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    Cell measuring and sorting chip
    1.
    发明授权
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US08361412B2

    公开(公告)日：2013-01-29

    申请号：US11317004

    申请日：2005-12-27

    申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC分类号： B01L3/00

    CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    摘要： A cell sorting device using an inexpensive chip capable of being exchanged for each sample. The chip includes: a first flow path allowing buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow buffer fluid not containing cells to flow down; a fourth flow path which allows the buffer fluid as a single flow path formed by joining the buffer fluids in the other three flow paths; a cell detecting region for detecting cells flowing with the buffer fluid down the fourth flow path; and a cell sorting region for sorting the cells according to a type of the cells detected. The first to fourth flow paths are cascaded, are supplied with the buffer fluid from reservoirs with the same fluid level, and have substantially the same width or cross-section area.

    摘要翻译： 一种使用廉价芯片的细胞分选装置，能够对每个样品进行交换。 芯片包括：允许包含电池的缓冲流体向下流动的第一流动路径; 第二和第三流动路径，其中第一流动路径在其间并且允许不包含电池的缓冲流体向下流动; 第四流路，其允许缓冲流体作为通过连接其它三个流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测沿着第四流动路径流动的缓冲液的细胞; 以及用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一至第四流动路径级联，从具有相同流体水平的储存器提供缓冲流体，并且具有基本上相同的宽度或横截面面积。

    Cell measuring and sorting chip
    4.
    发明申请
    Cell measuring and sorting chip 有权
    电池测量和分选芯片

    公开(公告)号：US20060194307A1

    公开(公告)日：2006-08-31

    申请号：US11317004

    申请日：2005-12-27

    申请人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    发明人： Kenji Yasuda ,  Akihiro Hattori ,  Kazunori Okano

    IPC分类号： C12M1/34 ,  B07C5/00

    CPC分类号： G01N15/147 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12M47/04 ,  G01N35/085 ,  G01N2015/149 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30024

    摘要： The present invention provides a cell sorting device using an inexpensive chip capable of being exchanged for each sample for cell sorting. The cell sorting device uses flow paths formed on the substrate and has the following structure. The chip includes: a first flow path allowing a buffer fluid containing cells to flow down; second and third flow paths which put the first flow path therebetween and allow a buffer fluid not containing cells to flow down from both sides of the first flow path; a fourth flow path which allows the buffer fluid as a single flow path formed by the join of the buffer fluid in the first flow path and the buffer fluid in the second and third flow paths; a cell detecting region for detecting cells flowing with a buffer fluid down the fourth flow path; a cell sorting region for sorting the cells according to a type of the cells detected. The first flow path, the second and the third flow paths, the fourth flow path, the cell detecting region and the cell sorting region provided in a cascade. Two flow paths are provided to allow the cells sorted to flow downstream of the cell sorting region. The buffer fluid flowing in the first to the fourth flow paths is supplied from reservoirs having the same fluid level. The first flow path and the fourth flow path are structured to have substantially the same width or cross-section area.

    摘要翻译： 本发明提供一种使用廉价的芯片的细胞分选装置，其能够用于每个样品进行细胞分选。 细胞分选装置使用形成在基板上的流路，具有以下结构。 芯片包括：允许包含电池的缓冲流体向下流动的第一流路; 第二和第三流动路径，其将第一流动路径放置在其间并允许不含有电池的缓冲流体从第一流动路径的两侧向下流动; 第四流动路径，其允许缓冲流体作为由第一流动路径中的缓冲流体的连接和第二和第三流动路径中的缓冲流体形成的单个流动路径; 细胞检测区域，用于检测在第四流路下方以缓冲液流动的细胞; 用于根据检测到的细胞的类型对细胞进行分选的细胞分选区域。 第一流路，第二流路，第三流路，第四流路，单元检测区域和电池排列区域。 提供了两个流动路径以允许排序的细胞流动到细胞分选区域的下游。 在第一至第四流动路径中流动的缓冲流体从具有相同流体液位的储存器供应。 第一流路和第四流路被构造成具有基本相同的宽度或横截面面积。

    Chemical reaction device, chemical reaction system and chemical reaction method
    5.
    发明申请
    Chemical reaction device, chemical reaction system and chemical reaction method 失效
    化学反应装置，化学反应体系和化学反应方法

    公开(公告)号：US20050014246A1

    公开(公告)日：2005-01-20

    申请号：US10854018

    申请日：2004-05-26

    申请人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    发明人： Yoshinobu Kohara ,  Kazunori Okano ,  Hideyuki Noda

    IPC分类号： G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  G01N33/543 ,  G01N37/00 ,  C12M1/34 ,  G01N33/558

    CPC分类号： G01N33/54366

    摘要： A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel. In a typical reaction vessel having an enzyme immobilized on an interior surface of a capillary and glass beads functioning as an obstacle for the flow filled in the channel of the capillary, a reaction solution can move either in one direction or back and forth in two directions to thereby undergo a reaction with the enzyme immobilized on the interior surface. The flow of the reaction solution is not a laminar flow so that a reaction between the particular molecule immobilized and the reaction solution proceeds at high efficiency.

