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1.发明授权公开(公告)号：US07316903B2
公开(公告)日：2008-01-08
申请号：US10809688
申请日：2004-03-26
CPC分类号： C12Q1/6827 , C12Q2521/507
摘要： The present invention relates, e.g., to a method of detecting a mismatch in a double stranded nucleic acid target, comprising (a) contacting the target with (i) a Mu-end nucleic acid, and (ii) a phage Mu transposase, under conditions effective for the Mu-end nucleic acid to transpose into the target at about the site of a mismatch, if the target comprises a mismatch, and (b) detecting transposition of the Mu-end DNA into the target, wherein transposition of the Mu-end nucleic acid into the target at a predominant site indicates the presence of a mismatch at that site.
摘要翻译： 本发明涉及例如检测双链核酸靶的错配的方法，其包括（a）使靶与（i）Mu末端核酸和（ii）噬菌体Mu转座酶接触， 如果目标包含不匹配，则（b）检测Mu末端DNA转移到靶中，使Mu端核酸在大约位点处转移到靶中有效的条件，其中转移Mu 在主要位置将一个核酸引入靶标，表明在该位点存在失配。
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公开(公告)号：US4716105A
公开(公告)日：1987-12-29
申请号：US680992
申请日：1984-12-13
CPC分类号： C12Q1/6869 , C12N15/00
摘要： The present invention discloses a rapid method of sequencing a relatively large segment of deoxyribonucleic acid. The method in part comprises high frequency insertion of a suitable transposon into a segment of DNA of interest. Preferable use of Mu transposons is described. A plasmid having mini-Mu transposons has been prepared and disclosed.
摘要翻译： 本发明公开了对相对大的脱氧核糖核酸酶进行测序的快速方法。 该方法部分地包括将合适的转座子高频插入感兴趣的DNA片段中。 描述了优选使用Mu转座子。 已经制备并公开了具有迷你-Mo转座子的质粒。
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