公开(公告)号：US07887684B2

公开(公告)日：2011-02-15

申请号：US11174583

申请日：2005-07-06

申请人： Shinya Awano ,  Keisuke Makino ,  Yoshiaki Kuroki ,  Takao Kojima ,  Masashi Ando

发明人： Shinya Awano ,  Keisuke Makino ,  Yoshiaki Kuroki ,  Takao Kojima ,  Masashi Ando

IPC分类号： G01N27/407

CPC分类号： G01N27/4071

摘要： A gas sensor element having an element body, the element body including: a ceramic heater having ceramic layers and a heater element embedded in the ceramic layers; and a solid electrolyte layer including a detection section covered by a pair of electrodes, the solid electrolyte layer being laminated together with the ceramic heater. Furthermore, the element body has a width at a front portion including the detection section smaller than at a rear portion, and at least both side edge faces of the front portion of the element body are covered with a porous layer.

摘要翻译： 一种具有元件体的气体传感器元件，所述元件主体包括：具有陶瓷层的陶瓷加热器和嵌入在所述陶瓷层中的加热元件; 以及包含由一对电极覆盖的检测部的固体电解质层，所述固体电解质层与所述陶瓷加热器层压在一起。 此外，元件主体在包括检测部的前部具有比后部更小的宽度，并且元件主体的前部的至少两个侧边面被多孔层覆盖。

公开(公告)号：US07329844B2

公开(公告)日：2008-02-12

申请号：US10374056

申请日：2003-02-27

申请人： Takao Kojima ,  Keisuke Makino ,  Shinya Awano

发明人： Takao Kojima ,  Keisuke Makino ,  Shinya Awano

IPC分类号： H05B3/44

CPC分类号： G01N27/4067

摘要： A prismatic ceramic heater for heating a gas sensor element includes a heating resistor embedded in ceramic, and has a substantially rectangular cross section. At least part of a longitudinally extending edge portion of the prismatic ceramic heater is located in the vicinity of the heating resistor. This part of the longitudinally extending edge portion is coated with a porous protective layer. The protective layer has a thickness not less than 20 μm and a curved surface of a curvature radius not less than 10 μm. The protective layer prevents cracking induced by contact with water.

摘要翻译： 用于加热气体传感器元件的棱柱陶瓷加热器包括嵌入陶瓷的加热电阻器，并且具有基本上矩形的横截面。 棱柱形陶瓷加热器的纵向延伸边缘部分的至少一部分位于加热电阻器附近。 纵向延伸的边缘部分的该部分涂覆有多孔保护层。 保护层的厚度不小于20μm，曲率半径不小于10μm的曲面。 保护层防止与水接触引起的裂纹。

公开(公告)号：US20110027847A1

公开(公告)日：2011-02-03

申请号：US12864360

申请日：2009-01-22

申请人： Akinori Matsushika ,  Shigeki Sawayama ,  Hiroyuki Inoue ,  Keisuke Makino ,  Tsutomu Kodaki ,  Seiya Watanabe

发明人： Akinori Matsushika ,  Shigeki Sawayama ,  Hiroyuki Inoue ,  Keisuke Makino ,  Tsutomu Kodaki ,  Seiya Watanabe

IPC分类号： C12P7/06 ,  C12N1/19

CPC分类号： C12N15/81 ,  C12P7/06 ,  Y02E50/17

摘要： Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of producing ethanol from xylose in high yields, and having high xylose fermentability in the presence of glucose, as well as a method using the recombinant yeast for highly efficiently producing ethanol from xylose or a saccharified solution from lignocellulose-based biomass are provided. Furthermore, a method for improving the xylose fermentability of the genetic recombinant yeast of the present invention via acclimatization treatment is also provided herein.

