryol Liu ,  Won-Joong Jeong ,  Sung-ran Min ,  Seok-won Jbong ,  Su-kyoung Han

    IPC分类号： A01H1/00 ,  C12N15/09 ,  C12N15/82

    CPC分类号： C12N15/8214 ,  C12N15/8213

    摘要： The objective of this invention is to enhance the efficiency of plastid transformation using nuclear transformed plants in which the microbial recombinase A(recA) is to target to (or expressed in) the plastid. This invention will be better explained by the following detailed descriptions. A plant is transformed with a nuclear transformation vector containing the microbial recA gene added with a plastid targeting sequence. In this nuclear transformed plant, the frequency of plastid transformation is enhanced greater than two-folds due to increased homologous recombination between the plastid transformation vector carrying genes of interest (or target genes) and the plastid genome. In addition, because plastid transformation is accomplished through a gradual process, adventitious shoots selected after being subjected to plastid transformation should be cut into explants, and then shoots regenerated from the explants are to be reselected until all of the plastids in the shoots are uniformly transformed. However, when the nuclear transformed plant is used, the number of reselection is reduced to ½ to ⅓ due to increased homologous recombination.

    摘要翻译： 本发明的目的是使用核转化植物提高质体转化的效率，其中微生物重组酶A（recA）靶向（或表达于）质体。 以下的详细说明将更好地说明本发明。 用含有质体靶向序列的微生物recA基因的核转化载体转化植物。 在这种核转化植物中，质体转化的频率由于携带感兴趣的基因（或靶基因）的质体转化载体和质体基因组之间的同源重组增加而增加了大于2倍。 另外，由于质体转化是通过逐步过程完成的，因此应将外来物质切割后进入质体转化后的不定芽进行切割，然后重新选择从外植体中再生的枝条，直到枝条中的所有质体均匀转化为止 。 然而，当使用核转化植物时，由于增加的同源重组，重选次数减少到1/2至1/3。