公开(公告)号：US20080174766A1

公开(公告)日：2008-07-24

申请号：US11915316

申请日：2006-05-24

Applicant: Ville Haaslahti ,  Juhani Aalto ,  Timo Oikari ,  Tero Soukka

Inventor： Ville Haaslahti ,  Juhani Aalto ,  Timo Oikari ,  Tero Soukka

IPC: G01N21/64

CPC classification number: G01N33/542 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/582

Abstract: This invention relates to a method to correct for error in an anti-Stokes photoluminescence measurement in a Foerster-type resonance energy-transfer assay. The method comprises the steps of exciting anti-Stokes photoluminescent molecules, ions, phosphors, chelates, or particles, i.e. donors of the assay, with one wavelength or multiple wavelengths of light wherein the wavelength or wavelengths are greater than that of an emission wavelength of acceptor molecules in the assay; measuring emission light signal at a wavelength, which is the emission wavelength of the acceptor molecules in the Foerster-type resonance energy-transfer assay, and which differs from the emission wavelength of the anti-Stokes photoluminescent donor in at least two different time windows; a first time window within the time window defined by the excitation light pulse, i.e. within the time window opening when the excitation light pulse is switched on and closing when the excitation light pulse is switched off, and a second time window to follow the first time window not at all overlapping with the time window defined by the excitation pulse; and correcting the emission light signal measured, comprising signals originating from non-radiative and radiative energy transfer, within the first time window by estimating the ratio of the signals from non-radiative and radiative energy transfer or the signal originating from radiative energy transfer using at least one emission light signal measured in the second time window. This invention also relates to the use of a device according to the method of the invention, a system for carrying out the method and a software product for the system.

Abstract translation: 本发明涉及一种在Foerster型共振能量转移测定法中校正反斯托克斯光致发光测量中的误差的方法。 该方法包括以一波长或多波长的光激发抗斯托克斯光致发光分子，离子，磷光体，螯合物或颗粒，即测定的供体的步骤，其中波长或波长大于发射波长的波长或波长 受体分子在测定中; 在Foerster型共振能量转移测定中测量作为受体分子的发射波长的波长的发射光信号，并且其在至少两个不同时间窗中与反斯托克斯光致发光供体的发射波长不同; 即由激发光脉冲定义的时间窗内的第一时间窗口，即在激发光脉冲被接通时关闭的时间窗口内，当关闭激发光脉冲时关闭的第一时间窗口，以及第一时间窗口 窗口与激励脉冲定义的时间窗口完全不重叠; 以及在第一时间窗内通过估计来自非辐射能量和辐射能量传递的信号的比率或源自辐射能量传输的信号的比率来校正在第一时间窗内测量的包括源自非辐射和辐射能量传递的信号的发射光信号 在第二时间窗中测量的至少一个发射光信号。 本发明还涉及根据本发明的方法的设备的使用，用于执行该方法的系统和用于该系统的软件产品。

公开(公告)号：US5185525A

公开(公告)日：1993-02-09

申请号：US761942

申请日：1991-09-23

Applicant: Juhani Aalto ,  Seppo Wallenius

Inventor： Juhani Aalto ,  Seppo Wallenius

IPC: G01T7/08

CPC classification number: G01T7/08

Abstract: The invention relates to a changer mechanism for individual measurement of radioactive samples in a gamma counter. The changer mechanism comprises an identifying element for identifying sample containers of different sizes and a transferring device for transferring the sample containers to the measuring unit for measuring, and back therefrom after measuring. According to the invention, the changer mechanism comprises a lifting device having a grip unit for lifting and transferring the sample from the transfer unit to the measuring unit. The grip unit is provided with grip elements for transferring samples of different sizes based on the information obtained from the identifying unit.

