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    Methods for producing ligand arrays
    2.
    发明授权
    Methods for producing ligand arrays 失效
    制备配体阵列的方法

    公开(公告)号：US07202358B2

    公开(公告)日：2007-04-10

    申请号：US10206877

    申请日：2002-07-25

    申请人： John S. Hargreaves

    发明人： John S. Hargreaves

    IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C40B40/06 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y30/00 ,  C40B30/04 ,  C40B40/10 ,  C40B50/18 ,  G01N33/54353

    摘要： Methods for producing ligand arrays, e.g., peptide and nucleic acid arrays, as well as the arrays produced thereby, methods for use of the arrays and kits that include the same are provided. In one embodiment of the subject methods, a substrate having a surface displaying acetate functional groups that produce surface bound hydroxyl functional groups upon hydrolysis is first provided, where the acetate groups are then hydrolyzed to hydroxyl functional groups. In another embodiment of the subject methods, a substrate having a surface displaying alkenyl functional groups is first provided, where the alkenyl functional groups are then converted to carboxylic functional groups. The resultant substrates, optionally after an additional functionalization step, are then contacted with ligands, e.g., via deposition of each different ligand onto a different region of the surface, resulting in covalent attachment of the contacted ligands to the surface. The subject methods find use in the preparation of a variety of different types of arrays, where the produced arrays find use in a variety of different applications, including both genomic and proteomic applications.

    摘要翻译： 提供了制备配体阵列的方法，例如肽和核酸阵列，以及由此制备的阵列，使用阵列的方法和包括其的试剂盒。 在本发明方法的一个实施方案中，首先提供具有显示在水解时产生表面结合的羟基官能团的乙酸酯官能团的表面的基材，其中乙酸酯基团然后水解成羟基官能团。 在本方法的另一个实施方案中，首先提供具有显示烯基官能团的表面的基材，其中烯基官能团然后转化为羧基官能团。 任选地在另外的官能化步骤之后，所得到的底物然后与配体接触，例如通过将各种不同配体沉积在表面的不同区域上，导致所接触的配体与表面共价连接。 主题方法用于制备各种不同类型的阵列，其中所产生的阵列可用于各种不同的应用，包括基因组和蛋白质组学应用。

    Methods for generating ligand arrays
    3.
    发明授权
    Methods for generating ligand arrays 失效
    产生配体阵列的方法

    公开(公告)号：US06919181B2

    公开(公告)日：2005-07-19

    申请号：US10106685

    申请日：2002-03-25

    申请人： John S. Hargreaves

    发明人： John S. Hargreaves

    IPC分类号： B05D3/00 ,  C07H21/04 ,  C12M1/34 ,  C12M1/36 ,  C12Q1/68

    CPC分类号： C12Q1/6837 ,  C07H21/04

    摘要： Methods of producing ligand arrays, e.g., polypeptide and nucleic acid arrays, as well as the arrays produced thereby, methods for use of the arrays and kits that include the same, are provided. In the subject methods, a substrate having a surface displaying carboxylic ester functional groups that produce surface bound hydroxyl functional groups upon hydrolysis is first provided. Next, the functional groups are hydrolyzed to hydroxyl functional groups. The resultant substrate, optionally after an additional functionalization step, is then contacted with ligands, e.g., via deposition of each different ligand onto a different region of the surface, resulting in covalent attachment of the contacted ligands to the surface.

    摘要翻译： 提供了制备配体阵列的方法，例如多肽和核酸阵列，以及由此产生的阵列，使用阵列的方法和包括其的试剂盒。 在本发明方法中，首先提供具有表现出在水解时产生表面结合的羟基官能团的羧酸酯官能团的表面的基材。 接下来，将官能团水解成羟基官能团。 然后任选地在另外的官能化步骤之后，将所得底物与配体接触，例如通过将各种不同配体沉积在表面的不同区域上，导致所接触的配体与表面的共价连接。

    Organic electroluminescent device
    4.
    发明授权
    Organic electroluminescent device 有权
    有机电致发光器件

    公开(公告)号：US06552488B1

    公开(公告)日：2003-04-22

    申请号：US09382025

    申请日：1999-08-24

    申请人： Daniel B. Roitman ,  John S. Hargreaves ,  Homer Antoniadis ,  Karen L. Seaward

    发明人： Daniel B. Roitman ,  John S. Hargreaves ,  Homer Antoniadis ,  Karen L. Seaward

