公开(公告)号：US11530424B1

公开(公告)日：2022-12-20

申请号：US16587405

申请日：2019-09-30

申请人： Jia-Wang Wang ,  Richard F. Lockey

发明人： Jia-Wang Wang ,  Richard F. Lockey

IPC分类号： C12N15/90 ,  C12N15/85

摘要： The clustered regularly interspaced short palindromic repeat (CRISPR) gene editing technique, based on the non-homologous end-joining (NHEJ) repair pathway, can efficiently generate gene knockouts of variably sizes. More precise genome editing, either the insertion or deletion of a desired fragment, can be done by combining the homology-directed-repair (HDR) pathway with CRISPR cleavage. HDR-mediated gene knock-in experiments are inefficient, with no reports of successful gene knock-in with DNA fragments larger than 4 kb. Targeted insertion of large DNA fragments (7.4 and 5.8 kb) into the genomes of mouse embryonic stem cells and zygotes, respectively, using the CRISPR/HDR technique without NHEJ inhibitors was performed and indicate that CRISPR/HDR without NHEJ inhibitors can result in highly efficient gene knock-in, equivalent to CRISPR/HDR with NHEJ inhibitors. Although NHEJ is the dominant repair pathway associated with CRISPR-mediated double-strand breaks (DSBs), and biallelic gene knock-ins are common, NHEJ and biallelic gene knock-ins were not detected.

公开(公告)号：US10030263B1

公开(公告)日：2018-07-24

申请号：US14833588

申请日：2015-08-24

申请人： Jia-Wang Wang ,  Richard F. Lockey

发明人： Jia-Wang Wang ,  Richard F. Lockey

IPC分类号： C12Q1/686

摘要： Described herein are multiplexed RNA, including miRNA, PCR-based assays.

公开(公告)号：US20160108108A1

公开(公告)日：2016-04-21

申请号：US14223490

申请日：2014-03-24

申请人： Jia-Wang Wang ,  Richard F. Lockey

发明人： Jia-Wang Wang ,  Richard F. Lockey

IPC分类号： C07K16/18 ,  A61K38/05 ,  C12N15/113 ,  A61K38/17

CPC分类号： C07K16/18 ,  A61K31/69 ,  A61K31/713 ,  A61K35/16 ,  A61K38/1709 ,  A61K45/06 ,  A61K2039/505 ,  C07K14/4702 ,  C07K14/4713 ,  C07K2317/76 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/531 ,  C12N2330/10 ,  C12N2330/50 ,  A61K2300/00

摘要： Anti-cytokine therapy has revolutionized immunological disease treatment, but is not always effective and subject to treatment resistance as the cytokine cascade is highly redundant and multiple cytokines are involved in inflammation. Targeting a critical common regulator of inflammatory effectors is desirable. Lipopolysaccharide (LPS)-responsive beige-like anchor (LRBA) is a master regulator of multiple genes important for inflammation. Subcellular localization shows that LRBA translocated to the nucleus upon LPS stimulation and colocalized with multiple proteins associated with the endosome membrane system, indicating a critical role in membrane/vesicle trafficking essential for deposition, secretion and signal transduction of immune effectors. Deregulation, deficiency, down-regulation and overexpression of LRBA causes defective trafficking and signaling of immune effector molecules, resulting in immunodeficiency and autoimmunity diseases associated with a broader spectrum of severe symptoms when compared to other CVID genes. Modulating LRBA through antibodies, dominant negative mutants, or small interference RNA can be used to treat inflammatory diseases.

摘要翻译： 抗细胞因子治疗已经彻底革新了免疫疾病的治疗，但并不总是有效的，并且受到治疗抗性，因为细胞因子级联是高度冗余的，并且多种细胞因子参与炎症。 靶向炎症反应的关键共同调节剂是可取的。 脂多糖（LPS） - 反应性米色样锚（LRBA）是对炎症重要的多种基因的主要调节剂。 亚细胞定位显示，LPS刺激后，LRBA转移到细胞核，并与多个与内膜膜系统相关的蛋白质共定位，表明在免疫作用子的沉积，分泌和信号传导所必需的膜/囊泡运输中起关键作用。 与其他CVID基因相比，LRBA的放松，缺乏，下调和过表达导致免疫效应分子的不良运输和信号转导，导致与广泛的严重症状相关的免疫缺陷和自身免疫疾病。 通过抗体，显性负突变体或小干扰RNA调节LRBA可用于治疗炎症性疾病。

公开(公告)号：US09264238B2

公开(公告)日：2016-02-16

申请号：US13306548

申请日：2011-11-29

申请人： Lusheng Ji ,  Robert Raymond Miller, II ,  Jia Wang

发明人： Lusheng Ji ,  Robert Raymond Miller, II ,  Jia Wang

IPC分类号： G06F15/173 ,  H04L12/00 ,  H04L12/46 ,  H04L29/00 ,  H04L29/06 ,  H04N21/4363 ,  H04W4/00 ,  H04W84/18

CPC分类号： H04W4/80 ,  H04L12/00 ,  H04L12/4641 ,  H04L29/00 ,  H04L65/1016 ,  H04L65/1046 ,  H04L65/403 ,  H04N21/43637 ,  H04W76/14 ,  H04W84/18

