-
公开(公告)号：US20090077678A1
公开(公告)日：2009-03-19
申请号：US12272471
申请日：2008-11-17
Applicant: Keizaburo Miki , Johji Miwa , Nozomu Isowa
Inventor： Keizaburo Miki , Johji Miwa , Nozomu Isowa
IPC: A01K67/00
CPC classification number: C07K14/43595 , A01K67/0334 , A01K2217/05
Abstract: A transgenic mollusk which can express a desired foreign gene, and a method for producing the same are disclosed. The mollusk is a transgenic mollusk into which a desired foreign gene (excluding a gene giving resistance to a virus) is introduced, which expresses the foreign gene. This transgenic mollusk can be produced by microinjecting into gonad of male and/or female of mollusk a recombinant vector into which a desired foreign gene to be introduced or a nucleic acid containing the foreign gene is inserted; crossing the male and female to produce individuals of first generation; and selecting therefrom (an) individual(s) which express(es) the desired gene.
Abstract translation: 公开了可以表达所需外源基因的转基因软体动物及其制备方法。 软体动物是一种转基因软体动物，其中引入了期望的外源基因（不包括给病毒抗性的基因），其表达外源基因。 该转基因软体动物可以通过向软体动物的雄性和/或雌性的性腺中进行显微注射，其中插入有待引入的期望的外源基因或含有外来基因的核酸的重组载体; 穿越男女生产第一代人; 并从中选择（a）表达所需基因的个体。
-
2.发明申请THREE-DIMENSIONAL CELL CULTURE CARRIER AND METHOD FOR CELL CULTURE USING THE SAME 审中-公开三维细胞培养载体和使用该细胞培养物的细胞培养方法
公开(公告)号：US20100041146A1
公开(公告)日：2010-02-18
申请号：US12523003
申请日：2008-01-11
Applicant: Hiroshi Kambayashi , Masanobu Tsuda , Noriaki Sato , Keizaburo Miki , Nanako Mitani
Inventor： Hiroshi Kambayashi , Masanobu Tsuda , Noriaki Sato , Keizaburo Miki , Nanako Mitani
CPC classification number: C12N5/0062 , C12N5/0068 , C12N2533/12
Abstract: A three-dimensional cell culture carrier of the present invention includes a fibrous structure having a three-dimensional space for cell culture. The fibrous structure is formed of a plurality of intertangled fibers. Each of the fibers used in the fibrous structure is made of a material having a visible light transmittance of 40% or more (preferably 50% or more) when it is formed into a shape having a thickness of 3 mm. Examples of such fibers include glass fibers. The fiber has an aspect ratio of 1 or more, and a fiber diameter of 100 μm to 700 μm.
Abstract translation: 本发明的三维细胞培养载体包括具有用于细胞培养的三维空间的纤维结构体。 纤维结构由多个交错的纤维形成。 纤维结构体中使用的纤维，当形成厚度为3mm的形状时，其可见光透射率为40％以上（优选为50％以上）的材料构成。 这种纤维的实例包括玻璃纤维。 纤维的纵横比为1以上，纤维直径为100μm〜700μm。
-
公开(公告)号：US20050198697A1
公开(公告)日：2005-09-08
申请号：US11094447
申请日：2005-03-31
Applicant: Keizaburo Miki , Johji Miwa , Nozomu Isowa
Inventor： Keizaburo Miki , Johji Miwa , Nozomu Isowa
IPC: C12N15/09 , A01K67/033 , C07K14/435 , C12N5/10
CPC classification number: C07K14/43595 , A01K67/0334 , A01K2217/05
Abstract: A transgenic mollusk which can express a desired foreign gene, and a method for producing the same are disclosed. The mollusk is a transgenic mollusk into which a desired foreign gene (excluding a gene giving resistance to a virus) is introduced, which expresses the foreign gene. This transgenic mollusk can be produced by microinjecting into gonad of male and/or female of mollusk a recombinant vector into which a desired foreign gene to be introduced or a nucleic acid containing the foreign gene is inserted; crossing the male and female to produce individuals of first generation; and selecting therefrom (an) individual(s) which express(es) the desired gene.
Abstract translation: 公开了可以表达所需外源基因的转基因软体动物及其制备方法。 软体动物是一种转基因软体动物，其中引入了期望的外源基因（不包括给病毒抗性的基因），其表达外源基因。 该转基因软体动物可以通过向软体动物的雄性和/或雌性的性腺中进行显微注射，其中插入有待引入的期望的外源基因或含有外来基因的核酸的重组载体; 穿越男女生产第一代人; 并从中选择（a）表达所需基因的个体。
-
公开(公告)号：US20100218263A1
公开(公告)日：2010-08-26
申请号：US12775904
申请日：2010-05-07
Applicant: Keizaburo Miki , Johji Miwa , Nozomu Isowa
Inventor： Keizaburo Miki , Johji Miwa , Nozomu Isowa
IPC: A01K67/033
CPC classification number: C07K14/43595 , A01K67/0334 , A01K2217/05
Abstract: A transgenic mollusk which can express a desired foreign gene, and a method for producing the same are disclosed. The mollusk is a transgenic mollusk into which a desired foreign gene (excluding a gene giving resistance to a virus) is introduced, which expresses the foreign gene. This transgenic mollusk can be produced by microinjecting into gonad of male and/or female of mollusk a recombinant vector into which a desired foreign gene to be introduced or a nucleic acid containing the foreign gene is inserted; crossing the male and female to produce individuals of first generation; and selecting therefrom (an) individual(s) which express(es) the desired gene.