    IPC分类号： H05B3306

    CPC分类号： H01J37/32082 ,  H01L27/322 ,  H01L27/3244 ,  H01L51/5253 ,  H01L51/529 ,  H01L2251/5315

    摘要： A color organic electroluminescent device includes color filters, light emitting layers and substrate integrated into a single assembly, with the light emitting layers lying between the substrate and the color filters. Unlike color electroluminescent devices where an image must be viewed through a transparent substrate, or where color filters are deposited upon another device and later combined with the assembly, the present disclosure calls for a transparent protective layer of silicon nitride or a similar material to be deposited over the light emitting layers to protect the light emitting layers from oxygen and moisture, and to serve as a platform for color filters. In order to achieve sufficient density in the protective layer and avoid defects, a cold plasma deposition or similar process is used for deposition of the protective layer. By viewing light through this protective layer instead of a substrate, nearly any substrate can be used.

    摘要翻译： 彩色有机电致发光器件包括滤色器，发光层和集成到单个组件中的衬底，其中发光层位于衬底和滤色器之间。 不同于彩色电致发光器件，其中必须通过透明衬底观察图像，或者滤色器沉积在另一器件上并且随后与组件结合在一起，本公开要求具有氮化硅的透明保护层或要沉积的类似材料 在发光层上，以保护发光层免受氧气和水分的影响，并且用作滤色器的平台。 为了在保护层中获得足够的密度并避免缺陷，使用冷等离子体沉积或类似的工艺来沉积保护层。 通过观察通过该保护层的光而不是基板，可以使用几乎任何基板。

    Method of polynucleotide synthesis using modified support
    5.
    发明授权
    Method of polynucleotide synthesis using modified support 失效
    使用改良载体的多核苷酸合成方法

    公开(公告)号：US07585970B2

    公开(公告)日：2009-09-08

    申请号：US10652049

    申请日：2003-08-30

    申请人： Douglas J Dellinger ,  Geraldine F Dellinger ,  John S Hargreaves

    发明人： Douglas J Dellinger ,  Geraldine F Dellinger ,  John S Hargreaves

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00 ,  Y02P20/55

    摘要： A method for synthesizing a polynucleotide is disclosed. The method comprises providing a functionalized support comprising a triaryl methyl linker bound to the solid support through a substitution on one of the aryl groups, a nucleotide chain bound to the central methyl carbon of the triaryl methyl group and a nucleoside with a reactive site hydroxyl. The method further provides contacting that functionalized support with a precursor having a hydroxyl protecting group and a phosphorous derivative reactive group to produce internucleotide linkage. The resulting compound is then treated to both remove the hydroxyl protecting group on the precursor, and to oxidize the internucleotide bond.

    摘要翻译： 公开了一种合成多核苷酸的方法。 该方法包括提供官能化载体，其包含通过在一个芳基上取代而与固体支持物结合的三芳基甲基接头，与三芳基甲基的中心甲基结合的核苷酸链和具有反应性位点羟基的核苷。 该方法还使官能化载体与具有羟基保护基团和磷衍生物反应性基团的前体接触以产生核苷酸间键合。 然后处理所得化合物以去除前体上的羟基保护基团并氧化核苷酸间键。

    Functionalization of substrate surfaces with silane mixtures
    7.
    发明授权
    Functionalization of substrate surfaces with silane mixtures 有权
    用硅烷混合物对基体表面进行功能化

    公开(公告)号：US06660338B1

    公开(公告)日：2003-12-09

    申请号：US09802653

    申请日：2001-03-08

    申请人： John S. Hargreaves

    发明人： John S. Hargreaves

    IPC分类号： B05D500

    CPC分类号： C03C17/30 ,  B05D1/18 ,  B05D5/08 ,  C03C17/3405

    摘要： Low surface energy functionalized surfaces on solid supports are provided by contacting a surface of a solid substrate having reactive moieties on the surface with a solution of an organic solvent and water. The surface of the solid substrate is then contacted with a derivatizing composition containing a mixture of silanes. A first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest, such as small molecules. Molecular moieties of interest may be attached through cleavable sites. Derivatizing compositions for carrying out the surface functionalization process are provided as well.

    摘要翻译： 通过将表面上具有反应性部分的固体基材的表面与有机溶剂和水的溶液接触来提供固体载体上的低表面能官能化表面。 然后将固体基质的表面与含有硅烷混合物的衍生化组合物接触。 第一硅烷提供所需的表面能降低，而第二硅烷能够与目标分子部分如小分子进行功能化。 感兴趣的分子部分可以通过可裂解位点附着。 还提供用于进行表面官能化过程的衍生组合物。