    摘要翻译： 提供化学反应装置用于固定在固相上的分子和溶液中的分子之间的化学反应，并且还提供化学分析装置，以通过化学反应和随后的固定在固相上的分子捕获溶液中的分子 捕获的分子的测量。 从而提高了反应效率以及样品通量。 化学反应装置和化学分析装置使用用于反应容器的微流体装置的通道，并且至少一个特定的分子固定在内表面上，并且固定结构或非固定的阻碍流动的障碍物 渠道。 在具有固定在毛细管的内表面上的酶的典型的反应容器和作为填充在毛细管的通道中的流动的障碍物的玻璃珠上，反应溶液可以在一个方向或两个方向上来回移动 从而与固定在内表面上的酶进行反应。 反应溶液的流动不是层流，使得固定的特定分子与反应溶液之间的反应以高效率进行。

    DNA probe array
    7.
    发明授权
    DNA probe array 有权
    DNA探针阵列

    公开(公告)号：US06391562B2

    公开(公告)日：2002-05-21

    申请号：US09808949

    申请日：2001-03-16

    申请人： Hideki Kambara ,  Kazunori Okano

    发明人： Hideki Kambara ,  Kazunori Okano

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00657 ,  B01J2219/00702 ,  B01J2219/00707 ,  B01J2219/00722 ,  C40B40/06 ,  Y10S436/805 ,  Y10S436/809

    摘要： There is used at least one probe array obtained by arraying particles having various probes, respectively, fixed thereon (probe particles) in a definite order in a holder. A plurality of capillaries or grooves packed with various kinds, respectively, of probe particles are arrayed in parallel, and one of particles contained in each capillary or groove is injected into another capillary or groove to produce a probe array in which the various kinds of probe particles are arrayed in a constant and definite order. Various fluorophore-labeled DNA's are measured at the same time by attaching various probes to particles, respectively, of different sizes. A probe array composed of various fixed DNA probes can easily be produced, and there can be provided a probe array for detecting various DNA's which is composed of various fixed arbitrary DNA probes.

    摘要翻译： 使用至少一个探针阵列，其通过将具有各种探针的颗粒分别在固定器上以确定的顺序分别固定在其上（探针颗粒）而获得。 多个分别装有探针颗粒的毛细管或凹槽平行地排列，并且将每个毛细管或凹槽中包含的一个颗粒注入另一个毛细管或凹槽中以产生探针阵列，其中各种探针 颗粒以恒定且明确的顺序排列。 通过将各种探针分别附着到不同尺寸的颗粒上同时测量各种荧光团标记的DNA。 由各种固定的DNA探针组成的探针阵列可以容易地生成，并且可以提供用于检测由各种固定的任意DNA探针组成的各种DNA的探针阵列。

    DNA sequencing method and DNA sample preparation method
    8.
    发明授权
    DNA sequencing method and DNA sample preparation method 失效
    DNA测序方法和DNA样品制备方法

    公开(公告)号：US5985556A

    公开(公告)日：1999-11-16

    申请号：US715461

    申请日：1996-09-18

    申请人： Hideki Kambara ,  Kazunori Okano

    发明人： Hideki Kambara ,  Kazunori Okano

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6869 ,  C12N15/1096

    摘要： A kit and a method of DNA sequencing including digesting a sample DNA with a restriction enzyme to obtain a DNA fragment; introducing an oligonucleotide having a definite base sequence into the DNA fragment at the 3' terminus; and performing a complementary strand extension reaction, using a labeled primer. The complementary strand extension reaction uses as a template, a single strand of the DNA fragment having the oligonucleotide introduced thereinto, to obtain a labeled extended primer having a base sequence complementary to the single strand of the DNA fragment. Next a sequencing reaction with the labeled primer and using the single strand of the DNA fragment having the oligonucleotide introduced thereinto, is performed with the extended labeled primer, using as a template, a part of the single strand of the sample DNA having the base sequence of the single strand of the DNA fragment and a contiguous sequence adjacent thereto, or a single strand of the sample DNA. Finally the products of the sequencing reaction are electrophoresed to determine the base sequence of the DNA fragment and at least a part of the base sequence of the sample DNA adjacent to the base sequence of the DNA fragment.