Abstract translation: PCT No.PCT / FI90 / 00070 Sec。 371日期1991年9月23日 102（e）1991年9月23日PCT PCT 1990年3月21日PCT公布。 出版物WO90 / 11535 日期1990年10月4日。本发明涉及一种用于伽马计数器中放射性样品单独测量的更换机构。 更换器机构包括用于识别不同尺寸的样品容器的识别元件和用于将样品容器传送到测量单元进行测量并在测量之后从其返回的转移装置。 根据本发明，更换器机构包括具有用于将样品从传送单元提升并传送到测量单元的把手单元的提升装置。 把手单元设置有用于基于从识别单元获得的信息传送不同尺寸的样本的把手元件。

公开(公告)号：US08479758B2

公开(公告)日：2013-07-09

申请号：US12716910

申请日：2010-03-03

Applicant: Juhani Aalto

Inventor： Juhani Aalto

IPC: F17D1/00

CPC classification number: E03B11/12 ,  E03B7/02 ,  E03B7/075 ,  Y02A20/104 ,  Y10T137/0379 ,  Y10T137/7759 ,  Y10T137/7761 ,  Y10T137/85938 ,  Y10T137/87877 ,  Y10T137/87917

Abstract: New methods and devices for reducing water consumption by restricting the supply pressures at the water consumption points.

Abstract translation: 通过限制用水量供应压力来减少用水量的新方法和装置。

公开(公告)号：US07826052B2

公开(公告)日：2010-11-02

申请号：US11915316

申请日：2006-05-24

Applicant: Ville Haaslahti ,  Juhani Aalto ,  Timo Oikari ,  Tero Soukka

Inventor： Ville Haaslahti ,  Juhani Aalto ,  Timo Oikari ,  Tero Soukka

IPC: G01N21/64

CPC classification number: G01N33/542 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/582

Abstract: A method to correct measurement error in a resonance energy-transfer assay, including exciting anti-Stokes photoluminescent donors with at least one wavelength of light which is greater than an emission wavelength of acceptor molecules; measuring emission at the acceptor's emission wavelength and which differs from the donor's emission wavelength in at least two different time windows; a first time window within the time window defined by the excitation light pulse and a second non-overlapping time window which follows the first time window; and correcting the emission signal, which includes signals originating from non-radiative and radiative energy transfer, within the first time window by estimating the ratio of the signals from non-radiative and radiative energy transfer or the signal originating from radiative energy transfer using at least one emission signal measured in the second time window.

Abstract translation: 一种用于校正共振能量转移测定中的测量误差的方法，包括激发具有至少一个波长的光的反斯托克斯光致发光供体，其大于受主分子的发射波长; 在受体发射波长处测量发射，并且在至少两个不同的时间窗中与供体的发射波长不同; 由激励光脉冲定义的时间窗内的第一时间窗口和跟随第一时间窗的第二非重叠时间窗; 以及在第一时间窗口内通过估计来自非辐射能量和辐射能量传递的信号的比率或者来自辐射能量传输的信号，至少校正包括来自非辐射能量和辐射能量传递的信号的发射信号 在第二时间窗中测量的一个发射信号。

公开(公告)号：US20060127279A1

公开(公告)日：2006-06-15

申请号：US10522796

申请日：2003-07-30

Applicant: Juhani Aalto ,  Jukka Haaslahti

Inventor： Juhani Aalto ,  Jukka Haaslahti

IPC: G01N21/64

CPC classification number: G01N21/6452

Abstract: The object of the invention is a multifunction instrument (10 ) for measuring biochemical and medical samples, which are preferably placed into the wells (13) of the sample plates (12) and measured by a detector (20). The detector is movable with slide (26, 27) and guide (22, 23) elements so that a rotating device (14) can turn the detector into vertical position for liquid scintillation counting and/or luminescence counting. The second horizontal position and light path (30) is provided for fluorescence measuring with a light source (40) for excitation. Also an absorbance detector (50) is included.

Abstract translation: 本发明的目的是用于测量生化和医用样品的多功能仪器（10），其优选地放置在样品板（12）的孔（13）中并由检测器（20）测量。 检测器可用滑动件（26,27）和引导件（22,23）元件移动，使得旋转装置（14）可以将检测器转到垂直位置用于液体闪烁计数和/或发光计数。 提供第二水平位置和光路（30）用于用于激发的光源（40）进行荧光测量。 还包括吸光度检测器（50）。

公开(公告)号：US5194733A

公开(公告)日：1993-03-16

申请号：US761941

申请日：1991-09-23

Applicant: Juhani Aalto

Inventor： Juhani Aalto

IPC: G01T7/08

CPC classification number: G01T7/08

Abstract: A multipurpose gamma counter enabling measurement of one sample at a time or alternatively two or more samples at a time and a method for sample handling in gamma measuring. The gamma counter is characterized in that the measuring unit and/or the grip unit for the sample containers are fully or partly formed of replaceable modules so that one or more necessary modular units can be fitted on the gamma counter depending on the measuring method in use at each time. The method is characterized in that, if the samples are research samples, they are measured one at a time in the detector of the research measuring chamber module and, if the samples are RIA samples, they are measured in the first place at least two at a time in the detector of the RIA measuring chamber module.