Abstract translation: 公开了可以表达所需外源基因的转基因软体动物及其制备方法。 软体动物是一种转基因软体动物，其中引入了期望的外源基因（不包括给病毒抗性的基因），其表达外源基因。 该转基因软体动物可以通过向软体动物的雄性和/或雌性的性腺中进行显微注射，其中插入有待引入的期望的外源基因或含有外来基因的核酸的重组载体; 穿越男女生产第一代人; 并从中选择（a）表达所需基因的个体。
-
公开(公告)号：US20090307789A1
公开(公告)日：2009-12-10
申请号：US12544603
申请日：2009-08-20
Applicant: Keizaburo MIKI , Johji Miwa , Nozomu Isowa
Inventor： Keizaburo MIKI , Johji Miwa , Nozomu Isowa
IPC: A01K67/033 , C12N15/85
CPC classification number: C07K14/43595 , A01K67/0334 , A01K2217/05
Abstract: A transgenic mollusk which can express a desired foreign gene, and a method for producing the same are disclosed. The mollusk is a transgenic mollusk into which a desired foreign gene (excluding a gene giving resistance to a virus) is introduced, which expresses the foreign gene. This transgenic mollusk can be produced by microinjecting into gonad of male and/or female of mollusk a recombinant vector into which a desired foreign gene to be introduced or a nucleic acid containing the foreign gene is inserted; crossing the male and female to produce individuals of first generation; and selecting therefrom (an) individual(s) which express(es) the desired gene.
-
公开(公告)号：US20090271882A1
公开(公告)日：2009-10-29
申请号：US11992317
申请日：2006-08-24
Applicant: Kazuhiro Umeyama , Hiroshi Nagashima , Masahito Watanabe , Keizaburo Miki
Inventor： Kazuhiro Umeyama , Hiroshi Nagashima , Masahito Watanabe , Keizaburo Miki
IPC: A01K67/027 , A01K67/02
CPC classification number: A01K67/0275 , A01K2217/05 , A01K2227/108 , A01K2267/0325 , A01K2267/0362 , C07K14/4702 , C12N15/8509 , C12N2830/008 , C12N2830/40
Abstract: A transgenic animal having diabetes, which is more suitable as a model of human than rodents, and its preparation method are disclosed. The method for preparing the transgenic pig comprises introducing a nucleic acid into a fertilized egg, clonal egg or embryo, the nucleic acid comprising a foreign gene which contains a region encoding dimerization domain of hepatocyte nuclear factor-1α, but does not encode a normal hepatocyte nuclear factor-1α, and a promoter located upstream of the foreign gene, which promoter is capable of expressing the foreign gene in a pig cell; and developing an individual from the fertilized egg, clonal egg or embryo.
Abstract translation: 公开了一种具有比啮齿动物更适合作为人类模型的糖尿病的转基因动物及其制备方法。 制备转基因猪的方法包括将核酸引入受精卵，克隆卵或胚胎中，所述核酸包含含有编码肝细胞核因子-1alpha的二聚化结构域但不编码正常肝细胞的区域的外源基因 核因子-1alpha和位于外源基因上游的启动子，该启动子能够在猪细胞中表达外源基因; 并从受精卵，克隆蛋或胚胎培养个体。
-
公开(公告)号：US5552274A
公开(公告)日：1996-09-03
申请号：US117329
申请日：1993-09-07
Applicant: Noboru Oyama , Shuichiro Yamaguchi , Takeshi Shimomura , Keizaburo Miki
Inventor： Noboru Oyama , Shuichiro Yamaguchi , Takeshi Shimomura , Keizaburo Miki
CPC classification number: G01N29/12 , C12Q1/6825 , G01N2291/02466 , Y10T436/143333
Abstract: Methods for detecting target DNA's in a sample on the basis of oscillation frequency using nucleic acid sequences immobilized to the surface of an electrode of an elastic wave element, and DNA sensors for conducting the methods are described. These methods provide for highly sensitive detection of target DNA's in a sample.
Abstract translation: 基于振荡频率检测样品中靶DNA的方法，使用固定在弹性波元件的电极表面的核酸序列，以及用于进行该方法的DNA传感器。 这些方法提供样品中目标DNA的高度灵敏的检测。
-
公开(公告)号：US4992374A
公开(公告)日：1991-02-12
申请号：US79680
申请日：1987-07-30
Applicant: Masanobu Sugimoto , Fukumi Nishimaki , Tadashi Maruyama , Keizaburo Miki , Michio Morita , Kazuyoshi Suzuki
Inventor： Masanobu Sugimoto , Fukumi Nishimaki , Tadashi Maruyama , Keizaburo Miki , Michio Morita , Kazuyoshi Suzuki
IPC: C12N15/09 , A61K39/00 , A61K39/285 , C12N7/00 , C12N7/06 , C12N15/00 , C12N15/863
CPC classification number: C12N15/86 , C12N15/00 , C12N7/00 , C12N2710/24121 , C12N2710/24143 , C12N2710/24162
Abstract: An improved mutant vaccinia virus providing a pock and plaque size on RK13 cells that is approximately the same as those of the Lister original, having a proliferation potency on YTV cells that is approximately the same as that of the Lister original, and having a neurovirulence, assessed by a recovery of an intrabrain virus, that is lower than that of the Lister original; and a process for the production thereof.