摘要翻译： 提供表达木糖还原酶（XR），（野生型或突变体）木糖醇脱氢酶（XDH）和木酮糖激酶（XK）的遗传重组酵母和使用酵母从木糖高效生产乙醇的方法。 通过染色体整合引入派生芽孢杆菌衍生的XR和（野生型或修饰型）XDH基因和酿酒酵母衍生的XK基因。 因此，具有高木糖发酵速率的基因重组酵母，能够以高产率从木糖产生乙醇，并且在葡萄糖存在下具有高木糖发酵能力，以及使用重组酵母高效生产乙醇的方法 提供木糖或基于木质纤维素的生物质的糖化溶液。 此外，还提供了通过驯化处理改善本发明的遗传重组酵母的木糖发酵能力的方法。

公开(公告)号：US5939256A

公开(公告)日：1999-08-17

申请号：US683918

申请日：1996-07-19

申请人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Masahiro Kawaguchi ,  Keisuke Makino ,  Akira Murakami

发明人： Nobuko Yamamoto ,  Tadashi Okamoto ,  Yoshinori Tomida ,  Masahiro Kawaguchi ,  Keisuke Makino ,  Akira Murakami

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6818

摘要： A method for detecting a nucleic acid hybrid comprises steps of adding a nucleic acid probe into a sample solution containing a targeted nucleic acid, and detecting a double helical structure of a hybrid formed between the probe and the targeted nucleic acid, wherein the step for detecting the double helical structure comprises incorporating, into the sample solution, two or more kinds of reagents which are capable of causing a detectable change by interaction therebetween through the double helical structure and measuring the change caused by the interaction of the reagents; and at least one of the two or more kinds of reagents is joined to the probe.

摘要翻译： 检测核酸杂交物的方法包括以下步骤：将核酸探针加入到含有靶核酸的样品溶液中，并检测在探针与靶向核酸之间形成的杂交体的双螺旋结构，其中检测步骤 双螺旋结构包括在样品溶液中加入两种或更多种试剂，其能够通过双螺旋结构通过相互作用引起可检测的变化并测量由试剂的相互作用引起的变化; 并且两种或更多种试剂中的至少一种被连接到探针。

公开(公告)号：US4173821A

公开(公告)日：1979-11-13

申请号：US827091

申请日：1977-08-23

申请人： Masatoshi Yamamoto ,  Keisuke Makino ,  Kiyomi Suzukawa ,  Souichirou Nakamura ,  Kiyomi Sakai

发明人： Masatoshi Yamamoto ,  Keisuke Makino ,  Kiyomi Suzukawa ,  Souichirou Nakamura ,  Kiyomi Sakai

IPC分类号： H01L23/48 ,  H01L21/50 ,  H01L21/56 ,  H01L21/60 ,  B01J17/00

CPC分类号： H01L24/86 ,  H01L21/56 ,  H01L24/29 ,  H01L24/75 ,  H01L24/83 ,  H01L2224/2919 ,  H01L2224/8319 ,  H01L2224/83801 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01033 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/014 ,  H01L2924/181 ,  Y10T29/49004 ,  Y10T29/49121

摘要： In the process of fabrication of semiconductor devices, a lead frame hoop supplied from a supply reel is set forward to successively enter into a series of fabricating steps. After having been subjected to pellet bonding, wire bonding and resin-molding, it is wound up about a take-up reel. Baking step is performed with the lead frame hoop wound on the take-up reel. The lead frame hoop unwound from the take-up reel is cut into separate individual semiconductor device units from one another and the measurement of characteristics and the classification of the thus prepared units are carried out with the positions of the units maintained as they were at the cutting step. Accordingly, the process has a smaller number of steps and therefore the production cost is reduced, and the speed of working is improved.