Abstract translation: PCT No.PCT / FI90 / 00069 Sec。 371日期1991年9月23日 102（e）1991年9月23日PCT PCT 1990年3月21日PCT公布。 出版物WO90 / 11534 1990年10月4日的日期。一种多用途伽马计数器，可一次测量一个样品，或者一次测量两个或多个样品，以及伽马测量中的样品处理方法。 伽马计数器的特征在于，用于样品容器的测量单元和/或夹持单元由可更换的模块完全或部分地形成，使得可以根据使用中的测量方法将一个或多个必需的模块单元安装在伽马计数器上 每次 该方法的特征在于，如果样品是研究样品，则在研究测量室模块的检测器中一次测量一个样品，并且如果样品是RIA样品，则首先测量至少两个 在RIA测量室模块的检测器中的时间。

公开(公告)号：US20100224259A1

公开(公告)日：2010-09-09

申请号：US12716910

申请日：2010-03-03

Applicant: Juhani AALTO

Inventor： Juhani AALTO

IPC: E03B1/00

CPC classification number: E03B11/12 ,  E03B7/02 ,  E03B7/075 ,  Y02A20/104 ,  Y10T137/0379 ,  Y10T137/7759 ,  Y10T137/7761 ,  Y10T137/85938 ,  Y10T137/87877 ,  Y10T137/87917

Abstract: New methods and devices for reducing water consumption by restricting the supply pressures at the water consumption points.

Abstract translation: 通过限制用水量供应压力来减少用水量的新方法和装置。

公开(公告)号：US5268574A

公开(公告)日：1993-12-07

申请号：US761943

申请日：1991-09-23

Applicant: Juhani Aalto ,  Olli Hakala

Inventor： Juhani Aalto ,  Olli Hakala

IPC: G01T7/00 ,  G01T7/08 ,  G01T7/02

CPC classification number: G01T7/00 ,  G01T7/08

Abstract: A transfer device for cassettes containing radioactive samples and a cassette system in a gamma counter provided with a well-type detector. The transfer device comprises a path (61), by which cassettes (11, 12, 13) can be moved one at a time to a lifting position where sample containers can be lifted and moved to the measuring unit (50) for measuring. At the lifting position a vertical long side of the cassette (11, 12, 13) is locked in place against a vertical surface or edge (63) of the table (61) so that the distance between the center of the sample container (17) in the cassette from the vertical surface or edge of the table is substantially directly proportional to the radius of the sample container. The cassette system comprises at least two different cassettes (11, 12, 13) which are of the same length but which can take a different number of adjacently placed sample containers (17 ) of different sizes. The sample containers in each cassette are the same size. The width of the cassette is directly proportional to the width of the sample containers placed therein. The cassettes are also symmetrical about their longitudinal axis so that the center line of the sample containers is coincident with the center line of the cassette. The two long sides of each cassette are nearly similar and vertical.

Abstract translation: PCT No.PCT / FI90 / 00071 Sec。 371日期1991年9月23日 102（e）1991年9月23日PCT PCT 1990年3月21日PCT公布。 出版物WO90 / 11536 日期为1990年10月4日。一种用于带有放射性样品的盒的转移装置和在具有井型检测器的γ计数器中的盒系统。 传送装置包括路径（61），盒子（11,12,13）可以一次一个地移动到提起位置，在该提升位置，样品容器可被提起并移动到测量单元（50）进行测量。 在提升位置，盒（11,12,13）的垂直长边被锁定在桌子（61）的垂直表面或边缘（63）上，使得样品容器（17）的中心之间的距离 ）从桌子的垂直表面或边缘开始大致与样品容器的半径成正比。 盒式系统包括至少两个长度相同但不同数量的不同尺寸的相邻放置的样品容器（17）的不同盒（11,12,13）。 每个盒中的样品容器尺寸相同。 盒的宽度与放置在其中的样品容器的宽度成正比。 盒也关于它们的纵向轴线对称，使得样品容器的中心线与盒的中心线重合。 每个盒子的两个长边几乎相似和垂直。