    摘要翻译： 一种试剂盒和DNA测序方法，包括用限制酶消化样品DNA以获得DNA片段; 在3'末端向DNA片段引入具有确定碱基序列的寡核苷酸; 并使用标记的引物进行互补链延伸反应。 互补链延伸反应用作模板，其中引入有寡核苷酸的DNA片段的单链，以获得具有与DNA片段的单链互补的碱基序列的标记的延伸引物。 接下来，使用标记的引物进行测序反应，并使用引入了寡核苷酸的DNA片段的单链进行扩增标记的引物，使用模板，将具有碱基序列的样品DNA的单链的一部分 的DNA片段的单链和与其相邻的连续序列，或样品DNA的单链。 最后将测序反应的产物进行电泳以确定DNA片段的碱基序列和与DNA片段的碱基序列相邻的样品DNA的至少一部分碱基序列。

    Device for separating polynucleotides having a plurality of electrode-containing cells and movable collecting capillary
    9.
    发明授权
    Device for separating polynucleotides having a plurality of electrode-containing cells and movable collecting capillary 失效
    用于分离具有多个含电极的电池和可动集电毛细管的多核苷酸的装置

    公开(公告)号：US5607646A

    公开(公告)日：1997-03-04

    申请号：US410544

    申请日：1995-03-21

    申请人： Kazunori Okano ,  Hideki Kambara

    发明人： Kazunori Okano ,  Hideki Kambara

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  B01L11/00

    CPC分类号： C12Q1/6837 ,  C12Q1/6825 ,  B01J2219/00653 ,  Y10S436/807 ,  Y10S436/809 ,  Y10T436/143333

    摘要： A method for simultaneously detecting a plurality of target polynucleotides in a sample on a single reaction chip and a method for separating a plurality of target polynucleotides are provided. On the reaction chip are arranged a plurality of independent cells for capturing different target polynucleotides. Different probes are immobilized onto the individual cells, and detect the cells where the target polynucleotides are captured, thereby analyzing the species of the captured target polynucleotides. Also, the individual cells function as electrodes to elute the captured target polynucleotides therethrough, thereby separating a plurality of the target polynucleotides.

    摘要翻译： 提供了在单个反应芯片上同时检测样品中的多个靶多核苷酸的方法和分离多个靶多核苷酸的方法。 在反应芯片上布置有用于捕获不同靶多核苷酸的多个独立细胞。 将不同的探针固定在单个细胞上，并检测捕获靶多核苷酸的细胞，从而分析捕获的靶多核苷酸的种类。 此外，各个细胞起电极的作用，从而通过其分离多个靶多核苷酸。

    METHOD AND KIT FOR DETECTING BIOLOGICAL SIGNAL OF THREE-DIMENSIONAL CELL CULTURE MATERIAL
    10.
    发明申请
    METHOD AND KIT FOR DETECTING BIOLOGICAL SIGNAL OF THREE-DIMENSIONAL CELL CULTURE MATERIAL 有权
    检测三维细胞培养物质生物学信号的方法和试剂盒

    公开(公告)号：US20110217726A1

    公开(公告)日：2011-09-08

    申请号：US13128360

    申请日：2009-11-10

    申请人： Michiya Matsusaki ,  Mitsuru Akashi ,  Kazunori Okano

    发明人： Michiya Matsusaki ,  Mitsuru Akashi ,  Kazunori Okano

    IPC分类号： C12Q1/02 ,  C12N5/071

    CPC分类号： G01N33/5032 ,  G01N33/5088 ,  G01N33/54313

    摘要： Disclosed is a method for detecting a biological signal of a three-dimensional cell culture construct. The method for detecting a biological signal of a three-dimensional cell culture construct includes: providing a three-dimensional cell culture construct that contains at least two cell layers laminated to each other and a sensor particle capable of detecting a biological signal; and observing the sensor particle optically. Preferably, the three-dimensional cell culture construct contains an extracellular matrix including a combination of a protein or polymer having an RGD sequence and a protein or polymer that interacts with the protein or polymer having the RGD sequence, or a combination of a protein or polymer that is positively charged and a protein or polymer that is negatively charged.

    摘要翻译： 公开了一种检测三维细胞培养构建体的生物信号的方法。 用于检测三维细胞培养构建体的生物信号的方法包括：提供包含彼此层压的至少两个细胞层的三维细胞培养构建体和能够检测生物信号的传感器粒子; 并且光学地观察传感器粒子。 优选地，三维细胞培养物构建体包含细胞外基质，其包括具有RGD序列的蛋白质或聚合物和与具有RGD序列的蛋白质或聚合物相互作用的蛋白质或聚合物或蛋白质或聚合物的组合的组合 带正电荷的蛋白质或聚合物带负电荷。