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    Grapevine leafroll virus (type 2) proteins and their uses
    1.
    发明授权
    Grapevine leafroll virus (type 2) proteins and their uses 失效
    葡萄卷叶病毒（2型）蛋白质及其用途

    公开(公告)号：US06858426B1

    公开(公告)日：2005-02-22

    申请号：US09613486

    申请日：2000-07-11

    申请人： Hai-Ying Zhu ,  Kai-Shu Ling ,  Dennis Gonsalves

    发明人： Hai-Ying Zhu ,  Kai-Shu Ling ,  Dennis Gonsalves

    IPC分类号： C07K14/08 ,  C12N15/33 ,  C12N15/40 ,  C12N15/82 ,  C12Q1/70 ,  G01N33/569 ,  C12N15/00 ,  C07H21/02 ,  C12N1/20 ,  C12N5/00 ,  C12P21/06

    CPC分类号： C07K14/005 ,  C12N15/8283 ,  C12N2770/00022 ,  C12Q1/70 ,  G01N33/56961 ,  G01N33/56983 ,  G01N2333/08

    摘要： The present invention relates to isolated proteins or polypeptides of grapevine leafroll virus (type 2). The encoding DNA molecules either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant are also disclosed. Other aspects of the present invention relates to a method of imparting grapevine leafroll resistance, to grape and tobacco plants by transforming them with the DNA molecules of the present invention, a method of imparting beet yellows virus resistance to a beet plant, a method of imparting tristeza virus resistance to a citrus plant, and a method of detecting the presence of a grapevine leafroll virus, such as GRLaV-2, in a sample.

    摘要翻译： 本发明涉及葡萄卷叶病毒的分离蛋白质或多肽（2型）。 也公开了分离形式或表达系统中的单独编码DNA分子，宿主细胞或转基因葡萄植物。 本发明的其他方面涉及通过用本发明的DNA分子转化葡萄和烟草植物来赋予葡萄和卷烟抗性的方法，赋予甜菜植物甜菜黄病毒抗性的方法，赋予甜菜甜菜植物的方法 对柑橘类植物的病毒抗性，以及检测样品中葡萄卷叶病毒如GRLaV-2的存在的方法。

    Grapevine leafroll virus proteins and their uses
    4.
    发明申请
    Grapevine leafroll virus proteins and their uses 审中-公开
    葡萄卷叶病毒蛋白及其用途

    公开(公告)号：US20050160492A1

    公开(公告)日：2005-07-21

    申请号：US11010775

    申请日：2004-12-13

    申请人： Dennis Gonsalves ,  Kai-Shu Ling

    发明人： Dennis Gonsalves ,  Kai-Shu Ling

    IPC分类号： C12N9/10 ,  C12N9/50 ,  C12N9/90 ,  C12N15/54 ,  C12N15/82 ,  A01H1/00 ,  C07H21/02 ,  C07K14/08

    CPC分类号： C12N9/503 ,  C12N9/1007 ,  C12N9/90 ,  C12N15/8283

    摘要： The present invention relates to an isolated GLRaV-3 protein or polypeptide selected from a group of a polyprotein, a proteinase, a methyltransferase, a helicase, and an RNA-dependent RNA polymerase. The encoding DNA molecule either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant is also disclosed. Another aspect of the present invention relates to a method of imparting grapevine leafroll resistance to grape plants by transforming them with the DNA molecule of the present invention.

