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    Fusion proteins
    2.
    发明授权
    Fusion proteins 有权
    融合蛋白

    公开(公告)号：US09243301B2

    公开(公告)日：2016-01-26

    申请号：US13419381

    申请日：2012-03-13

    Applicant: Keith Foster ,  John Chaddock ,  Philip Marks ,  Patrick Stancombe ,  Kei Roger Aoki ,  Joseph Francis ,  Lance Steward

    Inventor： Keith Foster ,  John Chaddock ,  Philip Marks ,  Patrick Stancombe ,  Kei Roger Aoki ,  Joseph Francis ,  Lance Steward

    IPC: A61K38/00 ,  C12N9/52 ,  C12N15/62 ,  C07K14/665

    CPC classification number: C12Y304/24069 ,  A61K38/00 ,  A61K38/1709 ,  A61K38/482 ,  A61K38/4886 ,  A61K38/4893 ,  A61K47/6415 ,  A61K47/65 ,  C07K14/665 ,  C07K2319/055 ,  C07K2319/06 ,  C07K2319/50 ,  C12N9/52 ,  C12N15/62

    Abstract: A single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment can cleave a protein of the exocytic fusion apparatus of a nociceptive sensory afferent; a Targeting Moiety that can bind to a Binding Site on the nociceptive sensory afferent, which Binding Site can undergo endocytosis to be incorporated into an endosome within the nociceptive sensory afferent; a protease cleavage site at which site the fusion protein is cleavable by a protease, which is located between the non-cytotoxic protease and the Targeting Moiety; and a translocation domain that can translocate the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the nociceptive sensory afferent; wherein the Targeting Moiety is BAM, β-endorphin, bradykinin, substance P, dynorphin and/or nociceptin. Nucleic acid sequences encoding the fusion proteins, methods of preparing same and uses thereof are also described.

    Abstract translation: 一种单链多肽融合蛋白，其包含：非细胞毒性蛋白酶或其片段，所述蛋白酶或蛋白酶片段可以切割伤害性感觉传入的胞外融合装置的蛋白质; 可以结合伤害性感觉传入物上的结合位点的靶向物质，其结合位点可以进行内吞作用以掺入伤害性感觉传入体内的内体; 蛋白酶切割位点，位于融合蛋白可被位于非细胞毒性蛋白酶和靶向部位之间的蛋白酶切割; 以及易位区域，其可以将位于内体内的蛋白酶或蛋白酶片段穿过内体膜并转移到伤害性感觉传入物的胞质溶胶中; 其中靶向部分是BAM，β-内啡肽，缓激肽，物质P，强啡肽和/或伤害感受肽。 还描述了编码融合蛋白的核酸序列，其制备方法及其用途。

    Lipophilic dye-based fret assays for clostridal toxin activity
    8.
    发明授权
    Lipophilic dye-based fret assays for clostridal toxin activity 失效
    用于梭菌毒素活性的亲脂性染料基分析法

    公开(公告)号：US08124357B2

    公开(公告)日：2012-02-28

    申请号：US11908438

    申请日：2006-04-04

    Applicant: Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

    Inventor： Ester Fernandez-Salas ,  Lance E. Steward ,  Kei Roger Aoki

    IPC: C12N5/07 ,  C12N15/74 ,  C12Q1/37 ,  C07H21/04

    CPC classification number: G01N33/5014 ,  G01N33/5058 ,  G01N33/542 ,  G01N33/56911 ,  G01N33/94

    Abstract: Compositions useful for detecting Clostridial toxin activity comprising a cell that comprises a membrane-associated Clostridial toxin substrate comprising a first member of a fluorescence resonance energy transfer pair; and a Clostridial toxin recognition sequence including a cleavage site; and a membrane-associated second member of the FRET pair and methods useful for determining Clostridial toxin activity using such Clostridial toxin substrates.

    Abstract translation: 用于检测梭菌毒素活性的组合物，其包含细胞，所述细胞包含与所述膜相关的梭菌毒素底物，所述底物包含荧光共振能量转移对的第一成员; 和包含切割位点的梭菌毒素识别序列; 和FRET对的膜相关的第二个成员以及可用于使用这种梭菌毒素底物测定梭菌毒素活性的方法。

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