摘要： A system that incorporates teachings of the present disclosure may include, for example, a coordinator device having a memory, and a controller. The memory can have computer instructions, which when executed by the controller, causes the controller to facilitate establishing a first virtual personal area network with a first sensor by executing computer instructions associated with a first application profile, and facilitate establishing a second virtual personal area network with a second sensor by executing computer instructions associated a second application profile. The first application profile can be defined by a first protocol specification, while the second application profile can be defined by a second protocol specification. The first protocol specification can also be operationally distinct from the second protocol specification. Other embodiments are disclosed.

摘要翻译： 结合本公开的教导的系统可以包括例如具有存储器的协调器设备和控制器。 所述存储器可以具有计算机指令，当所述控制器执行时，所述控制器使得所述控制器通过执行与第一应用简档相关联的计算机指令来促进与第一传感器建立第一虚拟个人区域网络，并且促进建立第二虚拟个人区域网络 通过执行与第二应用简档相关联的计算机指令的第二传感器。 第一应用简档可以由第一协议规范定义，而第二应用简档可以由第二协议规范定义。 第一协议规范也可以在操作上与第二协议规范不同。 公开了其他实施例。

公开(公告)号：US08903821B2

公开(公告)日：2014-12-02

申请号：US12462084

申请日：2009-07-29

申请人： Ping Wang ,  Xu Qiang Yue ,  Chen Zhu ,  Gan Wu ,  Wei Wen Hua ,  Jia Wang

发明人： Ping Wang ,  Xu Qiang Yue ,  Chen Zhu ,  Gan Wu ,  Wei Wen Hua ,  Jia Wang

IPC分类号： G06F7/00 ,  G06F17/30 ,  G06Q30/06 ,  G06Q30/02 ,  G06F13/14

CPC分类号： G06F17/3089 ,  G06F17/30864 ,  G06Q30/02 ,  G06Q30/0603 ,  G06Q30/08

摘要： Providing object information on the Internet includes configuring a plurality of front-end categories for a plurality of network objects and a plurality of screening conditions for screening the plurality of network objects, where each of the plurality of screening conditions uniquely corresponding to a respective one of the plurality of the front-end categories. Providing object information further includes receiving object information pertaining to a network object, the object information including a screening condition for the network object; storing the object information in a back-end category, determining a front-end category to which the network object belongs, according to the screening condition and storing correlation information of the object and the front-end category, wherein the back-end category is separate from and different than the front-end category.

摘要翻译： 在互联网上提供对象信息包括配置用于多个网络对象的多个前端类别以及用于筛选多个网络对象的多个筛选条件，其中多个筛选条件中的每一个唯一地对应于 多个前端类别。 提供对象信息还包括接收关于网络对象的对象信息，所述对象信息包括所述网络对象的筛选条件; 将对象信息存储在后端类别中，根据筛选条件确定网络对象所属的前端类别，并存储对象和前端类别的相关信息，其中后端类别为 与前端类别分开且不同。

公开(公告)号：US08826314B2

公开(公告)日：2014-09-02

申请号：US13360828

申请日：2012-01-30

申请人： Jia Wang ,  Andrea Basso ,  Min-Hsuan Chen ,  Zihui Ge ,  Ajay Mahimkar ,  Han Hee Song ,  Jennifer Yates ,  Yin Zhang

发明人： Jia Wang ,  Andrea Basso ,  Min-Hsuan Chen ,  Zihui Ge ,  Ajay Mahimkar ,  Han Hee Song ,  Jennifer Yates ,  Yin Zhang

IPC分类号： H04H60/32

CPC分类号： H04L65/4076 ,  H04L65/103 ,  H04L65/80 ,  H04N21/2402 ,  H04N21/44222 ,  H04N21/64322 ,  H04N21/6583

摘要： A system that incorporates teachings of the present disclosure may include, for example, obtaining regression coefficients that quantify a relationship between premises feedback and first network and premises performance indicators, obtaining second network performance indicators for the network elements, obtaining second premises performance indicators for the customer premises equipment, and predicting customer complaints by applying the obtained regression coefficients to at least the second network performance indicators and the second premises performance indicators. Other embodiments are disclosed.