    摘要翻译： 本发明涉及分离的GLRaV-3蛋白或多肽，其选自多蛋白，蛋白酶，甲基转移酶，解旋酶和RNA依赖性RNA聚合酶。 也公开了分离形式或表达系统中的编码DNA分子，宿主细胞或转基因葡萄植物。 本发明的另一方面涉及通过用本发明的DNA分子转化葡萄植物来赋予葡萄植物抗性的方法。

    Grapevine fanleaf virus resistance in grapevine expressing grapevine fanleaf virus coat protein
    5.
    发明授权
    Grapevine fanleaf virus resistance in grapevine expressing grapevine fanleaf virus coat protein 失效
    葡萄叶扇叶病毒外壳蛋白中葡萄叶扇叶病毒抗性

    公开(公告)号：US06667426B1

    公开(公告)日：2003-12-23

    申请号：US09162017

    申请日：1998-09-28

    申请人： Dennis Gonsalves ,  Baodi Xue ,  Tania Krastanova ,  Kai-Shu Ling ,  Marc Fuchs

    发明人： Dennis Gonsalves ,  Baodi Xue ,  Tania Krastanova ,  Kai-Shu Ling ,  Marc Fuchs

    IPC分类号： C12N504

    CPC分类号： C07K14/005 ,  A01H1/04 ,  A01H5/08 ,  A01H6/88 ,  C12N15/8205 ,  C12N15/8283 ,  C12N2770/00022 ,  C12N2770/32022

    摘要： In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a fanleaf disease, the method including the steps of: (a) transforming a grape plant cell with a grape nepovirus coat protein nucleic acid molecule or fragment thereof which is capable of being expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component which expresses, at a low level, the nucleic acid molecule or fragment thereof, wherein the low level expression increases the resistance of the transgenic grapevine or grapevine component to fanleaf disease. The invention also relates to an isolated nucleic acid molecule encoding a coat protein or fragment thereof of a ‘Geneva’ isolate of a grapevine nepovirus virus.

    摘要翻译： 通常，本发明的特征在于一种选择具有增加的扇叶病抗性的转基因葡萄树或葡萄树成分的方法，所述方法包括以下步骤：（a）用葡萄树突状病毒外壳蛋白核酸分子或片段转化葡萄植物细胞 其能够在植物细胞中表达; （b）从植物细胞再生转基因葡萄或葡萄成分; 和（c）选择在低水平表达核酸分子或其片段的转基因葡萄或葡萄成分，其中低水平表达增加转基因葡萄或葡萄成分对扇叶病的抗性。 本发明还涉及编码葡萄树病毒病毒的“日内瓦”分离物的外壳蛋白或其片段的分离的核酸分子。

    Grapevine leafroll virus (type 2) proteins and their uses
    6.
    发明授权
    Grapevine leafroll virus (type 2) proteins and their uses 失效
    葡萄卷叶病毒（2型）蛋白质及其用途

    公开(公告)号：US06197948B1

    公开(公告)日：2001-03-06

    申请号：US09080983

    申请日：1998-05-19

    申请人： Hai-Ying Zhu ,  Kai-Shu Ling ,  Dennis Gonsalves

    发明人： Hai-Ying Zhu ,  Kai-Shu Ling ,  Dennis Gonsalves

    IPC分类号： C12N1533

    CPC分类号： C07K14/005 ,  C12N15/8283 ,  C12N2770/00022 ,  C12Q1/70 ,  G01N33/56961 ,  G01N33/56983 ,  G01N2333/08

    摘要： The present invention relates to isolated proteins or polypeptides of grapevine leafroll virus (type 2). The encoding DNA molecules either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant are also disclosed. Other aspects of the present invention relates to a method of imparting grapevine leafroll resistance to grape and tobacco plants by transforming them with the DNA molecules of the present invention, a method of imparting beet yellows virus resistance to a beet plant, a method of imparting tristeza virus resistance to a citrus plant, and a method of detecting the presence of a grapevine leafroll virus, such as GRLaV-2, in a sample.