摘要翻译： 在制造半导体器件的过程中，从供带盘供给的引线框环向前设置以依次进入一系列制造步骤。 在进行颗粒粘合，引线接合和树脂成型之后，将其卷绕在卷绕卷轴上。 烘烤步骤是用引导框架缠绕在卷取卷轴上进行的。 将从卷取卷轴展开的引导框架环彼此切割为独立的单独的半导体器件单元，并且由此将所准备的单元的特性和分类的测量结果按照它们在 切割步骤 因此，该方法具有较少的步骤数，因此降低了生产成本，并提高了加工速度。

公开(公告)号：US6162806A

公开(公告)日：2000-12-19

申请号：US51054

申请日：1998-03-31

申请人： Toshiyuki Arai ,  Keisuke Makino ,  Kenjiro Mori

发明人： Toshiyuki Arai ,  Keisuke Makino ,  Kenjiro Mori

IPC分类号： A61K8/49 ,  A61K31/519 ,  A61Q17/04 ,  C07D475/04 ,  A61K31/495 ,  A61K31/505 ,  C07D475/00

CPC分类号： A61Q17/04 ,  A61K8/4953 ,  A61K31/519 ,  A61K2800/522 ,  C07D475/04

摘要： An active oxygen scavenger comprising as an active ingredient a pterin derivative of the formula (I): ##STR1## wherein R.sup.1 and R.sup.2 are independently a hydrogen atom, an alkyl group of 1 to 4 carbon atoms, or an acyl group of the formula R.sup.3 --CO--, R.sup.3 is an alkyl group of 1 to 4 carbon atoms, X is a formyl group or hydroxymethyl group, A is a group of the formula (Ia): ##STR2## and n is 0 or an integer of 1 or more, with the proviso that when X is a hydroxymethyl group, n is 0, or when n is an integer of 1 or more, each of R.sup.1 and R.sup.2 is a hydrogen atoms, and X is a formyl group, or a cyclic compound thereof, or a salt thereof is disclosed.

摘要翻译： PCT No.PCT / JP97 / 02649 Sec。 371日期1998年3月31日 102（e）1998年3月31日PCT PCT 1997年7月30日PCT公布。 第WO98 / 04558号公报 日期：1998年5月2日，含活性成分的活性氧清除剂为式（I）的蝶呤衍生物：其中R1和R2独立地为氢原子，1-4个碳原子的烷基或式 R3-CO-，R3是1〜4个碳原子的烷基，X是甲酰基或羟甲基，A是式（Ia）的基团，n是0或1以上的整数，与 条件是当X为羟甲基时，n为0，n为1以上的整数时，R 1，R 2为氢原子，X为甲酰基或其环状化合物，或 公开了其盐。

公开(公告)号：US5705346A

公开(公告)日：1998-01-06

申请号：US671829

申请日：1996-06-25

申请人： Tadashi Okamoto ,  Yoshinori Tomida ,  Nobuko Yamamoto ,  Masahiro Kawaguchi ,  Keisuke Makino ,  Akira Murakami

发明人： Tadashi Okamoto ,  Yoshinori Tomida ,  Nobuko Yamamoto ,  Masahiro Kawaguchi ,  Keisuke Makino ,  Akira Murakami

IPC分类号： A61B10/00 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01N24/00 ,  G01N33/58

CPC分类号： C12Q1/6818 ,  Y10S435/81

摘要： A method for detecting a target nucleic acid comprises the steps of reacting a sample with a probe in the presence of two or more kinds of reagents capable of being made an irreversible change capable of being detected and accumulating by an interaction through a double helix structure under a condition enabling the replication of the formation and dissociation of a hybrid composed of the target nucleic acid in the sample and the probe, accumulating the irreversible change caused by the interaction of the reagents, and then detecting the accumulated change.

摘要翻译： 用于检测靶核酸的方法包括以下步骤：在两种或多种能够被制成不可逆变化的试剂的存在下使样品与探针反应，所述试剂能够通过双螺旋结构的相互作用被检测和累积 使得能够复制由样品和探针中的靶核酸构成的杂合物的形成和解离，累积由试剂的相互作用引起的不可逆变化，然后检测累积的变化。

公开(公告)号：US08445243B2

公开(公告)日：2013-05-21

申请号：US12864360

申请日：2009-01-22

申请人： Akinori Matsushika ,  Shigeki Sawayama ,  Hiroyuki Inoue ,  Keisuke Makino ,  Tsutomu Kodaki ,  Seiya Watanabe