摘要翻译： 结合本公开的教导的系统可以包括例如获得量化房屋反馈与第一网络和房屋性能指标之间的关系的回归系数，获得网络元件的第二网络性能指标，获得第二房屋性能指标 客户驻地设备，以及通过将获得的回归系数应用于至少第二网络性能指标和第二房屋性能指标来预测客户投诉。 公开了其他实施例。

公开(公告)号：US08813001B2

公开(公告)日：2014-08-19

申请号：US12361845

申请日：2009-01-29

申请人： Hai Zhou ,  Jia Wang

发明人： Hai Zhou ,  Jia Wang

IPC分类号： G06F17/50

CPC分类号： G06F17/5031 ,  G06F17/505 ,  G06F2217/84

摘要： A method for use in electronic design software efficiently and optimally produces minimized or reduced register flip flop area or number of registers/flip flops in a VLSI circuit design without changing circuit timing or functionality. The method dynamically generates constraints; maintains the generated constraints as a regular tree; and incrementally relocates registers/flip flops and/or the number of registers/flip flops in the circuit design.

摘要翻译： 一种用于电子设计软件的方法在VLSI电路设计中有效和最佳地产生最小化或减少的寄存器触发器区域或寄存器/触发器数量，而不改变电路时序或功能。 该方法动态生成约束; 将生成的约束保持为常规树; 并且在电路设计中递增地重新定位寄存器/触发器和/或寄存器/触发器的数量。

公开(公告)号：US08619766B2

公开(公告)日：2013-12-31

申请号：US11681526

申请日：2007-03-02

申请人： Jia Wang ,  Suman Banerjee ,  Qunfeng Dong

发明人： Jia Wang ,  Suman Banerjee ,  Qunfeng Dong

IPC分类号： H04L12/28 ,  H04L12/56 ,  G06F12/00 ,  G06F13/00

CPC分类号： H04L45/00 ,  H04L45/60 ,  H04L47/10 ,  H04L47/2441

摘要： A method and apparatus for classifying packets, e.g., at wire speed are disclosed. The method receives a packet and processes the packet through a hardware-based packet classifier having at least one evolving rule. The method then processes the packet through a software-based packet classifier if the hardware-based packet classifier is unable to classify the packet. In one embodiment, the at least one evolving rule is continuously modified in accordance with learned traffic characteristics of the received packets.

摘要翻译： 公开了一种用于分组的方法和装置，例如以线速度。 该方法接收分组并通过具有至少一个演进规则的基于硬件的分组分类器处理分组。 该方法然后通过基于软件的分组分类器处理分组，如果基于硬件的分组分类器不能对分组进行分类。 在一个实施例中，根据接收到的分组的学习的业务特性来连续地修改至少一个演进规则。

公开(公告)号：US20130045885A1

公开(公告)日：2013-02-21

申请号：US13579316

申请日：2011-02-18

申请人： Shyam S. Mohapatra ,  Jia-Wang Wang

发明人： Shyam S. Mohapatra ,  Jia-Wang Wang

IPC分类号： C40B30/04 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6851 ,  C12N2310/141 ,  C12N2320/10 ,  C12Q2525/301 ,  C12Q2525/173 ,  C12Q2521/107

摘要： The present invention provides materials and methods for detecting, quantifying, and/or profiling microRNAs. Advantageously, the present invention is sensitive, specific, convenient, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of miRNAs, a universal reverse primer for PCR amplification reaction, and a universal probe. In another embodiment, the present invention provides assays that allow the detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction and a single qPCR reaction.

摘要翻译： 本发明提供用于检测，定量和/或分析微小RNA的材料和方法。 有利地，本发明是敏感的，具体的，方便的和具有成本效益的。 在一个实施方案中，本发明提供了用于miRNA逆转录的通用引物，用于PCR扩增反应的通用反向引物和通用探针。 在另一个实施方案中，本发明提供允许使用单次逆转录反应和单次qPCR反应来检测和/或定量多个靶miRNA的测定。

公开(公告)号：US20120246308A1

公开(公告)日：2012-09-27

申请号：US13490683

申请日：2012-06-07

申请人： Alexandre Gerber ,  Jia Wang ,  Oliver Spatscheck ,  Jennifer Yates ,  Michael Merritt

发明人： Alexandre Gerber ,  Jia Wang ,  Oliver Spatscheck ,  Jennifer Yates ,  Michael Merritt

IPC分类号： G06F15/173

CPC分类号： H04L12/66

摘要： A method includes receiving network distance information, receiving a request from a client for an identity of a peer providing content, and identifying a first peer and a second peer providing the content. The network distance information includes a compilation of network distance information provided by a plurality of service providers. The method further includes determining that a network distance between the first peer and the client is less than a network distance between the second peer and the client based on the network distance information, and providing the identity of the first peer to the client.

摘要翻译： 一种方法包括接收网络距离信息，从客户端接收针对提供内容的对等体的身份的请求，以及标识提供内容的第一对等体和第二对等体。 网络距离信息包括由多个服务提供商提供的网络距离信息的汇编。 所述方法还包括基于所述网络距离信息确定所述第一对等端和所述客户端之间的网络距离小于所述第二对等端与所述客户端之间的网络距离，以及将所述第一对等体的身份提供给所述客户端。