    摘要翻译： 本发明涉及葡萄卷叶病毒的分离蛋白质或多肽（2型）。 也公开了分离形式或表达系统中的单独编码DNA分子，宿主细胞或转基因葡萄植物。 本发明的其他方面涉及通过用本发明的DNA分子转化葡萄和烟草植物来赋予葡萄和卷烟抗性的方法，赋予甜菜植物甜菜黄病毒抗性的方法，赋予甜菜甜菜植物的方法 对柑橘类植物的病毒抗性，以及检测样品中葡萄卷叶病毒如GRLaV-2的存在的方法。

    Grapevine fanleaf virus resistance in grapevine
    7.
    发明授权
    Grapevine fanleaf virus resistance in grapevine 有权
    葡萄柚叶病毒抗性

    公开(公告)号：US07211710B2

    公开(公告)日：2007-05-01

    申请号：US10377435

    申请日：2003-02-28

    申请人： Dennis Gonsalves ,  Baodi Xue ,  Tania Krastanova ,  Kai-Shu Ling ,  Marc Fuchs

    发明人： Dennis Gonsalves ,  Baodi Xue ,  Tania Krastanova ,  Kai-Shu Ling ,  Marc Fuchs

    IPC分类号： C12N15/82 ,  C12N15/90

    CPC分类号： C07K14/005 ,  A01H1/04 ,  A01H5/08 ,  A01H6/88 ,  C12N15/8205 ,  C12N15/8283 ,  C12N2770/00022 ,  C12N2770/32022

    摘要： In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a grapevine fanleaf disease. The method involves (a) transforming a grape plant cell with a grapevine fanleaf virus coat protein nucleic acid molecule or fragment thereof which is expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component expressing a grapevine fanleaf virus coat protein or coat protein fragment thereof, where the coat protein or the coat protein fragment thereof is expressed at a level equal to or less than an optical density of about 0.2 at 405 nm as detected by an enzyme-linked immunoassay as compared to a control plant, the selected transgenic grapevine or grapevine component having resistance to the grapevine fanleaf disease.

    摘要翻译： 通常，本发明的特征在于选择具有增加的对葡萄叶扇叶病抗性的转基因葡萄树或葡萄树成分的方法。 该方法包括：（a）在植物细胞中表达的葡萄籽叶病毒外壳蛋白核酸分子或其片段转化葡萄植物细胞; （b）从植物细胞再生转基因葡萄或葡萄成分; 和（c）选择表达葡萄叶扇叶病毒外壳蛋白或外壳蛋白片段的转基因葡萄或葡萄成分，其中外壳蛋白或其外壳蛋白片段以等于或小于约0.2的光密度的水平表达 在与对照植物相比通过酶联免疫测定法检测的405nm处，所选择的转基因葡萄树或葡萄树成分具有对葡萄叶扇叶病的抗性。

    Grapevine leafroll virus proteins and their uses
    10.
    发明授权
    Grapevine leafroll virus proteins and their uses 失效
    葡萄卷叶病毒蛋白及其用途

    公开(公告)号：US06558953B1

    公开(公告)日：2003-05-06

    申请号：US09579259

    申请日：2000-05-25

    申请人： Dennis Gonsalves ,  Kai-Shu Ling

    发明人： Dennis Gonsalves ,  Kai-Shu Ling

    IPC分类号： C12N514

    CPC分类号： C12N9/127 ,  A01H5/08 ,  A01H6/88 ,  C07K14/005 ,  C12N9/90 ,  C12N15/8283 ,  C12N2770/00022

    摘要： The present invention relates to an isolated protein or polypeptide corresponding to a coat protein or polypeptide of a grapevine leafroll virus. The encoding DNA molecule either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant is also disclosed. Another aspect of the present invention relates to a method of imparting grapevine leafroll resistance to grape plants by transforming them with the DNA molecule of the present invention. A method for imparting tristeza virus resistance in citrus plants using the DNA molecule of the present invention is also disclosed.

    摘要翻译： 本发明涉及对应于葡萄卷叶病毒的外壳蛋白或多肽的分离的蛋白质或多肽。 也公开了分离形式或表达系统中的编码DNA分子，宿主细胞或转基因葡萄植物。 本发明的另一方面涉及通过用本发明的DNA分子转化葡萄植物来赋予葡萄植物抗性的方法。 还公开了使用本发明的DNA分子在柑橘类植物中赋予三叉戟病毒抗性的方法。