发明人： Akinori Matsushika ,  Shigeki Sawayama ,  Hiroyuki Inoue ,  Keisuke Makino ,  Tsutomu Kodaki ,  Seiya Watanabe

IPC分类号： C12P7/06 ,  C12N1/14

CPC分类号： C12N15/81 ,  C12P7/06 ,  Y02E50/17

摘要： Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of producing ethanol from xylose in high yields, and having high xylose fermentability in the presence of glucose, as well as a method using the recombinant yeast for highly efficiently producing ethanol from xylose or a saccharified solution from lignocellulose-based biomass are provided. Furthermore, a method for improving the xylose fermentability of the genetic recombinant yeast of the present invention via acclimatization treatment is also provided herein.

摘要翻译： 提供表达木糖还原酶（XR），（野生型或突变体）木糖醇脱氢酶（XDH）和木酮糖激酶（XK）的遗传重组酵母和使用酵母从木糖高效生产乙醇的方法。 通过染色体整合引入派生芽孢杆菌衍生的XR和（野生型或修饰型）XDH基因和酿酒酵母衍生的XK基因。 因此，具有高木糖发酵速率的基因重组酵母，能够以高产率从木糖产生乙醇，并且在葡萄糖存在下具有高木糖发酵能力，以及使用重组酵母高效生产乙醇的方法 提供木糖或基于木质纤维素的生物质的糖化溶液。 此外，还提供了通过驯化处理改善本发明的遗传重组酵母的木糖发酵能力的方法。

公开(公告)号：US20080070795A1

公开(公告)日：2008-03-20

申请号：US11851865

申请日：2007-09-07

申请人： Keisuke Makino ,  Tsutomu Kodaki ,  Seiya Watanabe ,  Seung Pack ,  Mitsuru Nonogawa ,  Hiroo Wada ,  Yasuko Yoshida ,  Kazunari Yamada

发明人： Keisuke Makino ,  Tsutomu Kodaki ,  Seiya Watanabe ,  Seung Pack ,  Mitsuru Nonogawa ,  Hiroo Wada ,  Yasuko Yoshida ,  Kazunari Yamada

IPC分类号： C40B30/06 ,  C07H21/04 ,  C40B40/08

CPC分类号： C07D498/04 ,  C07H19/24 ,  C07H21/04

摘要： The present invention aims to provide a technique for stably immobilizing a probe on a carrier The present invention provides a linker compound represented by formula (1): wherein A represents a hydrogen atom, another substituent, a sugar, or a derivative thereof.

摘要翻译： 本发明的目的在于提供一种用于将探针稳定地固定在载体上的技术。本发明提供由式（1）表示的连接剂化合物：其中A表示氢原子，另一取代基，糖或其衍生物。

公开(公告)号：US20070007136A1

公开(公告)日：2007-01-11

申请号：US11174583

申请日：2005-07-06

申请人： Shinya Awano ,  Keisuke Makino ,  Yoshiaki Kuroki ,  Takao Kojima ,  Masashi Ando

发明人： Shinya Awano ,  Keisuke Makino ,  Yoshiaki Kuroki ,  Takao Kojima ,  Masashi Ando

IPC分类号： G01N27/26

CPC分类号： G01N27/4071

摘要： A gas sensor element having an element body, the element body including: a ceramic heater having ceramic layers and a heater element embedded in the ceramic layers; and a solid electrolyte layer including a detection section covered by a pair of electrodes, the solid electrolyte layer being laminated together with the ceramic heater. Furthermore, the element body has a width at a front portion including the detection section smaller than at a rear portion, and at least both side edge faces of the front portion of the element body are covered with a porous layer.

摘要翻译： 一种具有元件体的气体传感器元件，所述元件主体包括：具有陶瓷层的陶瓷加热器和嵌入在所述陶瓷层中的加热元件; 以及包含由一对电极覆盖的检测部的固体电解质层，所述固体电解质层与所述陶瓷加热器层压在一起。 此外，元件主体在包括检测部的前部具有比后部更小的宽度，并且元件主体的前部的至少两个侧边面被多孔层覆盖。